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Which enzymes are mainly used for cutting DNA?
A. प्रतिबंध एंजाइमRestriction enzymes
प्रतिबंध एंजाइम डीएनए को खास स्थानों पर काटते हैं। परीक्षा में इन्हें molecular scissors याद रखें। / Restriction enzymes cut DNA at specific sites. In exams remember them as molecular scissors.
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What is an enzyme that cuts DNA at a specific site called?
B. प्रतिबंध एंडोन्यूक्लिएजRestriction endonuclease
Restriction endonuclease डीएनए के अंदर specific sequence पर cut लगाता है। यह recombinant DNA technology में जरूरी है। / A restriction endonuclease cuts inside DNA at a specific sequence. It is essential in recombinant DNA technology.
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Which bond is cut by restriction enzymes in DNA?
C. फॉस्फोडाइएस्टर bondPhosphodiester bond
Restriction enzymes DNA backbone के phosphodiester bonds को काटते हैं। इससे DNA fragments बनते हैं। / Restriction enzymes cut phosphodiester bonds in the DNA backbone. This forms DNA fragments.
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What does recognition site mean for a restriction enzyme?
D. डीएनए की वह sequence जिसे enzyme पहचानता हैDNA sequence recognized by the enzyme
Recognition site वह specific DNA sequence है जहाँ enzyme bind और cut करता है। हर enzyme का site अलग हो सकता है। / A recognition site is the specific DNA sequence where an enzyme binds and cuts. Each enzyme may have a different site.
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What is the correct meaning of a palindromic sequence?
A. दोनों strands पर opposite दिशा में समान पढ़ी जाने वाली sequenceA sequence read the same on both strands in opposite directions
कई restriction enzymes palindromic sequences पहचानते हैं। इसे दोनों strands की symmetry से याद रखें। / Many restriction enzymes recognize palindromic sequences. Remember it by symmetry of both strands.
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What type of ends are generally produced by EcoRI cutting?
C. sticky ends
EcoRI staggered cut लगाकर sticky ends बनाता है। Sticky ends ligation में मदद कर सकते हैं। / EcoRI makes staggered cuts producing sticky ends. Sticky ends can help in ligation.
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D. बिना overhang वाला सीधा DNA endStraight DNA end without overhang
Blunt ends में कोई single-stranded overhang नहीं होता। ये भी ligate हो सकते हैं पर sticky ends की तुलना में कठिन हो सकते हैं। / Blunt ends have no single-stranded overhang. They can ligate but may be harder than sticky ends.
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What is the main advantage of a sticky end?
A. complementary ends के साथ pairing आसान होती हैPairing with complementary ends becomes easier
Sticky ends complementary bases से pair कर सकते हैं। इसलिए recombinant DNA बनाने में ये उपयोगी हैं। / Sticky ends can pair by complementary bases. Therefore they are useful in making recombinant DNA.
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What is formed by a staggered cut?
B. sticky ends with overhangs
Staggered cut DNA strands को अलग positions पर काटता है। इससे overhang वाले sticky ends बनते हैं। / A staggered cut cuts DNA strands at different positions. This forms sticky ends with overhangs.
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What type of DNA ends are produced by a straight cut?
C. blunt ends
Straight cut दोनों strands को same position पर काटता है। इससे blunt ends बनते हैं। / A straight cut cuts both strands at the same position. This produces blunt ends.
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