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Too much DNA or impurities can stress cells. An optimal DNA amount is better.
Step 2
Why this answer is correct
The correct answer is A. Cells stress हो सकती हैं / Cells can be stressed. Too much DNA or impurities can stress cells. An optimal DNA amount is better.
Step 3
Exam Tip
बहुत अधिक DNA या impurities cells पर stress डाल सकते हैं। Optimal DNA amount better होता है।
C. Cells stress हो सकती हैं और efficiency घट सकती है/Cells may get stressed and efficiency may decrease
Step 1
Concept
Excess DNA or impurities can stress cells. An optimal amount is better for transformation efficiency.
Step 2
Why this answer is correct
The correct answer is C. Cells stress हो सकती हैं और efficiency घट सकती है / Cells may get stressed and efficiency may decrease. Excess DNA or impurities can stress cells. An optimal amount is better for transformation efficiency.
Step 3
Exam Tip
Excess DNA या impurities cells को stress कर सकती हैं। Optimal amount transformation efficiency के लिए बेहतर होता है।
A. खंडों को देखना कठिन हो सकता है/It may be difficult to see fragments
Step 1
Concept
With little DNA the bands may look weak. Adequate sample is important in experiments.
Step 2
Why this answer is correct
The correct answer is A. खंडों को देखना कठिन हो सकता है / It may be difficult to see fragments. With little DNA the bands may look weak. Adequate sample is important in experiments.
Step 3
Exam Tip
कम डीएनए से बैंड कमजोर दिख सकते हैं। प्रयोग में पर्याप्त नमूना महत्वपूर्ण होता है।
B. accurate DNA quantification confuse हो सकती है/Accurate DNA quantification can be confused
Step 1
Concept
RNA contamination can affect DNA amount estimation. RNase treatment or cleanup can help.
Step 2
Why this answer is correct
The correct answer is B. accurate DNA quantification confuse हो सकती है / Accurate DNA quantification can be confused. RNA contamination can affect DNA amount estimation. RNase treatment or cleanup can help.
Step 3
Exam Tip
RNA contamination DNA amount estimate को प्रभावित कर सकती है। RNase treatment या cleanup helpful हो सकता है।
B. enzyme activity amount estimate करने में/In estimating enzyme activity amount
Step 1
Concept
Units describe enzyme activity in a standard way. Units are chosen according to DNA amount.
Step 2
Why this answer is correct
The correct answer is B. enzyme activity amount estimate करने में / In estimating enzyme activity amount. Units describe enzyme activity in a standard way. Units are chosen according to DNA amount.
Step 3
Exam Tip
Units enzyme activity को standard way में बताते हैं। DNA amount के अनुसार units चुने जाते हैं।
A. DNA amount in band का अनुमान/Estimate of DNA amount in band
Step 1
Concept
Band brightness is related to DNA mass. It gives a rough estimate but not exact quantification.
Step 2
Why this answer is correct
The correct answer is A. DNA amount in band का अनुमान / Estimate of DNA amount in band. Band brightness is related to DNA mass. It gives a rough estimate but not exact quantification.
Step 3
Exam Tip
Band brightness DNA mass से जुड़ी होती है। यह exact quantification नहीं पर rough estimate देती है।
A. different reactions में equal DNA amount रखने के लिए/To keep equal DNA amount in different reactions
Step 1
Concept
Equal DNA amount makes comparison fair. This improves interpretation of gel band intensity.
Step 2
Why this answer is correct
The correct answer is A. different reactions में equal DNA amount रखने के लिए / To keep equal DNA amount in different reactions. Equal DNA amount makes comparison fair. This improves interpretation of gel band intensity.
Step 3
Exam Tip
Equal DNA amount comparison को fair बनाता है। इससे gel band intensity interpretation बेहतर होती है।
A. enzyme और DNA amount सही रखने के लिए/To keep enzyme and DNA amount correct
Step 1
Concept
Knowing DNA amount helps set enzyme units and reaction correctly. This makes digestion reliable.
Step 2
Why this answer is correct
The correct answer is A. enzyme और DNA amount सही रखने के लिए / To keep enzyme and DNA amount correct. Knowing DNA amount helps set enzyme units and reaction correctly. This makes digestion reliable.
