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ligase MCQ Questions for Class 11

ligase se related questions ko ek jagah revise karein. Har question me bilingual content, answer feedback aur explanation available hai.

Practice Questions

71 questions tagged with ligase.

Restriction digestion के बाद next transformation step से पहले recombinant DNA कैसे बनता है?

How is recombinant DNA formed before the next transformation step after restriction digestion?

Explanation opens after your attempt
Correct Answer

A. cut vector और insert को ligase से जोड़करBy joining cut vector and insert with ligase

Step 1

Concept

Restriction digestion creates compatible ends. DNA ligase joins them to make recombinant DNA.

Step 2

Why this answer is correct

The correct answer is A. cut vector और insert को ligase से जोड़कर / By joining cut vector and insert with ligase. Restriction digestion creates compatible ends. DNA ligase joins them to make recombinant DNA.

Step 3

Exam Tip

Restriction digestion compatible ends बनाता है। DNA ligase इन्हें जोड़कर recombinant DNA बनाता है।

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Restriction digestion से बने ends को ligase स्थायी कैसे बनाता है?

How does ligase make ends produced by restriction digestion permanent?

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Correct Answer

A. phosphodiester bonds बनाकरBy forming phosphodiester bonds

Step 1

Concept

Ligase forms phosphodiester bonds in the DNA backbone. This makes recombinant DNA stable.

Step 2

Why this answer is correct

The correct answer is A. phosphodiester bonds बनाकर / By forming phosphodiester bonds. Ligase forms phosphodiester bonds in the DNA backbone. This makes recombinant DNA stable.

Step 3

Exam Tip

Ligase DNA backbone में phosphodiester bonds बनाता है। इससे recombinant DNA स्थायी बनता है।

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कौन सा option ligase enzymes को Class 11 context में best summarize करता है?

Which option best summarizes ligase enzymes in Class 11 context?

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Correct Answer

A. DNA ends जोड़ने वाले enzymes useful in cloning and replicationDNA-end joining enzymes useful in cloning and replication

Step 1

Concept

In Class 11 link ligase with DNA joining in cloning and Okazaki fragment joining in replication. This is the safest summary.

Step 2

Why this answer is correct

The correct answer is A. DNA ends जोड़ने वाले enzymes useful in cloning and replication / DNA-end joining enzymes useful in cloning and replication. In Class 11 link ligase with DNA joining in cloning and Okazaki fragment joining in replication. This is the safest summary.

Step 3

Exam Tip

Class 11 में ligase को cloning में DNA joining और replication में Okazaki fragment joining से जोड़ें। यही safest summary है।

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कौन सा option ligase के लिए सही नहीं है?

Which option is not correct for ligase?

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Correct Answer

D. It works as main starch digestive enzyme

Step 1

Concept

Ligase is not a starch digestive enzyme. It is an enzyme that joins DNA ends.

Step 2

Why this answer is correct

The correct answer is D. It works as main starch digestive enzyme. Ligase is not a starch digestive enzyme. It is an enzyme that joins DNA ends.

Step 3

Exam Tip

Ligase starch digestion enzyme नहीं है। यह DNA ends को जोड़ने वाला enzyme है।

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किस option में ligase enzyme की सबसे basic पहचान है?

Which option gives the most basic identity of ligase enzyme?

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Correct Answer

A. DNA joining enzyme

Step 1

Concept

The basic identity of ligase is DNA joining enzyme. Do not confuse it with restriction enzyme.

Step 2

Why this answer is correct

The correct answer is A. DNA joining enzyme. The basic identity of ligase is DNA joining enzyme. Do not confuse it with restriction enzyme.

Step 3

Exam Tip

Ligase की basic पहचान DNA joining enzyme है। इसे restriction enzyme से confuse न करें।

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Ligase enzyme किस broad enzyme class की idea से मेल खाता है?

Ligase enzyme matches the idea of which broad enzyme class?

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A. molecules को जोड़ने वाले enzymesEnzymes that join molecules

Step 1

Concept

Ligases are enzymes that join molecules using energy. DNA ligase joins DNA ends.

