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9 questions tagged with enzyme inhibition.
What can be the effect of phenol carryover in restriction digest?
A. enzyme inhibition
Phenol enzyme proteins को affect कर सकता है। Clean DNA digestion के लिए जरूरी है। / Phenol can affect enzyme proteins. Clean DNA is necessary for digestion.
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What happens if EDTA is high in restriction enzyme reaction?
A. magnesium chelation से enzyme activity घट सकती हैEnzyme activity can decrease due to magnesium chelation
EDTA magnesium ions bind करता है। कई restriction enzymes magnesium dependent होते हैं। / EDTA binds magnesium ions. Many restriction enzymes are magnesium dependent.
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Which DNA purity issue can be very problematic for restriction enzyme reaction?
A. phenol ethanol या salt carryoverPhenol ethanol or salt carryover
Phenol ethanol और salts enzyme activity inhibit कर सकते हैं। Digestion से पहले cleanup useful हो सकता है। / Phenol ethanol and salts can inhibit enzyme activity. Cleanup can be useful before digestion.
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Why is a clean DNA sample necessary in restriction digest?
A. restriction enzyme impurities से inhibit हो सकता हैRestriction enzyme can be inhibited by impurities
Phenol ethanol और salts enzyme activity कम कर सकते हैं। इसलिए digestion से पहले DNA purity check करें। / Phenol ethanol and salts can reduce enzyme activity. Therefore check DNA purity before digestion.
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Why should leftover ethanol not be present in a DNA sample for restriction digestion?
A. ethanol enzyme reaction inhibit कर सकता हैEthanol can inhibit enzyme reaction
Residual ethanol restriction enzyme activity घटा सकता है। DNA pellet को सही तरह dry करना चाहिए। / Residual ethanol can reduce restriction enzyme activity. The DNA pellet should be dried properly.
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Why is DNA purity important before DNA cutting?
A. impurities enzyme को inhibit कर सकती हैंImpurities can inhibit the enzyme
Phenol salt या ethanol जैसे contaminants enzyme activity घटा सकते हैं। Clean DNA digestion के लिए बेहतर है। / Contaminants such as phenol salt or ethanol can reduce enzyme activity. Clean DNA is better for digestion.
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What harm can leftover phenol cause in a DNA sample?
B. enzyme reactions inhibit हो सकती हैंEnzyme reactions can be inhibited
Phenol contamination PCR और restriction digestion जैसी reactions को प्रभावित कर सकती है। Proper cleanup जरूरी है। / Phenol contamination can affect reactions like PCR and restriction digestion. Proper cleanup is necessary.
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What effect can salt carryover have in a DNA sample?
A. downstream enzyme reactions प्रभावित हो सकती हैंDownstream enzyme reactions can be affected
Excess salt PCR या enzyme digestion को inhibit कर सकता है। Wash step salt removal में मदद करता है। / Excess salt can inhibit PCR or enzyme digestion. Wash step helps salt removal.
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What problem can occur if phenol remains in a DNA sample?
A. enzyme reactions inhibit हो सकती हैंEnzyme reactions can be inhibited
Phenol contamination PCR और restriction digestion जैसी reactions को affect कर सकती है। Proper purification जरूरी है। / Phenol contamination can affect reactions like PCR and restriction digestion. Proper purification is necessary.
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