Step 3
Exam Tip
DNA amount जानकर enzyme units और reaction setup सही किया जाता है। इससे digestion reliable होती है।
A. bands smear या distorted हो सकते हैं/Bands may smear or distort
Step 1
Concept
Overloading the gel can make band interpretation difficult. Load proper DNA amount.
Step 2
Why this answer is correct
The correct answer is A. bands smear या distorted हो सकते हैं / Bands may smear or distort. Overloading the gel can make band interpretation difficult. Load proper DNA amount.
Step 3
Exam Tip
Overloading gel band interpretation को कठिन बना सकता है। Proper DNA amount load करें।
A. enzyme insufficient होकर incomplete digestion दे सकता है/Enzyme may become insufficient and give incomplete digestion
Step 1
Concept
DNA amount should match enzyme capacity. Too much DNA can make complete digestion difficult.
Step 2
Why this answer is correct
The correct answer is A. enzyme insufficient होकर incomplete digestion दे सकता है / Enzyme may become insufficient and give incomplete digestion. DNA amount should match enzyme capacity. Too much DNA can make complete digestion difficult.
Step 3
Exam Tip
DNA amount enzyme capacity से match होना चाहिए। बहुत अधिक DNA complete digestion को कठिन कर सकता है।
A. insert absent या digestion failed हो सकता है/Insert may be absent or digestion failed
Step 1
Concept
Absence of expected band can indicate wrong clone partial digestion or low DNA amount. Confirm with controls.
Step 2
Why this answer is correct
The correct answer is A. insert absent या digestion failed हो सकता है / Insert may be absent or digestion failed. Absence of expected band can indicate wrong clone partial digestion or low DNA amount. Confirm with controls.
Step 3
Exam Tip
Expected band न होना wrong clone partial digestion या low DNA amount का संकेत हो सकता है। Controls से confirm करें।
C. defined condition में DNA काटने की activity amount/Amount of activity to cut DNA under defined condition
Step 1
Concept
Enzyme units describe activity. Enzyme amount is chosen according to DNA amount in reaction setup.
Step 2
Why this answer is correct
The correct answer is C. defined condition में DNA काटने की activity amount / Amount of activity to cut DNA under defined condition. Enzyme units describe activity. Enzyme amount is chosen according to DNA amount in reaction setup.
Step 3
Exam Tip
Enzyme units activity बताते हैं। Reaction setup में DNA amount के अनुसार enzyme मात्रा चुनी जाती है।
A. yield purity ratio और storage detail/Yield purity ratio and storage detail
Step 1
Concept
It is useful to record DNA amount purity ratios and storage condition in a report. This helps future use.
Step 2
Why this answer is correct
The correct answer is A. yield purity ratio और storage detail / Yield purity ratio and storage detail. It is useful to record DNA amount purity ratios and storage condition in a report. This helps future use.
Step 3
Exam Tip
Report में DNA amount purity ratios और storage condition लिखना useful है। इससे future use आसान होता है।
A. low-bind tubes और careful pipetting/Low-bind tubes and careful pipetting
Step 1
Concept
In low DNA amount sample loss is a major issue. Low-bind tubes and careful handling preserve recovery.
Step 2
Why this answer is correct
The correct answer is A. low-bind tubes और careful pipetting / Low-bind tubes and careful pipetting. In low DNA amount sample loss is a major issue. Low-bind tubes and careful handling preserve recovery.
Step 3
Exam Tip
Low DNA amount में sample loss बड़ा issue होता है। Low-bind tubes और careful handling recovery बचाते हैं।
A. small DNA amounts में गलत profile आ सकता है/Wrong profile can occur with small DNA amounts
Step 1
Concept
Forensic samples can have low DNA amount. Contamination can make the result wrong.
Step 2
Why this answer is correct
The correct answer is A. small DNA amounts में गलत profile आ सकता है / Wrong profile can occur with small DNA amounts. Forensic samples can have low DNA amount. Contamination can make the result wrong.
Step 3
Exam Tip
Forensic samples में DNA amount कम हो सकता है। Contamination result को गलत कर सकती है।
Low sample amount can give less DNA. Careful recovery is needed for low-yield samples.
Step 2
Why this answer is correct
The correct answer is A. DNA yield कम हो सकती है / DNA yield may be low. Low sample amount can give less DNA. Careful recovery is needed for low-yield samples.
