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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 391/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Level 52 का best revision point क्या है?

What is the best revision point of Level 52?

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A. quality control expected observed bands partial digest star activity और cleanup याद रखेंRemember quality control expected observed bands partial digest star activity and cleanup

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Restriction digestion result को map controls और gel pattern से verify करें। Troubleshooting में DNA quality और enzyme conditions check करें। / Verify restriction digestion result using map controls and gel pattern. In troubleshooting check DNA quality and enzyme conditions.

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Question 392/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Cutting of DNA level 52 का final summary क्या है?

What is the final summary of Cutting of DNA level 52?

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A. reliable digestion के लिए correct enzyme condition controls gel interpretation और records जरूरी हैंCorrect enzyme condition controls gel interpretation and records are needed for reliable digestion

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DNA cutting में केवल band देखना काफी नहीं है। Expected pattern controls और cleanup भी important हैं। / Only seeing a band is not enough in DNA cutting. Expected pattern controls and cleanup are also important.

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Question 393/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion के बाद ligation के लिए सबसे जरूरी DNA feature क्या है?

What is the most important DNA feature for ligation after restriction digestion?

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A. compatible clean ends

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Compatible clean ends ligase को joining में मदद करते हैं। Impurities ligation को रोक सकती हैं। / Compatible clean ends help ligase in joining. Impurities can block ligation.

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Question 394/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction cut vector को ligation से पहले purify क्यों करते हैं?

Why is a restriction-cut vector purified before ligation?

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A. buffer enzyme और small fragments हटाने के लिएTo remove buffer enzyme and small fragments

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Purification ligation inhibitors कम करती है। Clean vector ends recombinant DNA formation में मदद करते हैं। / Purification reduces ligation inhibitors. Clean vector ends help recombinant DNA formation.

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Question 395/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction cut insert को purify करने का मुख्य कारण क्या है?

What is the main reason for purifying a restriction-cut insert?

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A. desired fragment को unwanted DNA से अलग करनाTo separate desired fragment from unwanted DNA

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Insert purification unwanted fragments और salts हटाती है। इससे ligation specificity बेहतर होती है। / Insert purification removes unwanted fragments and salts. This improves ligation specificity.

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Question 396/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion के बाद insert और vector ratio क्यों set किया जाता है?

Why is insert and vector ratio set after restriction digestion?

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A. recombinant ligation chance बढ़ाने के लिएTo increase chance of recombinant ligation

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सही insert-vector ratio recombinant clones बढ़ा सकता है। बहुत गलत ratio cloning efficiency घटा सकता है। / Correct insert-vector ratio can increase recombinant clones. A very wrong ratio can reduce cloning efficiency.

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Question 397/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Vector self-ligation का अर्थ क्या है?

What does vector self-ligation mean?

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A. vector ends का insert बिना आपस में जुड़नाVector ends joining each other without insert

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Self-ligation empty vector colonies दे सकती है। Dephosphorylation या directional cloning इसे घटाती है। / Self-ligation can give empty vector colonies. Dephosphorylation or directional cloning reduces it.

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Question 398/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction cut vector में dephosphorylation क्यों की जाती है?

Why is dephosphorylation done in a restriction-cut vector?

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A. self-ligation कम करने के लिएTo reduce self-ligation

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Phosphate हटाने से vector ends का self-joining कम हो सकता है। इससे insert-containing clones की संभावना बढ़ती है। / Removing phosphate can reduce self-joining of vector ends. This increases chance of insert-containing clones.

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Question 399/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Dephosphorylated vector को ligate करने के लिए insert में क्या होना चाहिए?

For ligating a dephosphorylated vector what should the insert have?

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A. phosphate group

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Ligase phosphodiester bond बनाने के लिए phosphate की जरूरत रखता है। Insert phosphate joining में मदद करता है। / Ligase needs phosphate to form phosphodiester bond. Insert phosphate helps joining.

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Question 400/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction cut ends पर phosphate क्यों महत्वपूर्ण है?

Why is phosphate important at restriction-cut ends?

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A. ligase द्वारा bond formation के लिएFor bond formation by ligase

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DNA ligase phosphodiester bond बनाता है। इसके लिए सही chemical ends जरूरी हैं। / DNA ligase forms phosphodiester bonds. Correct chemical ends are needed for this.

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