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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 451/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Restriction enzyme से बने fragments का size किस पर निर्भर करता है?

On what does the size of fragments made by restriction enzyme depend?

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B. cut sites की positions परOn positions of cut sites

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Cut sites जहाँ होंगे fragments की length वही तय करेगी। Restriction map इसी बात को दिखाता है। / Fragment length is determined by the positions of cut sites. A restriction map shows this.

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Question 452/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Restriction map बनाने में digestion data किसलिए उपयोगी होता है?

Why is digestion data useful in making a restriction map?

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C. cut site positions का अनुमान लगाने के लिएTo estimate cut site positions

Explanation

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Different digests के fragment sizes compare करके cut sites का अनुमान लगाया जाता है। यह mapping का basic idea है। / Cut site positions are estimated by comparing fragment sizes from different digests. This is the basic idea of mapping.

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Question 453/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Single digest restriction mapping में क्या जानकारी दे सकता है?

What information can a single digest give in restriction mapping?

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D. एक enzyme के cut sites से बने fragment sizesFragment sizes made by one enzyme cut sites

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Single digest एक enzyme का pattern देता है। Complete map के लिए double digests भी useful होते हैं। / A single digest gives the pattern for one enzyme. Double digests are also useful for a complete map.

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Question 454/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Double digest restriction map को कैसे बेहतर बनाता है?

How does double digest improve a restriction map?

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A. दो enzymes के sites की relative positions बताकरBy indicating relative positions of two enzyme sites

Explanation

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Double digest fragments sites के बीच की distances समझने में मदद करते हैं। इससे map अधिक clear होता है। / Double digest fragments help understand distances between sites. This makes the map clearer.

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Question 455/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Restriction mapping में complete digestion क्यों आवश्यक है?

Why is complete digestion necessary in restriction mapping?

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A. क्योंकि partial digestion false fragment pattern दे सकती हैBecause partial digestion can give a false fragment pattern

Explanation

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Incomplete cutting extra bands दे सकता है। Mapping में accurate band pattern बहुत जरूरी है। / Incomplete cutting can give extra bands. Accurate band pattern is very important in mapping.

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Question 456/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Partial digestion mapping में किस तरह भ्रम पैदा कर सकती है?

How can partial digestion create confusion in mapping?

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B. uncut और intermediate fragments दिखाकरBy showing uncut and intermediate fragments

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Partial digest में कुछ molecules fully cut नहीं होते। इससे actual map से अलग bands दिख सकते हैं। / In partial digest some molecules are not fully cut. This can show bands different from the actual map.

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Question 457/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Restriction fragment pattern में एक unexpected large band किसका संकेत हो सकता है?

What can an unexpected large band in restriction fragment pattern indicate?

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C. incomplete digestion

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Large band uncut या partially cut DNA का संकेत हो सकता है। Reaction time और enzyme activity check करें। / A large band can indicate uncut or partially cut DNA. Check reaction time and enzyme activity.

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Question 458/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Restriction fragment pattern में बहुत छोटे extra bands किससे जुड़ सकते हैं?

Very small extra bands in restriction fragment pattern can be linked with what?

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D. extra cut sites या star activityExtra cut sites or star activity

Explanation

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Extra small bands nonspecific cutting या additional sites दिखा सकते हैं। Controls से confirm करें। / Extra small bands can show nonspecific cutting or additional sites. Confirm with controls.

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Question 459/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Restriction enzyme reaction में substrate DNA का circular होना fragment count को कैसे प्रभावित करता है?

How does circular substrate DNA affect fragment count in restriction enzyme reaction?

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A. एक cut circular DNA को linear बना सकता हैOne cut can make circular DNA linear

Explanation

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Circular DNA एक site पर कटकर linear molecule बनता है। Linear DNA में वही एक cut दो fragments दे सकता है। / Circular DNA cut at one site becomes a linear molecule. The same one cut in linear DNA can give two fragments.

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Question 460/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Linear DNA में cut sites की संख्या और fragments की संख्या का सामान्य संबंध क्या है?

What is the general relation between number of cut sites and fragments in linear DNA?

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B. fragments सामान्यतः cut sites plus oneFragments generally equal cut sites plus one

Explanation

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Linear DNA में हर internal cut fragment count बढ़ाता है। इसलिए one cut से two fragments बनते हैं। / Each internal cut increases fragment count in linear DNA. Therefore one cut gives two fragments.

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