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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 431/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digest के बाद छोटे कटे हुए adapter fragments क्यों हटाने चाहिए?

Why should small cut adapter fragments be removed after restriction digest?

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A. वे ligation reaction में unwanted competition दे सकते हैंThey can give unwanted competition in ligation reaction

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Small fragments compatible ends रख सकते हैं और ligation में confuse कर सकते हैं। Cleanup इन्हें हटाता है। / Small fragments can have compatible ends and confuse ligation. Cleanup removes them.

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Question 432/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion में purification के बाद elution buffer choose करते समय क्या ध्यान रखें?

What should be considered while choosing elution buffer after purification in restriction digestion?

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A. next ligation या enzyme reaction compatibilityCompatibility with next ligation or enzyme reaction

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Elution buffer में ऐसे components न हों जो ligation inhibit करें। Downstream use के अनुसार buffer चुनें। / Elution buffer should not contain components that inhibit ligation. Choose buffer according to downstream use.

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Question 433/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digest purification में nuclease-free water कब useful हो सकता है?

When can nuclease-free water be useful in restriction digest purification?

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A. जब downstream enzyme reaction sensitive होWhen downstream enzyme reaction is sensitive

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Nuclease-free water unwanted nucleases से बचाता है और कई downstream reactions में compatible होता है। / Nuclease-free water avoids unwanted nucleases and is compatible with many downstream reactions.

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Question 434/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digest से पहले DNA sample का A260 by A280 ratio क्यों देखा जा सकता है?

Why can A260 by A280 ratio of DNA sample be checked before restriction digest?

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A. protein contamination का अनुमान लगाने के लिएTo estimate protein contamination

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Protein contamination enzyme activity को affect कर सकती है। Ratio DNA purity check में मदद करता है। / Protein contamination can affect enzyme activity. The ratio helps check DNA purity.

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Question 435/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

A260 by A230 ratio restriction digest से पहले क्यों useful हो सकता है?

Why can A260 by A230 ratio be useful before restriction digest?

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A. salt phenol carbohydrate contamination detect करने के लिएTo detect salt phenol carbohydrate contamination

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A260 by A230 खराब होने पर contaminants enzyme reactions रोक सकते हैं। Cleanup पर विचार करें। / If A260 by A230 is poor contaminants can inhibit enzyme reactions. Consider cleanup.

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Question 436/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digest में DNA integrity क्यों जरूरी है?

Why is DNA integrity important in restriction digest?

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A. broken DNA expected pattern बदल सकता हैBroken DNA can change expected pattern

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Degraded DNA extra smear या missing bands दे सकता है। Integrity gel check से समझें। / Degraded DNA can give extra smear or missing bands. Understand integrity by gel check.

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Question 437/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digest से पहले genomic DNA बहुत degraded हो तो result कैसा हो सकता है?

If genomic DNA is highly degraded before restriction digest how can result look?

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A. smear और unclear fragmentsSmear and unclear fragments

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Degraded DNA already छोटे fragments में टूट चुका होता है। Restriction pattern clear नहीं मिल सकता। / Degraded DNA is already broken into small fragments. Restriction pattern may not be clear.

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Question 438/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion में high-quality plasmid prep क्यों जरूरी है?

Why is high-quality plasmid prep necessary in restriction digestion?

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A. clear and reliable band pattern पाने के लिएTo obtain clear and reliable band pattern

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Clean plasmid enzyme digestion में better काम करता है। Contaminants partial digestion दे सकते हैं। / Clean plasmid works better in enzyme digestion. Contaminants can give partial digestion.

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Question 439/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion के बाद next transformation step से पहले recombinant DNA कैसे बनता है?

How is recombinant DNA formed before the next transformation step after restriction digestion?

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A. cut vector और insert को ligase से जोड़करBy joining cut vector and insert with ligase

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Restriction digestion compatible ends बनाता है। DNA ligase इन्हें जोड़कर recombinant DNA बनाता है। / Restriction digestion creates compatible ends. DNA ligase joins them to make recombinant DNA.

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Question 440/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Cutting of DNA step cloning workflow में कहाँ आता है?

Where does Cutting of DNA step come in cloning workflow?

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A. DNA isolation के बाद और ligation से पहलेAfter DNA isolation and before ligation

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पहले DNA isolate किया जाता है फिर restriction cutting होती है। उसके बाद ligation और transformation आते हैं। / First DNA is isolated then restriction cutting occurs. After that come ligation and transformation.

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