Step 2

Why this answer is correct

The correct answer is A. molecules को जोड़ने वाले enzymes / Enzymes that join molecules. Ligases are enzymes that join molecules using energy. DNA ligase joins DNA ends.

Step 3

Exam Tip

Ligases ऊर्जा की मदद से molecules को जोड़ने वाले enzymes हैं। DNA ligase DNA ends जोड़ता है।

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किस option में ligase और nick का correct relation है?

Which option correctly relates ligase and nick?

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Correct Answer

A. ligase nick seal करता हैLigase seals the nick

Step 1

Concept

A nick is a small break in DNA backbone. Ligase seals it and restores continuity.

Step 2

Why this answer is correct

The correct answer is A. ligase nick seal करता है / Ligase seals the nick. A nick is a small break in DNA backbone. Ligase seals it and restores continuity.

Step 3

Exam Tip

Nick DNA backbone में छोटा टूटाव है। Ligase इसे seal करके continuity restore करता है।

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Ligase किस तरह DNA continuity बनाता है?

How does ligase maintain DNA continuity?

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Correct Answer

A. backbone nick seal करकेBy sealing backbone nick

Step 1

Concept

Ligase seals nicks in the DNA backbone. This makes the DNA strand continuous.

Step 2

Why this answer is correct

The correct answer is A. backbone nick seal करके / By sealing backbone nick. Ligase seals nicks in the DNA backbone. This makes the DNA strand continuous.

Step 3

Exam Tip

Ligase DNA backbone में निक को seal करता है। इससे DNA strand continuous बनता है।

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कौन सा option ligase और DNA repair की सही बात है?

Which option is correct about ligase and DNA repair?

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Correct Answer

A. ligase DNA strand में break seal कर सकता हैLigase can seal a break in DNA strand

Step 1

Concept

In DNA repair ligase can help seal a strand nick or break. This maintains genome stability.

Step 2

Why this answer is correct

The correct answer is A. ligase DNA strand में break seal कर सकता है / Ligase can seal a break in DNA strand. In DNA repair ligase can help seal a strand nick or break. This maintains genome stability.

Step 3

Exam Tip

DNA repair में ligase strand nick या break को seal करने में मदद कर सकता है। इससे genome stability बनी रहती है।

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Ligase reaction में बहुत अधिक heat का क्या असर हो सकता है?

What can be the effect of too much heat in ligase reaction?

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Correct Answer

A. ligase denature होकर inactive हो सकता हैLigase can denature and become inactive

Step 1

Concept

Ligase is a protein enzyme and can denature at high heat. This can reduce ligation efficiency.

Step 2

Why this answer is correct

The correct answer is A. ligase denature होकर inactive हो सकता है / Ligase can denature and become inactive. Ligase is a protein enzyme and can denature at high heat. This can reduce ligation efficiency.

Step 3

Exam Tip

Ligase protein enzyme है और अधिक heat से denature हो सकता है। इससे ligation efficiency घट सकती है।

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Ligase enzyme को cold condition में रखने का कारण क्या है?

Why is ligase enzyme kept in cold condition?

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Correct Answer

A. enzyme activity preserve करने के लिएTo preserve enzyme activity

Step 1

Concept

Ligase is a protein enzyme and can become inactive by heat. Cold condition helps preserve activity.

Step 2

Why this answer is correct

The correct answer is A. enzyme activity preserve करने के लिए / To preserve enzyme activity. Ligase is a protein enzyme and can become inactive by heat. Cold condition helps preserve activity.

Step 3

Exam Tip

Ligase protein enzyme है और heat से inactive हो सकता है। Cold condition activity बचाने में मदद करती है।

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कौन सा factor ligation efficiency को प्रभावित कर सकता है?

Which factor can affect ligation efficiency?

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Correct Answer

A. DNA end compatibility

Step 1

Concept

If DNA ends are compatible ligation can be easier. Incompatible ends can make joining difficult.

Step 2

Why this answer is correct

The correct answer is A. DNA end compatibility. If DNA ends are compatible ligation can be easier. Incompatible ends can make joining difficult.

Step 3

Exam Tip

DNA ends compatible हों तो ligation आसान हो सकती है। Incompatible ends joining को कठिन बना सकते हैं।

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Directional cloning में ligase किस चीज को जोड़ता है?