Step 3
Exam Tip
कम sample से DNA amount कम मिल सकता है। Low-yield samples में careful recovery जरूरी है।
A. band intensity से approximate amount/Approximate amount from band intensity
Step 1
Concept
Band intensity can be compared with a known ladder or standard for approximate DNA amount. It is a rough estimate.
Step 2
Why this answer is correct
The correct answer is A. band intensity से approximate amount / Approximate amount from band intensity. Band intensity can be compared with a known ladder or standard for approximate DNA amount. It is a rough estimate.
Step 3
Exam Tip
Known ladder या standard से band intensity compare कर approximate DNA amount देख सकते हैं। यह rough estimate है।
D. specific double-stranded DNA amount जानना हो/When specific double-stranded DNA amount is needed
Step 1
Concept
Fluorometric methods can use DNA-specific dyes. This can be more specific among contaminants.
Step 2
Why this answer is correct
The correct answer is D. specific double-stranded DNA amount जानना हो / When specific double-stranded DNA amount is needed. Fluorometric methods can use DNA-specific dyes. This can be more specific among contaminants.
Step 3
Exam Tip
Fluorometric methods DNA-specific dyes use कर सकते हैं। यह contaminants के बीच अधिक specific हो सकता है।
A. DNA purity और concentration/DNA purity and concentration
Step 1
Concept
Correct DNA amount and purity are needed for PCR template. Contaminants can inhibit amplification.
Step 2
Why this answer is correct
The correct answer is A. DNA purity और concentration / DNA purity and concentration. Correct DNA amount and purity are needed for PCR template. Contaminants can inhibit amplification.
Step 3
Exam Tip
PCR template के लिए सही DNA amount और purity जरूरी हैं। Contaminants amplification रोक सकते हैं।
D. DNA concentration और purity estimate करने के लिए/To estimate DNA concentration and purity
Step 1
Concept
A spectrophotometer estimates DNA amount and purity using absorbance reading. It is useful in quality control.
Step 2
Why this answer is correct
The correct answer is D. DNA concentration और purity estimate करने के लिए / To estimate DNA concentration and purity. A spectrophotometer estimates DNA amount and purity using absorbance reading. It is useful in quality control.
Step 3
Exam Tip
Spectrophotometer absorbance reading से DNA amount और purity का अनुमान देता है। यह quality control में useful है।
A. DNA purity और concentration/DNA purity and concentration
Step 1
Concept
DNA amount and purity are important for PCR template. Contaminants can inhibit PCR.
Step 2
Why this answer is correct
The correct answer is A. DNA purity और concentration / DNA purity and concentration. DNA amount and purity are important for PCR template. Contaminants can inhibit PCR.
Step 3
Exam Tip
PCR template के लिए DNA amount और purity important हैं। Contaminants PCR inhibit कर सकते हैं।
A. यह downstream reactions में बाधा डाल सकती है/It can interfere with downstream reactions
Step 1
Concept
Protein contamination can affect enzyme reactions and purity readings. Protein removal is necessary.
Step 2
Why this answer is correct
The correct answer is A. यह downstream reactions में बाधा डाल सकती है / It can interfere with downstream reactions. Protein contamination can affect enzyme reactions and purity readings. Protein removal is necessary.
Step 3
Exam Tip
Protein contamination enzyme reactions और purity readings को प्रभावित कर सकती है। Protein removal जरूरी है।
A spectrophotometer estimates DNA amount and purity using absorbance. Link it with quality check.
Step 2
Why this answer is correct
The correct answer is B. स्पेक्ट्रोफोटोमीटर / Spectrophotometer. A spectrophotometer estimates DNA amount and purity using absorbance. Link it with quality check.
Step 3
Exam Tip
Spectrophotometer absorbance के आधार पर DNA amount और purity का अनुमान देता है। इसे quality check से जोड़ें।
B. जब DNA amount बहुत कम हो/When DNA amount is very low
Step 1
Concept
High-efficiency competent cells can be useful for low DNA amount or difficult ligation products.
Step 2
Why this answer is correct
The correct answer is B. जब DNA amount बहुत कम हो / When DNA amount is very low. High-efficiency competent cells can be useful for low DNA amount or difficult ligation products.
Step 3
Exam Tip
Low DNA amount या difficult ligation products के लिए high-efficiency competent cells useful हो सकती हैं।
