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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 351/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Single enzyme diagnostic digest की limitation क्या है?

What is a limitation of single enzyme diagnostic digest?

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A. यह orientation की जानकारी हमेशा नहीं देताIt does not always give orientation information

Explanation

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Single enzyme कभी केवल plasmid size या linearization दिखाता है। Orientation के लिए additional sites चाहिए। / A single enzyme may only show plasmid size or linearization. Additional sites are needed for orientation.

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Question 352/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Two enzyme diagnostic digest orientation में क्यों मदद कर सकता है?

Why can a two-enzyme diagnostic digest help in orientation?

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A. different orientation में different fragment sizes मिल सकते हैंDifferent orientations can give different fragment sizes

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दो sites की relative position orientation के साथ बदलती है। इसलिए band pattern informative हो सकता है। / Relative positions of two sites change with orientation. Therefore band pattern can be informative.

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Question 353/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digest में uncut plasmid lane क्यों रखना उपयोगी है?

Why is keeping an uncut plasmid lane useful in restriction digest?

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A. plasmid conformations और cut result compare करने के लिएTo compare plasmid conformations and cut result

Explanation

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Uncut plasmid supercoiled और open circular forms दिखा सकता है। Cut sample से comparison digestion समझाता है। / Uncut plasmid can show supercoiled and open circular forms. Comparison with cut sample explains digestion.

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Question 354/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Supercoiled plasmid का apparent size gel पर क्यों misleading हो सकता है?

Why can apparent size of supercoiled plasmid be misleading on gel?

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A. compact conformation faster migrate कर सकती हैCompact conformation can migrate faster

Explanation

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Supercoiled form linear DNA से अलग mobility रखता है। Size estimate के लिए linearized plasmid बेहतर है। / Supercoiled form has different mobility than linear DNA. Linearized plasmid is better for size estimate.

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Question 355/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Linearized plasmid size estimation में क्यों बेहतर हो सकता है?

Why can linearized plasmid be better for size estimation?

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A. linear DNA ladder से अधिक comparable होता हैIt is more comparable with linear DNA ladder

Explanation

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DNA ladder linear fragments से बनी होती है। Linearized plasmid का comparison अधिक सही होता है। / DNA ladder is made of linear fragments. Comparison of linearized plasmid is more accurate.

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Question 356/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Open circular plasmid band का क्या कारण हो सकता है?

What can cause an open circular plasmid band?

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A. single strand nick या relaxed plasmid formSingle strand nick or relaxed plasmid form

Explanation

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Nick होने पर supercoiled plasmid open circular बन सकता है। यह uncut lane में दिख सकता है। / A nick can make supercoiled plasmid open circular. This can appear in uncut lane.

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Question 357/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digest में nicked plasmid क्यों confusion दे सकता है?

Why can a nicked plasmid create confusion in restriction digest?

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A. वह अलग mobility दिखाकर extra band जैसा लग सकता हैIt can show different mobility and look like an extra band

Explanation

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Plasmid conformation अलग bands दे सकती है। इसलिए uncut control interpretation में मदद करता है। / Plasmid conformation can give different bands. Therefore uncut control helps interpretation.

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Question 358/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Complete digestion की confirmation कैसे हो सकती है?

How can complete digestion be confirmed?

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A. expected bands और uncut band की absence देखकरBy seeing expected bands and absence of uncut band

Explanation

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Complete digest में expected cut fragments दिखते हैं। Uncut band नहीं रहना चाहिए। / In complete digest expected cut fragments appear. Uncut band should not remain.

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Question 359/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Partial digestion gel पर कैसे दिख सकती है?

How can partial digestion appear on gel?

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A. expected bands के साथ uncut या larger intermediate bandsUncut or larger intermediate bands with expected bands

Explanation

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Partial digestion में कुछ molecules fully cut नहीं होते। इसलिए intermediate bands आ सकते हैं। / In partial digestion some molecules are not fully cut. Therefore intermediate bands can appear.

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Question 360/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Partial digest को सुधारने के लिए क्या किया जा सकता है?

What can be done to improve a partial digest?

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A. enzyme amount time और buffer condition optimize करनाOptimize enzyme amount time and buffer condition

Explanation

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Partial digestion अक्सर insufficient enzyme time या wrong condition से होती है। Recommended conditions follow करें। / Partial digestion often occurs due to insufficient enzyme time or wrong condition. Follow recommended conditions.

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