In directional cloning what does ligase join?

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Correct Answer

A. compatible designed ends of insert and vector

Step 1

Concept

In directional cloning different ends are designed. Ligase joins compatible insert and vector ends.

Step 2

Why this answer is correct

The correct answer is A. compatible designed ends of insert and vector. In directional cloning different ends are designed. Ligase joins compatible insert and vector ends.

Step 3

Exam Tip

Directional cloning में अलग ends design किए जाते हैं। Ligase compatible insert और vector ends को जोड़ता है।

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डीएनए ligase किस प्रकार के bond को repair या create करता है?

Which type of bond does DNA ligase repair or create?

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Correct Answer

C. phosphodiester bond in DNA

Step 1

Concept

DNA ligase repairs or creates phosphodiester bonds in the DNA backbone. It is needed for DNA continuity.

Step 2

Why this answer is correct

The correct answer is C. phosphodiester bond in DNA. DNA ligase repairs or creates phosphodiester bonds in the DNA backbone. It is needed for DNA continuity.

Step 3

Exam Tip

DNA ligase DNA backbone में phosphodiester bond repair या create करता है। यह DNA continuity के लिए जरूरी है।

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किससे recombinant plasmid स्थिर बनता है?

What makes a recombinant plasmid stable?

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Correct Answer

A. vector और insert के बीच phosphodiester bondPhosphodiester bond between vector and insert

Step 1

Concept

Ligase forms a phosphodiester bond between vector and insert. This makes the recombinant plasmid stable.

Step 2

Why this answer is correct

The correct answer is A. vector और insert के बीच phosphodiester bond / Phosphodiester bond between vector and insert. Ligase forms a phosphodiester bond between vector and insert. This makes the recombinant plasmid stable.

Step 3

Exam Tip

Ligase vector और insert के बीच phosphodiester bond बनाता है। यही recombinant plasmid को stable बनाता है।

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लाइगेज किस step में उपयोगी है?

In which step is ligase useful?

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Correct Answer

D. DNA fragments को जोड़नाJoining DNA fragments

Step 1

Concept

Ligase joins DNA fragments. DNA cutting is done by restriction enzyme.

Step 2

Why this answer is correct

The correct answer is D. DNA fragments को जोड़ना / Joining DNA fragments. Ligase joins DNA fragments. DNA cutting is done by restriction enzyme.

Step 3

Exam Tip

Ligase DNA fragments को जोड़ता है। DNA cutting restriction enzyme करता है।

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Ligase enzyme का correct analogy क्या है?

What is the correct analogy for ligase enzyme?

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Correct Answer

A. glue

Step 1

Concept

Ligase joins DNA fragments so the glue analogy is correct. The scissors analogy is for restriction enzymes.

Step 2

Why this answer is correct

The correct answer is A. glue. Ligase joins DNA fragments so the glue analogy is correct. The scissors analogy is for restriction enzymes.

Step 3

Exam Tip

Ligase DNA fragments को जोड़ता है इसलिए glue analogy सही है। Scissors analogy restriction enzymes के लिए है।

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Ligase किस process में vector और insert को covalently जोड़ता है?

In which process does ligase covalently join vector and insert?

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Correct Answer

A. DNA cloning

Step 1

Concept

In DNA cloning ligase forms a covalent phosphodiester bond between vector and insert. This makes a stable recombinant plasmid.

Step 2

Why this answer is correct

The correct answer is A. DNA cloning. In DNA cloning ligase forms a covalent phosphodiester bond between vector and insert. This makes a stable recombinant plasmid.

Step 3

Exam Tip

DNA cloning में ligase vector और insert के बीच covalent phosphodiester bond बनाता है। इससे stable recombinant plasmid बनता है।

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कौन सा option ligase के लिए exam-oriented पहचान है?

Which option is an exam-oriented identity for ligase?

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Correct Answer

A. DNA joining enzyme

Step 1

Concept

The most important identity of ligase is DNA joining enzyme. Keep it separate from restriction enzyme.

Step 2

Why this answer is correct

The correct answer is A. DNA joining enzyme. The most important identity of ligase is DNA joining enzyme. Keep it separate from restriction enzyme.

