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124 questions tagged with dna-ligase.
Why is phosphate important at restriction-cut ends?
A. ligase द्वारा bond formation के लिएFor bond formation by ligase
DNA ligase phosphodiester bond बनाता है। इसके लिए सही chemical ends जरूरी हैं। / DNA ligase forms phosphodiester bonds. Correct chemical ends are needed for this.
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Which combination is most correct for making a recombinant vector?
A. Vector plus insert DNA plus DNA ligase
Recombinant vector बनाने के लिए insert को vector से जोड़ा जाता है। DNA ligase इस joining में मदद करता है। / To make a recombinant vector the insert is joined with the vector. DNA ligase helps in this joining.
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What is the role of DNA ligase in making a recombinant vector?
B. इंसर्ट और वेक्टर को जोड़नाJoining insert and vector
डीएनए लाइगेज वेक्टर और इंसर्ट के सिरों को जोड़ता है। restriction enzyme काटता है और ligase जोड़ता है। / DNA ligase joins the ends of vector and insert. Restriction enzyme cuts and ligase joins.
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Which situation increases the chance of recombinant vector formation?
A. Vector और insert compatible ends के साथ ligase से मिलेंVector and insert with compatible ends are mixed with ligase
Compatible ends और DNA ligase vector-insert joining में मदद करते हैं। इससे recombinant vector बन सकता है। / Compatible ends and DNA ligase help vector-insert joining. This can form a recombinant vector.
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Which enzyme is useful after vector and insert ends are ready to join?
A. DNA ligase
Restriction enzymes ends तैयार कर सकते हैं लेकिन joining DNA ligase करता है। यह recombinant vector बनाता है। / Restriction enzymes can prepare ends but DNA ligase performs joining. It makes the recombinant vector.
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Which combination is correct for making a recombinant vector?
A. Vector plus insert DNA plus ligase
Recombinant vector में vector और insert DNA जुड़े होते हैं। DNA ligase joining में मदद करता है। / A recombinant vector has vector and insert DNA joined together. DNA ligase helps in joining.
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Which enzyme performs joining when a foreign gene is added to a cloning vector?
A. डीएनए लाइगेजDNA ligase
DNA ligase foreign gene और vector ends को जोड़ता है। Restriction enzymes पहले compatible ends बना सकते हैं। / DNA ligase joins the foreign gene and vector ends. Restriction enzymes can create compatible ends first.
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Which enzyme helps join insert DNA into a vector?
A. डीएनए लाइगेजDNA ligase
DNA ligase insert और vector के सिरों को जोड़ता है। Restriction enzyme cut करता है और ligase join करता है। / DNA ligase joins the ends of insert and vector. Restriction enzyme cuts and ligase joins.
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Why is DNA ligase role considered less prominent on the leading strand?
A. leading strand continuous synthesis से बनता हैLeading strand is made by continuous synthesis
Leading strand continuous रूप से बनता है जबकि lagging strand पर Okazaki fragments बनते हैं। इसलिए ligase का fragment joining role lagging strand पर अधिक प्रमुख है। / The leading strand is made continuously while Okazaki fragments form on the lagging strand. Therefore ligase fragment joining role is more prominent on the lagging strand.
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Why are compatible ends important in ligase reaction?
A. वे सही pairing और joining में मदद करते हैंThey help proper pairing and joining
Compatible ends पास आकर ligase के लिए joining आसान करते हैं। Incompatible ends ligation efficiency घटा सकते हैं। / Compatible ends come close and make joining easier for ligase. Incompatible ends can reduce ligation efficiency.
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What is the best reason for calling ligase molecular glue?
A. यह DNA pieces को covalent bond से जोड़ता हैIt joins DNA pieces with covalent bond
Ligase DNA pieces को phosphodiester bond से जोड़ता है। इसलिए molecular glue analogy सही है। / Ligase joins DNA pieces with phosphodiester bonds. Therefore the molecular glue analogy is correct.
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Which option gives correct use of ligase in recombinant DNA?
A. different DNA fragments को जोड़नाJoining different DNA fragments
Recombinant DNA में अलग स्रोतों के DNA fragments जोड़े जाते हैं। Ligase इस joining को स्थायी बनाता है। / In recombinant DNA DNA fragments from different sources are joined. Ligase makes this joining permanent.
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Which option links ligase role with both replication and cloning?
A. Okazaki fragments और vector-insert joiningOkazaki fragments and vector-insert joining
Replication में ligase Okazaki fragments जोड़ता है और cloning में vector-insert जोड़ता है। यही दो मुख्य contexts हैं। / In replication ligase joins Okazaki fragments and in cloning it joins vector-insert. These are two main contexts.
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Which option correctly relates ligase and Okazaki fragments?
D. Ligase Okazaki fragments जोड़ता हैLigase joins Okazaki fragments
Ligase Okazaki fragments जोड़ता है और lagging strand को continuous बनाता है। यह replication का important point है। / Ligase joins Okazaki fragments and makes the lagging strand continuous. This is an important point in replication.
