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reaction setup MCQ Questions for Class 11

reaction setup se related questions ko ek jagah revise karein. Har question me bilingual content, answer feedback aur explanation available hai.

Practice Questions

8 questions tagged with reaction setup.

Restriction digest में spin-down का practical purpose क्या है?

What is the practical purpose of spin-down in restriction digest?

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Correct Answer

A. drops को tube bottom में लानाBringing drops to tube bottom

Step 1

Concept

Spin-down brings droplets on tube wall to the bottom. Thus reaction occurs in complete volume.

Step 2

Why this answer is correct

The correct answer is A. drops को tube bottom में लाना / Bringing drops to tube bottom. Spin-down brings droplets on tube wall to the bottom. Thus reaction occurs in complete volume.

Step 3

Exam Tip

Spin-down tube wall पर लगी droplets को नीचे लाता है। इससे reaction complete volume में होती है।

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Restriction digestion के लिए DNA quantification क्यों किया जाता है?

Why is DNA quantification done for restriction digestion?

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Correct Answer

A. enzyme और DNA amount सही रखने के लिएTo keep enzyme and DNA amount correct

Step 1

Concept

Knowing DNA amount helps set enzyme units and reaction correctly. This makes digestion reliable.

Step 2

Why this answer is correct

The correct answer is A. enzyme और DNA amount सही रखने के लिए / To keep enzyme and DNA amount correct. Knowing DNA amount helps set enzyme units and reaction correctly. This makes digestion reliable.

Step 3

Exam Tip

DNA amount जानकर enzyme units और reaction setup सही किया जाता है। इससे digestion reliable होती है।

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Restriction digest setup में bubbles क्यों avoid करते हैं?

Why are bubbles avoided in restriction digest setup?

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Correct Answer

A. accurate mixing और pipetting maintain करने के लिएTo maintain accurate mixing and pipetting

Step 1

Concept

Bubbles can cause inaccurate volume and mixing in small reactions. Gentle pipetting is better.

Step 2

Why this answer is correct

The correct answer is A. accurate mixing और pipetting maintain करने के लिए / To maintain accurate mixing and pipetting. Bubbles can cause inaccurate volume and mixing in small reactions. Gentle pipetting is better.

Step 3

Exam Tip

Bubbles small reaction volumes में inaccurate volume और mixing दे सकते हैं। Gentle pipetting बेहतर है।

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Reaction mix में enzyme last में add करने का practical कारण क्या है?

What is the practical reason for adding enzyme last in reaction mix?

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Correct Answer

A. enzyme exposure और timing control करनाTo control enzyme exposure and timing

Step 1

Concept

Other components are mixed at correct concentration first. Adding enzyme last gives better reaction timing control.

Step 2

Why this answer is correct

The correct answer is A. enzyme exposure और timing control करना / To control enzyme exposure and timing. Other components are mixed at correct concentration first. Adding enzyme last gives better reaction timing control.

Step 3

Exam Tip

बाकी components पहले सही concentration में मिल जाते हैं। Enzyme last में डालने से reaction timing better control होती है।

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Reaction setup में labels क्यों जरूरी हैं?

Why are labels important in reaction setup?

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Correct Answer

C. sample mix-up रोकने के लिएTo prevent sample mix-up

Step 1

Concept

Clear labels prevent wrong enzyme or wrong sample mix-up. This is necessary for reliable results.

Step 2

Why this answer is correct

The correct answer is C. sample mix-up रोकने के लिए / To prevent sample mix-up. Clear labels prevent wrong enzyme or wrong sample mix-up. This is necessary for reliable results.

Step 3

Exam Tip

Clear labels wrong enzyme या wrong sample mix-up से बचाते हैं। यह reliable result के लिए जरूरी है।

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Reaction tube को gently mix क्यों किया जाता है?

Why is the reaction tube mixed gently?

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Correct Answer

A. DNA और enzyme evenly mix करने के लिए without shearingTo mix DNA and enzyme evenly without shearing

Step 1

Concept

Gentle mixing combines reaction components and avoids DNA damage. Avoid harsh vortexing.

Step 2

Why this answer is correct

The correct answer is A. DNA और enzyme evenly mix करने के लिए without shearing / To mix DNA and enzyme evenly without shearing. Gentle mixing combines reaction components and avoids DNA damage. Avoid harsh vortexing.

Step 3

Exam Tip

Gentle mixing reaction components मिलाता है और DNA damage से बचाता है। Harsh vortexing avoid करें।

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Restriction digestion setup में enzyme को अंत में क्यों add किया जाता है?

Why is enzyme added last in restriction digestion setup?

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Correct Answer

B. reaction conditions ready होने के बाद enzyme exposure control करने के लिएTo control enzyme exposure after reaction conditions are ready

Step 1

Concept

Adding enzyme last ensures reaction conditions are correct first. It helps control activity and timing.

Step 2

Why this answer is correct

The correct answer is B. reaction conditions ready होने के बाद enzyme exposure control करने के लिए / To control enzyme exposure after reaction conditions are ready. Adding enzyme last ensures reaction conditions are correct first. It helps control activity and timing.

Step 3

Exam Tip

Enzyme last में add करने से reaction conditions पहले correct हो जाती हैं। यह activity और timing control में मदद करता है।

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Restriction enzyme unit का अर्थ क्या है?

What does a restriction enzyme unit mean?

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Correct Answer

C. defined condition में DNA काटने की activity amountAmount of activity to cut DNA under defined condition

Step 1

Concept

Enzyme units describe activity. Enzyme amount is chosen according to DNA amount in reaction setup.

Step 2

Why this answer is correct

The correct answer is C. defined condition में DNA काटने की activity amount / Amount of activity to cut DNA under defined condition. Enzyme units describe activity. Enzyme amount is chosen according to DNA amount in reaction setup.

Step 3

Exam Tip

Enzyme units activity बताते हैं। Reaction setup में DNA amount के अनुसार enzyme मात्रा चुनी जाती है।

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