Step 3

Exam Tip

Ligase की सबसे important पहचान DNA joining enzyme है। इसे restriction enzyme से अलग रखें।

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Ligase को recombinant DNA technology में essential क्यों माना जाता है?

Why is ligase considered essential in recombinant DNA technology?

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Correct Answer

A. क्योंकि यह vector और insert को स्थायी रूप से जोड़ता हैBecause it permanently joins vector and insert

Step 1

Concept

To make recombinant DNA different DNA fragments must be joined. Ligase performs this joining.

Step 2

Why this answer is correct

The correct answer is A. क्योंकि यह vector और insert को स्थायी रूप से जोड़ता है / Because it permanently joins vector and insert. To make recombinant DNA different DNA fragments must be joined. Ligase performs this joining.

Step 3

Exam Tip

Recombinant DNA बनाने के लिए अलग DNA fragments को जोड़ना पड़ता है। Ligase यह joining करता है।

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Ligase enzyme का कौन सा statement false है?

Which statement about ligase enzyme is false?

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D. It cuts DNA at palindromic recognition sites

Step 1

Concept

Cutting DNA at palindromic recognition sites is the role of restriction enzymes. Ligase is a joining enzyme.

Step 2

Why this answer is correct

The correct answer is D. It cuts DNA at palindromic recognition sites. Cutting DNA at palindromic recognition sites is the role of restriction enzymes. Ligase is a joining enzyme.

Step 3

Exam Tip

Palindromic recognition sites पर DNA काटना restriction enzymes का काम है। Ligase joining enzyme है।

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Directional cloning ligase से जुड़े किस परिणाम को बेहतर कर सकती है?

Directional cloning can improve which result related to ligase?

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Correct Answer

A. insert को desired orientation में जुड़वानाJoining insert in desired orientation

Step 1

Concept

Directional cloning uses different ends to control insert orientation. Ligase then joins matching ends.

Step 2

Why this answer is correct

The correct answer is A. insert को desired orientation में जुड़वाना / Joining insert in desired orientation. Directional cloning uses different ends to control insert orientation. Ligase then joins matching ends.

Step 3

Exam Tip

Directional cloning अलग-अलग ends का उपयोग कर insert orientation को control करती है। Ligase फिर matching ends को जोड़ता है।

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Ligase और helicase का सही difference कौन सा है?

What is the correct difference between ligase and helicase?

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B. ligase joins DNA और helicase unwinds DNALigase joins DNA and helicase unwinds DNA

Step 1

Concept

Helicase unwinds the double helix. Ligase joins DNA fragments.

Step 2

Why this answer is correct

The correct answer is B. ligase joins DNA और helicase unwinds DNA / Ligase joins DNA and helicase unwinds DNA. Helicase unwinds the double helix. Ligase joins DNA fragments.

Step 3

Exam Tip

Helicase double helix खोलता है। Ligase DNA fragments को जोड़ता है।

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DNA polymerase और ligase में आसान फर्क क्या है?

What is the easy difference between DNA polymerase and ligase?

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Correct Answer

A. polymerase strand बढ़ाता है और ligase nick seal करता हैPolymerase extends strand and ligase seals nick

Step 1

Concept

Polymerase builds a strand by adding nucleotides. Ligase seals nicks between pre-existing DNA fragments.

Step 2

Why this answer is correct

The correct answer is A. polymerase strand बढ़ाता है और ligase nick seal करता है / Polymerase extends strand and ligase seals nick. Polymerase builds a strand by adding nucleotides. Ligase seals nicks between pre-existing DNA fragments.

Step 3

Exam Tip

Polymerase nucleotide जोड़कर strand बनाता है। Ligase pre-existing DNA fragments के बीच nick seal करता है।

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DNA replication में ligase की जरूरत मुख्य रूप से क्यों होती है?

Why is ligase mainly needed in DNA replication?

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Correct Answer

B. Okazaki fragments को जोड़ने के लिएTo join Okazaki fragments

Step 1

Concept

In replication Okazaki fragments form on the lagging strand. Ligase joins them to make a continuous strand.