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By which process can sticky ends first come together?
B. complementary base pairing
Sticky ends में complementary overhangs होते हैं। वे पहले base pairing से पास आ सकते हैं और फिर ligase उन्हें जोड़ता है। / Sticky ends have complementary overhangs. They can first come together by base pairing and then ligase joins them.
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Which enzyme seals nicks between DNA fragments?
C. DNA ligase
DNA ligase DNA fragments के बीच nick seal करता है। इसे DNA replication और cloning दोनों में याद रखें। / DNA ligase seals nicks between DNA fragments. Remember it in both DNA replication and cloning.
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In a practical cut vector and insert are mixed. What should be added for stable joining?
A. डीएनए लाइगेजDNA ligase
डीएनए लाइगेज cut vector और insert के बीच phosphodiester bond बनाता है। इससे stable recombinant DNA बन सकता है। / DNA ligase forms phosphodiester bonds between cut vector and insert. This can make stable recombinant DNA.
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Which option gives a wrong relation between ligase and Okazaki fragments?
D. ligase creates Okazaki fragments by cutting DNA
Okazaki fragments DNA synthesis के दौरान बनते हैं। Ligase उन्हें काटता नहीं बल्कि जोड़ता है। / Okazaki fragments form during DNA synthesis. Ligase does not cut them but joins them.
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Which enzyme restores DNA backbone continuity?
A. DNA ligase
DNA ligase nicked backbone को seal कर continuity restore करता है। इसे repair और replication दोनों से जोड़ें। / DNA ligase seals the nicked backbone and restores continuity. Link it with both repair and replication.
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Which option is the most logical cause of ligation failure?
A. missing 5 prime phosphate
Missing 5 prime phosphate phosphodiester bond formation को रोक सकता है। बाकी options ligation में मदद करते हैं। / Missing 5 prime phosphate can prevent phosphodiester bond formation. The other options help ligation.
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In which reaction does ligase need DNA ends to be close?
A. ligation reaction
Ligase adjacent DNA ends के बीच bond बनाता है। इसलिए DNA ends का पास और aligned होना जरूरी है। / Ligase forms a bond between adjacent DNA ends. Therefore DNA ends must be close and aligned.
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Which enzyme plays a joining role opposite to molecular scissors?
C. DNA ligase
Molecular scissors restriction enzymes को कहा जाता है क्योंकि वे DNA काटते हैं। DNA ligase opposite joining role निभाता है। / Restriction enzymes are called molecular scissors because they cut DNA. DNA ligase performs the opposite joining role.
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Which enzyme can be considered molecular glue in cloning?
B. DNA ligase
DNA ligase cut DNA ends को जोड़ता है। इसलिए उसे cloning में molecular glue कहा जा सकता है। / DNA ligase joins cut DNA ends. Therefore it can be called molecular glue in cloning.
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Which option correctly links ligase and repair?
A. DNA nick को seal करनाSealing DNA nick
Ligase DNA repair में nick sealing कर सकता है। इससे DNA strand की continuity वापस आती है। / Ligase can perform nick sealing in DNA repair. This restores continuity of the DNA strand.
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In which condition can ligase reaction be less successful?
D. incompatible DNA ends होंIncompatible DNA ends are present
Incompatible DNA ends ठीक से pair नहीं कर पाएंगे। इससे ligation efficiency घट सकती है। / Incompatible DNA ends will not pair properly. This can reduce ligation efficiency.
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Temporary joining of sticky ends occurs by what?
B. base pairing
Sticky ends के complementary bases पहले base pairing कर सकते हैं। Ligase इस pairing को permanent bond में बदलता है। / Complementary bases of sticky ends can first base pair. Ligase converts this pairing into a permanent bond.
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What may happen if 5 prime phosphate is absent in ligation?
A. ligation efficiency घट सकती हैLigation efficiency may decrease
5 prime phosphate phosphodiester bond formation के लिए जरूरी है। इसकी कमी ligation को प्रभावित कर सकती है। / 5 prime phosphate is needed for phosphodiester bond formation. Its absence can affect ligation.
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Ligase mainly acts on which molecule?
C. डीएनएDNA
DNA ligase DNA के nicked या cut ends पर काम करता है। यह food-digesting enzyme नहीं है। / DNA ligase acts on nicked or cut ends of DNA. It is not a food-digesting enzyme.
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Which enzyme joins DNA ends but does not add new nucleotides?
C. DNA ligase
DNA ligase polymerase की तरह new nucleotides add नहीं करता। यह existing DNA ends को जोड़ता है। / DNA ligase does not add new nucleotides like polymerase. It joins existing DNA ends.
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DNA ligase is especially linked with which strand in replication?
A. पिछड़ा तंतुLagging strand
DNA ligase lagging strand के Okazaki fragments को जोड़ता है। यह replication MCQs में अक्सर पूछा जाता है। / DNA ligase joins Okazaki fragments of the lagging strand. This is often asked in replication MCQs.
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