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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 411/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Large DNA fragments को protect करने के लिए क्या practice अच्छी है?

What practice is good to protect large DNA fragments?

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A. gentle handling और low UV exposureGentle handling and low UV exposure

Explanation

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Large fragments rough handling और UV से damage हो सकते हैं। Gentle handling recovery बचाती है। / Large fragments can be damaged by rough handling and UV. Gentle handling preserves recovery.

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Question 412/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion के बाद vector band को gel से काटते समय क्या सावधानी जरूरी है?

What precaution is needed while cutting vector band from gel after restriction digestion?

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A. पास की unwanted band से बचनाAvoiding nearby unwanted band

Explanation

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गलत band काटने से wrong DNA purify हो सकता है। Band identity ladder और map से confirm करें। / Cutting the wrong band can purify wrong DNA. Confirm band identity with ladder and map.

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Question 413/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion में band excision से पहले क्या compare करना चाहिए?

What should be compared before band excision in restriction digestion?

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A. band size with ladder and expected map

Explanation

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Band excision से पहले expected size और ladder से match करें। इससे wrong band purification से बचते हैं। / Before band excision match expected size with ladder. This prevents wrong band purification.

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Question 414/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion के बाद purified DNA को फिर gel पर क्यों check किया जा सकता है?

Why can purified DNA be checked again on gel after restriction digestion?

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A. purity size और recovery देखने के लिएTo see purity size and recovery

Explanation

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Post-purification gel confirm करता है कि desired fragment recovered है। यह ligation से पहले useful check है। / A post-purification gel confirms that desired fragment is recovered. It is useful before ligation.

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Question 415/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digest में purified fragment में salt बचा हो तो ligation पर क्या असर होगा?

What effect can leftover salt in purified fragment have on ligation?

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A. ligation efficiency घट सकती हैLigation efficiency can decrease

Explanation

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Salt carryover ligase reaction को प्रभावित कर सकता है। Final wash और drying सही करें। / Salt carryover can affect ligase reaction. Do final wash and drying properly.

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Question 416/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digest purification में ethanol carryover क्यों avoid करना चाहिए?

Why should ethanol carryover be avoided in restriction digest purification?

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A. ligation और enzyme reactions inhibit हो सकती हैंLigation and enzyme reactions can be inhibited

Explanation

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Residual ethanol downstream reactions को रोक सकता है। Purified DNA को सही तरह dry करें। / Residual ethanol can inhibit downstream reactions. Dry purified DNA properly.

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Question 417/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion से मिली DNA concentration गलत estimate हो तो क्या समस्या हो सकती है?

What problem can occur if DNA concentration from restriction digestion is estimated wrongly?

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A. ligation ratio गलत हो सकता हैLigation ratio can be wrong

Explanation

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Insert-vector ratio concentration पर आधारित होता है। गलत estimate cloning efficiency घटा सकता है। / Insert-vector ratio is based on concentration. Wrong estimate can reduce cloning efficiency.

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Question 418/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion में molar ratio mass ratio से क्यों अलग हो सकता है?

Why can molar ratio differ from mass ratio in restriction digestion work?

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A. fragment length अलग होने के कारणBecause fragment lengths differ

Explanation

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लंबे और छोटे fragments की same mass में molecule numbers अलग होते हैं। Ligation में molar ratio important है। / Long and short fragments have different molecule numbers at the same mass. Molar ratio is important in ligation.

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Question 419/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Insert बहुत अधिक हो तो ligation result पर क्या असर हो सकता है?

What effect can too much insert have on ligation result?

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A. multiple inserts या inefficient cloning riskRisk of multiple inserts or inefficient cloning

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Excess insert abnormal ligation products बढ़ा सकता है। Suitable ratio use करें। / Excess insert can increase abnormal ligation products. Use a suitable ratio.

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Question 420/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Vector बहुत अधिक हो और insert कम हो तो क्या risk है?

What is the risk if vector is too much and insert is low?

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A. empty vector colonies बढ़ सकती हैंEmpty vector colonies can increase

Explanation

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Low insert availability vector self-ligation या empty colonies का chance बढ़ा सकती है। Ratio optimize करें। / Low insert availability can increase chance of vector self-ligation or empty colonies. Optimize ratio.

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