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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 421/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction cut vector में दो अलग ends हों तो empty vector formation क्यों घट सकती है?

Why can empty vector formation decrease when restriction-cut vector has two different ends?

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A. ends आपस में easily compatible नहीं होतेEnds are not easily compatible with each other

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दो अलग ends vector self-closing को कम कर सकते हैं। Insert correct orientation में जोड़ने में मदद मिलती है। / Two different ends can reduce vector self-closing. They help join insert in correct orientation.

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Question 422/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Directional cloning का सबसे अच्छा outcome क्या है?

What is the best outcome of directional cloning?

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A. insert correct orientation में लगने की संभावना बढ़ती हैChance of insert joining in correct orientation increases

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दो different restriction ends orientation control में मदद करते हैं। इससे screening load कम हो सकता है। / Two different restriction ends help control orientation. This can reduce screening load.

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Question 423/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion में same enzyme से cut vector में orientation issue क्यों हो सकता है?

Why can orientation issue occur in a vector cut with the same enzyme?

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A. insert दोनों directions में ligate हो सकता हैInsert can ligate in both directions

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Same ends insert को either orientation में allow कर सकते हैं। Orientation screening जरूरी हो सकती है। / Same ends can allow insert in either orientation. Orientation screening may be needed.

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Question 424/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digest के बाद orientation screening के लिए कौन सा method useful हो सकता है?

Which method can be useful for orientation screening after restriction digest?

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A. diagnostic digest या colony PCRDiagnostic digest or colony PCR

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Diagnostic digest या PCR orientation-specific pattern दे सकता है। Final confirmation sequencing से करें। / Diagnostic digest or PCR can give orientation-specific pattern. Do final confirmation by sequencing.

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Question 425/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digestion में colony screening बाद में क्यों जरूरी हो सकती है?

Why can colony screening later be needed after restriction digestion?

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A. हर ligation product desired clone नहीं होताNot every ligation product is desired clone

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Cutting और ligation के बाद false clones मिल सकते हैं। Screening correct recombinant identify करती है। / After cutting and ligation false clones can appear. Screening identifies the correct recombinant.

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Question 426/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction cutting step में गलत enzyme use होने पर downstream effect क्या होगा?

What is the downstream effect of using a wrong enzyme in cutting step?

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A. ligation और transformation result खराब हो सकते हैंLigation and transformation results can fail

Explanation

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Wrong enzyme incompatible या damaged ends बना सकता है। इससे पूरा cloning workflow प्रभावित होता है। / Wrong enzyme can create incompatible or damaged ends. This affects the whole cloning workflow.

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Question 427/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Cutting step में incomplete digestion से ligation में क्या समस्या हो सकती है?

What problem can incomplete digestion in cutting step cause in ligation?

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A. uncut vector background colonies

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Uncut vector transform होकर background दे सकता है। Complete digestion और gel purification इसे घटाते हैं। / Uncut vector can transform and give background. Complete digestion and gel purification reduce it.

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Question 428/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Uncut vector background को कैसे घटाया जा सकता है?

How can uncut vector background be reduced?

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A. complete digestion और gel purification सेBy complete digestion and gel purification

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Complete digestion vector को linearizes करती है और gel purification uncut plasmid हटाता है। इससे background घटता है। / Complete digestion linearizes vector and gel purification removes uncut plasmid. This reduces background.

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Question 429/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digest में gel purification से uncut plasmid कैसे हट सकता है?

How can gel purification remove uncut plasmid in restriction digest?

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A. cut linear band को अलग excise करकेBy excising the cut linear band separately

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Uncut plasmid अलग mobility दिखा सकता है। Desired cut band को अलग purify किया जा सकता है। / Uncut plasmid can show different mobility. The desired cut band can be purified separately.

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Question 430/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 53

Restriction digest में small unwanted fragments ligation में क्या कर सकते हैं?

What can small unwanted fragments do in ligation after restriction digest?

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A. compete करके unwanted products बना सकते हैंThey can compete and form unwanted products

Explanation

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Unwanted fragments ligation reaction में interfere कर सकते हैं। Purification specific desired fragments रखती है। / Unwanted fragments can interfere in ligation reaction. Purification keeps specific desired fragments.

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