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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 471/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Restriction digest में underexposed gel image का क्या प्रभाव है?

What is the effect of an underexposed gel image in restriction digest?

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A. faint bands miss हो सकती हैंFaint bands can be missed

Explanation

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Underexposure weak DNA bands छिपा सकता है। Proper imaging result interpretation में जरूरी है। / Underexposure can hide weak DNA bands. Proper imaging is necessary for result interpretation.

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Question 472/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Restriction digestion में lane overloading से क्या समस्या होती है?

What problem occurs due to lane overloading in restriction digestion?

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A. bands diffuse या smear हो सकते हैंBands can become diffuse or smeared

Explanation

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बहुत ज्यादा DNA lane में साफ bands नहीं दे सकता। Proper loading amount रखें। / Too much DNA in a lane may not give clear bands. Keep proper loading amount.

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Question 473/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Restriction digest में sample wells damage होने से क्या हो सकता है?

What can happen if sample wells are damaged in restriction digest gel?

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A. sample leak या poor loading हो सकती हैSample leakage or poor loading can occur

Explanation

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Damaged wells sample को सही तरह hold नहीं करते। Gel casting और loading सावधानी से करें। / Damaged wells do not hold sample properly. Cast and load the gel carefully.

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Question 474/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Loading dye में dense component का उद्देश्य क्या है?

What is the purpose of the dense component in loading dye?

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A. sample को well में बैठाने में मददHelping sample settle in well

Explanation

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Dense component sample को well में नीचे बैठाता है। Dye front run progress दिखाता है। / The dense component helps sample settle in the well. Dye front shows run progress.

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Question 475/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Restriction digest sample में loading dye कब मिलाया जाता है?

When is loading dye mixed with restriction digest sample?

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A. gel loading से पहलेBefore gel loading

Explanation

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Loading dye gel पर sample load करने से पहले मिलाया जाता है। यह digestion enzyme नहीं है। / Loading dye is mixed before loading sample on gel. It is not a digestion enzyme.

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Question 476/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Gel run में DNA bands curved दिखें तो क्या कारण हो सकता है?

What can cause curved DNA bands in a gel run?

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Correct Answer

A. uneven heating या gel running issueUneven heating or gel running issue

Explanation

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High voltage या uneven gel condition band shape बिगाड़ सकती है। Proper voltage और buffer रखें। / High voltage or uneven gel condition can distort band shape. Keep proper voltage and buffer.

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Question 477/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Restriction digest gel में smiling bands क्या संकेत दे सकते हैं?

What can smiling bands in restriction digest gel indicate?

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A. uneven heating या high voltageUneven heating or high voltage

Explanation

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Smiling bands gel run condition problem दिखाते हैं। Voltage और buffer temperature control करें। / Smiling bands indicate gel running condition problem. Control voltage and buffer temperature.

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Question 478/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Restriction digestion में buffer reuse करने से क्या समस्या हो सकती है?

What problem can occur by reusing buffer in restriction digestion gel?

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A. poor conductivity और weak band resolutionPoor conductivity and weak band resolution

Explanation

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Old buffer ionic balance खो सकता है। Fresh running buffer clear separation में मदद करता है। / Old buffer can lose ionic balance. Fresh running buffer helps clear separation.

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Question 479/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Restriction digest में gel buffer और gel casting buffer same type क्यों होना चाहिए?

Why should gel buffer and gel casting buffer be the same type in restriction digest analysis?

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A. consistent ionic condition के लिएFor consistent ionic condition

Explanation

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Different buffers ionic mismatch पैदा कर सकते हैं। यह migration और resolution को affect कर सकता है। / Different buffers can create ionic mismatch. This can affect migration and resolution.

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Question 480/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 54

Restriction digest gel में DNA ladder गलत lane में load हो जाए तो क्या समस्या होगी?

What problem occurs if DNA ladder is loaded in the wrong lane?

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Correct Answer

A. lane interpretation confuse हो सकती हैLane interpretation can be confused

Explanation

Simple Explanation

Lane map सही न हो तो size comparison गलत हो सकता है। Loading order record करें। / If lane map is wrong size comparison can be incorrect. Record loading order.

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