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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 401/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion के बाद blunt-end cloning में कौन सा factor अधिक critical हो सकता है?

Which factor can be more critical in blunt-end cloning after restriction digestion?

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A. DNA concentration और ligation conditionDNA concentration and ligation condition

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Blunt ends complementary overhang नहीं रखते। इसलिए concentration और ligation condition अधिक महत्वपूर्ण हो जाते हैं। / Blunt ends lack complementary overhang. Therefore concentration and ligation condition become more important.

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Question 402/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Sticky-end cloning में annealing किस कारण संभव होती है?

Why is annealing possible in sticky-end cloning?

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A. complementary overhangs के कारणBecause of complementary overhangs

Explanation

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Sticky ends complementary bases से temporary pair करते हैं। Ligase बाद में backbone जोड़ता है। / Sticky ends temporarily pair by complementary bases. Ligase later joins the backbone.

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Question 403/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion में ends compatible हों लेकिन ligation fail हो तो क्या कारण हो सकता है?

If ends are compatible but ligation fails in restriction digestion work what can be a cause?

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A. contaminants या wrong DNA ratioContaminants or wrong DNA ratio

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Compatible ends के अलावा clean DNA और सही ratio जरूरी है। Salt ethanol या enzyme carryover ligation रोक सकते हैं। / Besides compatible ends clean DNA and correct ratio are necessary. Salt ethanol or enzyme carryover can block ligation.

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Question 404/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion के बाद ligation setup में leftover restriction enzyme क्यों समस्या है?

Why is leftover restriction enzyme a problem in ligation setup after restriction digestion?

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A. वह DNA को फिर काट सकता हैIt can cut DNA again

Explanation

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Active restriction enzyme ligation mix में newly joined DNA को काट सकता है। Cleanup या inactivation useful है। / Active restriction enzyme can cut newly joined DNA in ligation mix. Cleanup or inactivation is useful.

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Question 405/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Heat inactivation के बाद भी cleanup कब बेहतर हो सकता है?

When can cleanup be better even after heat inactivation?

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A. जब buffer salts या enzymes next step affect करेंWhen buffer salts or enzymes affect next step

Explanation

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Heat inactivation enzyme रोक सकती है लेकिन salts और proteins रह सकते हैं। Cleanup next reaction को सुधार सकता है। / Heat inactivation can stop enzyme but salts and proteins may remain. Cleanup can improve next reaction.

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Question 406/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion के बाद PCR cleanup kit क्यों use हो सकती है?

Why can a PCR cleanup kit be used after restriction digestion?

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A. छोटे contaminants और buffer components हटाने के लिएTo remove small contaminants and buffer components

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Cleanup kit DNA को reaction components से अलग करती है। यह ligation या analysis से पहले useful है। / A cleanup kit separates DNA from reaction components. It is useful before ligation or analysis.

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Question 407/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Gel extraction और PCR cleanup में मुख्य अंतर क्या है?

What is the main difference between gel extraction and PCR cleanup?

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A. gel extraction specific band recover करती है और cleanup reaction mixture साफ करता हैGel extraction recovers specific band and cleanup cleans reaction mixture

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Gel extraction band selection देती है। Cleanup whole reaction DNA को impurities से साफ करता है। / Gel extraction gives band selection. Cleanup cleans whole reaction DNA from impurities.

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Question 408/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

अगर restriction digest में desired fragment unique नहीं है तो क्या करना सही हो सकता है?

If the desired fragment is not unique in restriction digest what can be appropriate?

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A. alternative enzymes या gel purification strategy चुननाChoosing alternative enzymes or gel purification strategy

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Non-unique fragments cloning को confuse कर सकते हैं। Better enzyme plan से clear fragment मिल सकता है। / Non-unique fragments can confuse cloning. A better enzyme plan can give a clear fragment.

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Question 409/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digest में fragment बहुत छोटा हो तो ligation में क्या issue हो सकता है?

If a fragment is very small in restriction digest what issue can occur in ligation?

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A. recovery और quantification कठिन हो सकती हैRecovery and quantification can be difficult

Explanation

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छोटे fragments gel extraction में खो सकते हैं। Accurate amount estimate भी कठिन हो सकता है। / Small fragments can be lost during gel extraction. Accurate amount estimation can also be difficult.

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Question 410/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digest में large fragment purification की चुनौती क्या हो सकती है?

What can be a challenge in large fragment purification after restriction digest?

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A. shearing और low recoveryShearing and low recovery

Explanation

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Large DNA fragments fragile हो सकते हैं। Gentle handling और suitable gel condition जरूरी है। / Large DNA fragments can be fragile. Gentle handling and suitable gel condition are needed.

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