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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 21/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 45

एक restriction enzyme के recognition site न होने पर क्या होगा?

What happens if a DNA molecule lacks the recognition site of a restriction enzyme?

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A. DNA उसी enzyme से specific तरीके से नहीं कटेगाDNA will not be specifically cut by that enzyme

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Restriction enzyme अपने recognition site के बिना cut नहीं कर सकता। इसलिए sequence information important है। / A restriction enzyme cannot cut without its recognition site. Therefore sequence information is important.

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Question 22/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 45

एक DNA molecule में enzyme site दो बार हो तो digestion के बाद क्या हो सकता है?

If an enzyme site occurs twice in a DNA molecule what can happen after digestion?

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C. तीन fragments बन सकते हैं if linear DNAThree fragments can form if DNA is linear

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Linear DNA में दो cut sites होने पर तीन fragments बन सकते हैं। Fragment number site position पर निर्भर करता है। / In linear DNA two cut sites can produce three fragments. Fragment number depends on site positions.

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Question 23/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 45

Circular plasmid पर एक restriction site हो तो digestion से क्या बनता है?

If a circular plasmid has one restriction site what is formed after digestion?

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D. linear plasmid

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Circular plasmid एक site पर कटकर linear molecule बन सकता है। Gel पर supercoiled और linear forms अलग दिख सकते हैं। / A circular plasmid cut at one site can become a linear molecule. Supercoiled and linear forms can appear differently on gel.

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Question 24/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 45

Circular plasmid पर दो अलग cut sites हों तो क्या बन सकता है?

What can form if a circular plasmid has two different cut sites?

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A. दो DNA fragmentsTwo DNA fragments

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Circular DNA पर दो cuts generally दो fragments देते हैं। यह insert release या diagnostic digestion में उपयोगी है। / Two cuts on circular DNA generally give two fragments. This is useful for insert release or diagnostic digestion.

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Question 25/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 45

Restriction digestion result देखने के लिए DNA ladder क्यों लगाई जाती है?

Why is a DNA ladder loaded to view restriction digestion results?

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B. fragment size estimate करने के लिएTo estimate fragment size

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DNA ladder known size fragments देती है। इससे sample fragments का size estimate होता है। / A DNA ladder provides fragments of known sizes. This helps estimate sample fragment sizes.

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Question 26/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 45

Restriction digestion के बाद gel पर band क्यों दिखते हैं?

Why do bands appear on a gel after restriction digestion?

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A. DNA fragments size के अनुसार अलग होते हैंDNA fragments separate according to size

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Gel electrophoresis DNA fragments को size के अनुसार अलग करता है। Bands digestion pattern दिखाते हैं। / Gel electrophoresis separates DNA fragments by size. Bands show the digestion pattern.

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Question 27/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 45

Small DNA fragments gel में सामान्यतः कैसे move करते हैं?

How do small DNA fragments generally move in a gel?

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B. बड़े fragments से अधिक दूरीMore distance than large fragments

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छोटे fragments agarose gel में faster और farther move करते हैं। यह size separation का आधार है। / Small fragments move faster and farther in agarose gel. This is the basis of size separation.

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Question 28/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 45

डीएनए cutting से पहले DNA purity क्यों important है?

Why is DNA purity important before DNA cutting?

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A. impurities enzyme को inhibit कर सकती हैंImpurities can inhibit the enzyme

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Phenol salt या ethanol जैसे contaminants enzyme activity घटा सकते हैं। Clean DNA digestion के लिए बेहतर है। / Contaminants such as phenol salt or ethanol can reduce enzyme activity. Clean DNA is better for digestion.

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Question 29/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 45

Restriction digestion में DNA methylation क्या प्रभाव डाल सकती है?

What effect can DNA methylation have in restriction digestion?

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A. enzyme recognition या cutting को प्रभावित कर सकती हैIt can affect enzyme recognition or cutting

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कुछ restriction enzymes methylated sites नहीं काटते। इसलिए methylation status digestion result बदल सकता है। / Some restriction enzymes do not cut methylated sites. Therefore methylation status can change digestion results.

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Question 30/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 45

Bacteria अपने DNA को restriction enzyme से कैसे बचा सकते हैं?

How can bacteria protect their own DNA from restriction enzymes?

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B. methylation द्वाराBy methylation

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Bacteria अपने recognition sites को methylate कर सकते हैं। इससे own DNA restriction enzyme से सुरक्षित रहता है। / Bacteria can methylate their recognition sites. This protects their own DNA from restriction enzymes.

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