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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

Cutting of DNA Practice Questions

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Question 341/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 51

Level 51 का best revision point क्या है?

What is the best revision point of Level 51?

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A. enzyme choice vector map ends gel controls और safety याद रखेंRemember enzyme choice vector map ends gel controls and safety

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Cutting plan में map और enzyme conditions दोनों जरूरी हैं। Result को controls और gel से verify करें। / Both map and enzyme conditions are necessary in cutting plan. Verify result with controls and gel.

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Question 342/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 51

Cutting of DNA level 51 का final summary क्या है?

What is the final summary of Cutting of DNA level 51?

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A. सही restriction enzyme से vector और insert को planned compatible ends में काटा जाता हैCorrect restriction enzyme cuts vector and insert into planned compatible ends

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यह step cloning की दिशा और success तय करता है। Accurate maps clean DNA और controls जरूरी हैं। / This step decides cloning direction and success. Accurate maps clean DNA and controls are needed.

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Question 343/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digestion में quality control का मुख्य लक्ष्य क्या है?

What is the main goal of quality control in restriction digestion?

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A. cutting result reliable है या नहीं देखनाTo check whether cutting result is reliable

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Quality control से digestion success और errors समझ आते हैं। Controls और expected bands इसमें मदद करते हैं। / Quality control helps understand digestion success and errors. Controls and expected bands help in this.

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Question 344/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digestion में expected pattern कहाँ से मिलता है?

From where is the expected pattern obtained in restriction digestion?

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A. restriction map या sequence analysis सेFrom restriction map or sequence analysis

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Restriction map cut sites और fragment sizes बताता है। Expected pattern इसी से बनता है। / A restriction map shows cut sites and fragment sizes. The expected pattern is made from it.

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Question 345/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Observed pattern किससे मिलता है?

From what is the observed pattern obtained?

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A. gel electrophoresis result सेFrom gel electrophoresis result

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Observed bands gel पर दिखाई देते हैं। इन्हें expected pattern से compare किया जाता है। / Observed bands are seen on the gel. They are compared with expected pattern.

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Question 346/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Expected और observed patterns match हों तो क्या संकेत है?

What does it indicate if expected and observed patterns match?

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A. digestion या clone सही हो सकता हैDigestion or clone may be correct

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Matching bands correct digestion का support देते हैं। Final sequence confirmation के लिए sequencing बेहतर हो सकती है। / Matching bands support correct digestion. Sequencing can be better for final sequence confirmation.

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Question 347/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Restriction digest से exact DNA sequence क्यों नहीं पता चलती?

Why does restriction digest not reveal exact DNA sequence?

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A. यह केवल fragment size pattern देता हैIt gives only fragment size pattern

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Restriction digest size information देता है। Exact bases जानने के लिए sequencing चाहिए। / Restriction digest gives size information. Sequencing is needed to know exact bases.

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Question 348/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Sequencing restriction digest के बाद क्यों useful हो सकती है?

Why can sequencing be useful after restriction digest?

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A. exact insert sequence confirm करने के लिएTo confirm exact insert sequence

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Digest correct size दिखा सकता है लेकिन mutation नहीं बता सकता। Sequencing final confirmation देती है। / Digest can show correct size but cannot reveal mutation. Sequencing gives final confirmation.

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Question 349/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Diagnostic digest में false positive कब मिल सकता है?

When can a false positive occur in diagnostic digest?

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A. जब similar band pattern wrong construct से भी मिलेWhen a similar band pattern also comes from a wrong construct

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कभी wrong construct भी similar size bands दे सकता है। इसलिए informative enzymes और sequencing useful हैं। / Sometimes a wrong construct can also give similar size bands. Therefore informative enzymes and sequencing are useful.

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Question 350/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 52

Informative enzyme selection का मतलब क्या है?

What does informative enzyme selection mean?

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A. ऐसे enzymes चुनना जो correct और wrong construct में फर्क दिखाएँChoosing enzymes that distinguish correct and wrong construct

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Informative digestion अलग expected outcomes देता है। इससे clone verification मजबूत होती है। / Informative digestion gives different expected outcomes. This makes clone verification stronger.

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