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A. residual ethanol हटाने के लिए/To remove residual ethanol
Step 1
Concept
Residual ethanol can inhibit ligation or digestion. But avoid over-drying too.
Step 2
Why this answer is correct
The correct answer is A. residual ethanol हटाने के लिए / To remove residual ethanol. Residual ethanol can inhibit ligation or digestion. But avoid over-drying too.
Step 3
Exam Tip
Residual ethanol ligation या digestion inhibit कर सकता है। लेकिन over-drying भी avoid करें।
B. accurate DNA quantification confuse हो सकती है/Accurate DNA quantification can be confused
Step 1
Concept
RNA contamination can affect DNA amount estimation. RNase treatment or cleanup can help.
Step 2
Why this answer is correct
The correct answer is B. accurate DNA quantification confuse हो सकती है / Accurate DNA quantification can be confused. RNA contamination can affect DNA amount estimation. RNase treatment or cleanup can help.
Step 3
Exam Tip
RNA contamination DNA amount estimate को प्रभावित कर सकती है। RNase treatment या cleanup helpful हो सकता है।
A. वे ligation में unwanted competition कर सकते हैं/They can cause unwanted competition in ligation
Step 1
Concept
Small DNA pieces with compatible ends can disturb ligation. Cleanup helps remove them.
Step 2
Why this answer is correct
The correct answer is A. वे ligation में unwanted competition कर सकते हैं / They can cause unwanted competition in ligation. Small DNA pieces with compatible ends can disturb ligation. Cleanup helps remove them.
Step 3
Exam Tip
छोटे DNA pieces compatible ends लेकर ligation reaction disturb कर सकते हैं। Cleanup इन्हें हटाने में मदद करता है।
A. cleanup या enzyme removal step use करें/Use cleanup or enzyme removal step
Step 1
Concept
A non-inactivated enzyme can remain active in next step. Cleanup helps stop unwanted cutting.
Step 2
Why this answer is correct
The correct answer is A. cleanup या enzyme removal step use करें / Use cleanup or enzyme removal step. A non-inactivated enzyme can remain active in next step. Cleanup helps stop unwanted cutting.
Step 3
Exam Tip
Non-inactivated enzyme next step में activity रख सकता है। Cleanup unwanted cutting रोकने में मदद करता है।
A. जब दूसरा buffer पहले से incompatible हो/When second buffer is incompatible with first
Step 1
Concept
Salts or enzyme from first reaction can affect the second reaction. Cleanup or buffer exchange can help.
Step 2
Why this answer is correct
The correct answer is A. जब दूसरा buffer पहले से incompatible हो / When second buffer is incompatible with first. Salts or enzyme from first reaction can affect the second reaction. Cleanup or buffer exchange can help.
Step 3
Exam Tip
पहले reaction के salts या enzyme दूसरे reaction को affect कर सकते हैं। Cleanup या buffer exchange helpful हो सकता है।
A. वे ligation reaction में unwanted competition दे सकते हैं/They can give unwanted competition in ligation reaction
Step 1
Concept
Small fragments can have compatible ends and confuse ligation. Cleanup removes them.
Step 2
Why this answer is correct
The correct answer is A. वे ligation reaction में unwanted competition दे सकते हैं / They can give unwanted competition in ligation reaction. Small fragments can have compatible ends and confuse ligation. Cleanup removes them.
Step 3
Exam Tip
Small fragments compatible ends रख सकते हैं और ligation में confuse कर सकते हैं। Cleanup इन्हें हटाता है।
A. जब buffer salts या enzymes next step affect करें/When buffer salts or enzymes affect next step
Step 1
Concept
Heat inactivation can stop enzyme but salts and proteins may remain. Cleanup can improve next reaction.
Step 2
Why this answer is correct
The correct answer is A. जब buffer salts या enzymes next step affect करें / When buffer salts or enzymes affect next step. Heat inactivation can stop enzyme but salts and proteins may remain. Cleanup can improve next reaction.
Step 3
Exam Tip
Heat inactivation enzyme रोक सकती है लेकिन salts और proteins रह सकते हैं। Cleanup next reaction को सुधार सकता है।
A. contaminants ligase activity रोक सकते हैं/Contaminants can inhibit ligase activity
Step 1
Concept
Salt ethanol phenol or enzymes can interfere with ligation. Clean DNA ends give better joining.
Step 2
Why this answer is correct
The correct answer is A. contaminants ligase activity रोक सकते हैं / Contaminants can inhibit ligase activity. Salt ethanol phenol or enzymes can interfere with ligation. Clean DNA ends give better joining.
Step 3
Exam Tip
Salt ethanol phenol या enzymes ligation में बाधा डाल सकते हैं। Clean DNA ends बेहतर joining देते हैं।
A. phosphatase हटाने के लिए जो ligation को affect कर सकती है/To remove phosphatase that can affect ligation
Step 1
Concept
Residual phosphatase can keep removing phosphate in ligation setup. Therefore cleanup is useful.
Step 2
Why this answer is correct
The correct answer is A. phosphatase हटाने के लिए जो ligation को affect कर सकती है / To remove phosphatase that can affect ligation. Residual phosphatase can keep removing phosphate in ligation setup. Therefore cleanup is useful.
Step 3
Exam Tip
Residual phosphatase ligation setup में phosphate removal जारी रख सकती है। इसलिए cleanup useful है।
A. cleanup में DNA loss हो सकता है/DNA loss can occur during cleanup
Step 1
Concept
Cleanup recovery is not complete. Actual DNA concentration is needed for ligation setup.
Step 2
Why this answer is correct
The correct answer is A. cleanup में DNA loss हो सकता है / DNA loss can occur during cleanup. Cleanup recovery is not complete. Actual DNA concentration is needed for ligation setup.
Step 3
Exam Tip
Cleanup recovery पूर्ण नहीं होती। Ligation setup के लिए actual DNA concentration जानना जरूरी है।
A. special cleanup steps से/By special cleanup steps
Step 1
Concept
Soil samples may contain humic substances. Cleanup steps reduce inhibitors.
Step 2
Why this answer is correct
The correct answer is A. special cleanup steps से / By special cleanup steps. Soil samples may contain humic substances. Cleanup steps reduce inhibitors.
Step 3
Exam Tip
Soil samples में humic substances हो सकते हैं। Cleanup steps inhibitors घटाते हैं।