Step 2

Why this answer is correct

The correct answer is B. Okazaki fragments को जोड़ने के लिए / To join Okazaki fragments. In replication Okazaki fragments form on the lagging strand. Ligase joins them to make a continuous strand.

Step 3

Exam Tip

Replication में lagging strand पर Okazaki fragments बनते हैं। Ligase उन्हें जोड़कर continuous strand बनाता है।

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Ligase enzyme का सबसे सरल सार क्या है?

What is the simplest summary of ligase enzyme?

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Correct Answer

D. यह डीएनए सिरों को जोड़ता हैIt joins DNA ends

Step 1

Concept

The core function of ligase is joining DNA ends. In Class 11 link it with recombinant DNA and replication.

Step 2

Why this answer is correct

The correct answer is D. यह डीएनए सिरों को जोड़ता है / It joins DNA ends. The core function of ligase is joining DNA ends. In Class 11 link it with recombinant DNA and replication.

Step 3

Exam Tip

Ligase का core function DNA ends जोड़ना है। Class 11 में इसे recombinant DNA और replication से जोड़ें।

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लाइगेज के बिना cloning में क्या मुख्य समस्या होगी?

Without ligase what will be the main problem in cloning?

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Correct Answer

D. cut vector और insert स्थायी रूप से नहीं जुड़ेंगेCut vector and insert will not join permanently

Step 1

Concept

Restriction enzyme can cut but ligase performs permanent joining. Therefore ligase is essential in cloning.

Step 2

Why this answer is correct

The correct answer is D. cut vector और insert स्थायी रूप से नहीं जुड़ेंगे / Cut vector and insert will not join permanently. Restriction enzyme can cut but ligase performs permanent joining. Therefore ligase is essential in cloning.

Step 3

Exam Tip

Restriction enzyme cutting कर सकता है लेकिन permanent joining ligase करता है। इसलिए ligase cloning में आवश्यक है।

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लाइगेशन प्रतिक्रिया में ऊर्जा स्रोत क्यों चाहिए?

Why is an energy source needed in a ligation reaction?

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Correct Answer

D. नया covalent बंध बनाने के लिएTo form a new covalent bond

Step 1

Concept

Ligase needs energy to form a new phosphodiester bond. Depending on enzyme type ATP or NAD plus can be used.

Step 2

Why this answer is correct

The correct answer is D. नया covalent बंध बनाने के लिए / To form a new covalent bond. Ligase needs energy to form a new phosphodiester bond. Depending on enzyme type ATP or NAD plus can be used.

Step 3

Exam Tip

लाइगेज को नया फॉस्फोडाइएस्टर बंध बनाने के लिए ऊर्जा चाहिए। enzyme के प्रकार के अनुसार एटीपी या एनएडी प्लस उपयोग हो सकता है।

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लाइगेज को अधिक गर्मी से क्यों बचाया जाता है?

Why is ligase protected from excess heat?

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Correct Answer

A. गर्मी से एंजाइम निष्क्रिय हो सकता हैHeat can inactivate the enzyme

Step 1

Concept

Ligase is a protein enzyme. Excess heat can denature it and reduce activity.

Step 2

Why this answer is correct

The correct answer is A. गर्मी से एंजाइम निष्क्रिय हो सकता है / Heat can inactivate the enzyme. Ligase is a protein enzyme. Excess heat can denature it and reduce activity.

Step 3

Exam Tip

लाइगेज प्रोटीन प्रकृति का एंजाइम है। अधिक गर्मी इसे denature करके activity घटा सकती है।

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लाइगेज को डीएनए गोंद क्यों कहा जाता है?

Why is ligase called DNA glue?

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Correct Answer

B. यह डीएनए टुकड़ों को जोड़ता हैIt joins DNA pieces

Step 1

Concept

Ligase forms permanent bonds between DNA pieces. Therefore the DNA glue comparison is correct.

Step 2

Why this answer is correct

The correct answer is B. यह डीएनए टुकड़ों को जोड़ता है / It joins DNA pieces. Ligase forms permanent bonds between DNA pieces. Therefore the DNA glue comparison is correct.

Step 3

Exam Tip

लाइगेज डीएनए टुकड़ों के बीच स्थायी बंध बनाता है। इसलिए डीएनए गोंद की तुलना सही है।

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