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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 371/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digestion से पहले DNA cleanup कब helpful है?

When is DNA cleanup helpful before restriction digestion?

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A. जब sample में salts phenol या ethanol contamination होWhen sample has salts phenol or ethanol contamination

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Contaminants enzyme activity को रोक सकते हैं। Cleanup digestion efficiency सुधार सकता है। / Contaminants can block enzyme activity. Cleanup can improve digestion efficiency.

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Question 372/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digestion में sample concentration normalize क्यों करते हैं?

Why is sample concentration normalized in restriction digestion?

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A. different reactions में equal DNA amount रखने के लिएTo keep equal DNA amount in different reactions

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Equal DNA amount comparison को fair बनाता है। इससे gel band intensity interpretation बेहतर होती है। / Equal DNA amount makes comparison fair. This improves interpretation of gel band intensity.

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Question 373/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digest में pipetting accuracy क्यों critical है?

Why is pipetting accuracy critical in restriction digest?

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A. small volume में concentration बदल सकती हैConcentration can change in small volume

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Reaction में enzyme buffer और DNA सही मात्रा में होने चाहिए। Pipetting error digestion result बदल सकता है। / The reaction needs correct amounts of enzyme buffer and DNA. Pipetting error can change digestion result.

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Question 374/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digest में reaction volume बड़ा करने का एक benefit क्या हो सकता है?

What is one benefit of increasing reaction volume in restriction digest?

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A. pipetting error का relative effect घट सकता हैRelative effect of pipetting error can decrease

Explanation

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बहुत छोटे volumes में errors अधिक प्रभाव डालते हैं। उचित volume accuracy में मदद करता है। / Errors have larger effect in very small volumes. Suitable volume helps accuracy.

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Question 375/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digest incubation में tube cap tight क्यों होना चाहिए?

Why should tube cap be tight during restriction digest incubation?

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A. evaporation रोकने के लिएTo prevent evaporation

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Evaporation reaction concentration बदल सकती है। Tight cap reaction condition स्थिर रखती है। / Evaporation can change reaction concentration. A tight cap keeps reaction condition stable.

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Question 376/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digest में spin-down का practical purpose क्या है?

What is the practical purpose of spin-down in restriction digest?

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A. drops को tube bottom में लानाBringing drops to tube bottom

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Spin-down tube wall पर लगी droplets को नीचे लाता है। इससे reaction complete volume में होती है। / Spin-down brings droplets on tube wall to the bottom. Thus reaction occurs in complete volume.

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Question 377/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digest mix को briefly centrifuge करने से क्या फायदा है?

What is the benefit of briefly centrifuging restriction digest mix?

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A. components नीचे collect होकर proper reaction देते हैंComponents collect at bottom for proper reaction

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Brief spin reaction droplets को bottom में लाता है। इससे mixing और incubation consistent होती है। / A brief spin brings reaction droplets to bottom. This makes mixing and incubation consistent.

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Question 378/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digest में harsh shaking क्यों avoid करनी चाहिए?

Why should harsh shaking be avoided in restriction digest?

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A. long DNA shearing और enzyme damage risk से बचने के लिएTo avoid long DNA shearing and enzyme damage risk

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Gentle mixing DNA integrity और enzyme activity बचाता है। Harsh handling unnecessary है। / Gentle mixing protects DNA integrity and enzyme activity. Harsh handling is unnecessary.

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Question 379/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digestion में final reaction को gently tap करने का उद्देश्य क्या है?

What is the purpose of gently tapping the final reaction in restriction digestion?

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A. components को मिलाना without damageMixing components without damage

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Gentle tapping reaction mix को uniform करता है। इससे components ठीक से contact में आते हैं। / Gentle tapping makes reaction mix uniform. This brings components into proper contact.

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Question 380/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digest में reaction setup on ice कब useful है?

When is reaction setup on ice useful in restriction digest?

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A. enzyme stability और premature activity control के लिएFor enzyme stability and controlling premature activity

Explanation

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Ice enzyme को stable रखता है। Reaction desired incubation temperature पर शुरू की जाती है। / Ice keeps enzyme stable. The reaction is started at desired incubation temperature.

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