Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 361-370 of 592 questions.
What can appear in gel pattern due to star activity?
A. unexpected extra fragments
Star activity non-specific cutting दे सकती है। Gel पर extra unexpected bands दिख सकते हैं। / Star activity can give non-specific cutting. Extra unexpected bands can appear on gel.
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Which step helps reduce star activity?
A. recommended buffer और limited enzyme volumeRecommended buffer and limited enzyme volume
Correct buffer enzyme specificity बनाए रखता है। Excess enzyme और glycerol avoid करें। / Correct buffer maintains enzyme specificity. Avoid excess enzyme and glycerol.
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How can methylation block be identified in restriction digestion?
A. methylation-sensitive और insensitive enzymes compare करकेBy comparing methylation-sensitive and insensitive enzymes
अगर methylation-sensitive enzyme cut न करे तो methylation cause हो सकता है। Alternative enzyme या host source मदद कर सकता है। / If a methylation-sensitive enzyme does not cut methylation can be a cause. Alternative enzyme or host source can help.
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Dam methylation is related to which base?
A. adenine methylation in certain bacterial sites
Dam methylation bacterial DNA में adenine modification से जुड़ी है। यह कुछ enzyme cutting को प्रभावित कर सकती है। / Dam methylation is linked with adenine modification in bacterial DNA. It can affect cutting by some enzymes.
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Dcm methylation is related to what?
A. cytosine methylation in certain bacterial sequences
Dcm methylation bacterial DNA में cytosine modification से जुड़ी है। कुछ restriction enzymes इससे प्रभावित हो सकते हैं। / Dcm methylation is linked with cytosine modification in bacterial DNA. Some restriction enzymes can be affected by it.
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From where can DNA be prepared to avoid methylation problem?
A. suitable methylation-deficient host strain सेFrom a suitable methylation-deficient host strain
Methylation-deficient host कुछ sensitive enzymes के लिए DNA suitable बना सकता है। Enzyme requirement पहले check करें। / A methylation-deficient host can make DNA suitable for some sensitive enzymes. Check enzyme requirements first.
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What happens if EDTA is high in restriction enzyme reaction?
A. magnesium chelation से enzyme activity घट सकती हैEnzyme activity can decrease due to magnesium chelation
EDTA magnesium ions bind करता है। कई restriction enzymes magnesium dependent होते हैं। / EDTA binds magnesium ions. Many restriction enzymes are magnesium dependent.
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When can DNA stored in TE buffer cause an issue in restriction digest?
A. अगर EDTA concentration reaction में बहुत अधिक होIf EDTA concentration is too high in reaction
TE buffer का EDTA magnesium chelate कर सकता है। Reaction buffer के अनुसार dilution या cleanup करें। / EDTA in TE buffer can chelate magnesium. Dilute or cleanup according to reaction buffer.
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What can be the effect of phenol carryover in restriction digest?
A. enzyme inhibition
Phenol enzyme proteins को affect कर सकता है। Clean DNA digestion के लिए जरूरी है। / Phenol can affect enzyme proteins. Clean DNA is necessary for digestion.
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How can ethanol carryover be suspected in restriction digest?
A. poor enzyme reaction और wet DNA pellet history सेFrom poor enzyme reaction and wet DNA pellet history
Residual ethanol enzyme reaction inhibit कर सकता है। Pellet proper dry होना चाहिए। / Residual ethanol can inhibit enzyme reaction. Pellet should be properly dried.
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