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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 381/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digest reaction में enzyme को repeatedly thaw क्यों नहीं करना चाहिए?

Why should restriction enzyme not be thawed repeatedly?

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A. activity loss का risk बढ़ता हैRisk of activity loss increases

Explanation

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Repeated freeze-thaw enzyme protein को damage कर सकता है। Aliquot या quick handling useful है। / Repeated freeze-thaw can damage enzyme protein. Aliquot or quick handling is useful.

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Question 382/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction enzyme vial में contamination से क्या समस्या हो सकती है?

What problem can contamination in a restriction enzyme vial cause?

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A. many reactions fail हो सकती हैंMany reactions can fail

Explanation

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Contaminated enzyme stock multiple experiments खराब कर सकता है। Sterile tips और quick handling जरूरी हैं। / A contaminated enzyme stock can spoil multiple experiments. Sterile tips and quick handling are important.

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Question 383/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digestion में gel extraction के बाद concentration कम क्यों हो सकती है?

Why can concentration decrease after gel extraction in restriction digestion?

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A. recovery पूरी नहीं होतीRecovery is not complete

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Gel extraction में DNA loss हो सकता है। इसलिए final concentration फिर measure करना चाहिए। / DNA loss can occur in gel extraction. Therefore final concentration should be measured again.

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Question 384/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Gel extraction में silica column use हो तो DNA किससे bind कर सकता है?

If silica column is used in gel extraction what can DNA bind to?

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A. silica membrane

Explanation

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Suitable salt conditions में DNA silica membrane से bind करता है। Wash के बाद elution की जाती है। / Under suitable salt conditions DNA binds to silica membrane. Washing is followed by elution.

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Question 385/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Gel extraction में agarose melt करना क्यों जरूरी हो सकता है?

Why may agarose melting be necessary in gel extraction?

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A. DNA fragment को gel matrix से release करने के लिएTo release DNA fragment from gel matrix

Explanation

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Desired band agarose matrix में फंसा होता है। Melting और binding steps DNA recovery में मदद करते हैं। / The desired band is trapped in agarose matrix. Melting and binding steps help DNA recovery.

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Question 386/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Gel extraction में ज्यादा agarose slice लेने से क्या समस्या हो सकती है?

What problem can occur if too much agarose slice is taken in gel extraction?

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A. purification efficiency घट सकती हैPurification efficiency can decrease

Explanation

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बड़ा gel slice impurities और volume बढ़ाता है। केवल desired band के आसपास का छोटा slice लें। / A large gel slice increases impurities and volume. Take only a small slice around the desired band.

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Question 387/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Gel extraction में close band contamination से क्या होगा?

What happens due to close band contamination in gel extraction?

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A. wrong fragment साथ आ सकता हैWrong fragment can come along

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पास की band accidentally cut होने पर unwanted DNA आ सकता है। High resolution separation helpful है। / If a nearby band is accidentally cut unwanted DNA can come. High resolution separation is helpful.

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Question 388/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digestion experiment में conclusion लिखते समय क्या avoid करना चाहिए?

What should be avoided while writing conclusion of restriction digestion experiment?

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A. बिना control के निश्चित दावा करनाMaking a definite claim without controls

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Controls के बिना result interpretation कमजोर होता है। Scientific conclusion evidence पर आधारित होना चाहिए। / Without controls result interpretation is weak. Scientific conclusion should be based on evidence.

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Question 389/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digest में ethical data handling का एक नियम क्या है?

What is one rule of ethical data handling in restriction digest?

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A. original gel image सुरक्षित रखेंPreserve original gel image

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Original image और lane map transparency देते हैं। Result को manipulate नहीं करना चाहिए। / Original image and lane map give transparency. Results should not be manipulated.

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Question 390/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digestion में biosafety record क्यों useful है?

Why is a biosafety record useful in restriction digestion?

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A. used stains chemicals और waste tracking के लिएFor tracking used stains chemicals and waste

Explanation

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Stains और chemicals का disposal record safety compliance में मदद करता है। Lab safety भी experiment का भाग है। / Disposal record of stains and chemicals helps safety compliance. Lab safety is also part of experiment.

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