Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 321-330 of 592 questions.
What does dye front indicate in gel electrophoresis?
A. run progress का visual markerA visual marker of run progress
Loading dye का front gel run progress दिखाता है। यह बताता है कि sample कितना आगे चला। / The loading dye front shows gel run progress. It tells how far the sample has moved.
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What problem can occur if electrophoresis buffer is exhausted?
A. poor migration और unclear bandsPoor migration and unclear bands
Gel buffer ions current carry करने में मदद करते हैं। Poor buffer band resolution को खराब कर सकता है। / Gel buffer ions help carry current. Poor buffer can reduce band resolution.
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What happens if wrong polarity is applied in a restriction digest gel?
A. DNA गलत दिशा में जा सकता हैDNA can move in the wrong direction
DNA negative charged होता है और positive electrode की ओर चलता है। Wrong polarity sample को wells से बाहर ले जा सकती है। / DNA is negatively charged and moves toward positive electrode. Wrong polarity can move sample out of wells.
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Why are saturated bands a problem in gel image after restriction digestion?
A. accurate comparison कठिन हो जाता हैAccurate comparison becomes difficult
Overexposed image में band intensity सही compare नहीं होती। Documentation में proper exposure रखें। / In an overexposed image band intensity cannot be compared well. Use proper exposure in documentation.
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Why can an insert band be faint and vector band bright in a restriction digest?
A. insert size या amount कम हो सकता हैInsert size or amount may be lower
छोटे या कम quantity fragments कम bright दिख सकते हैं। Band intensity DNA mass पर निर्भर करती है। / Small or low quantity fragments can look less bright. Band intensity depends on DNA mass.
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Gel band intensity is mainly related to what?
A. उस band में DNA mass सेDNA mass in that band
अधिक DNA mass वाला band अधिक bright दिख सकता है। Size और concentration दोनों intensity को प्रभावित करते हैं। / A band with more DNA mass can appear brighter. Both size and concentration affect intensity.
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Why is low-salt clean DNA considered good in restriction digestion?
A. enzyme activity बेहतर रहने के लिएTo keep enzyme activity better
Excess salt restriction enzyme activity को बदल सकता है। Clean DNA digestion reliability बढ़ाता है। / Excess salt can alter restriction enzyme activity. Clean DNA increases digestion reliability.
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Why is final salt condition important in a restriction digest reaction?
A. enzyme binding और specificity प्रभावित होती हैEnzyme binding and specificity are affected
Restriction enzymes recommended salt conditions में best काम करते हैं। गलत salt partial digestion या star activity दे सकता है। / Restriction enzymes work best in recommended salt conditions. Wrong salt can give partial digestion or star activity.
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Why should a restriction enzyme not be vigorously vortexed?
A. enzyme protein damage या activity loss का risk होता हैThere is risk of enzyme protein damage or activity loss
Enzymes delicate proteins होते हैं। Gentle mixing enzyme activity को बचाता है। / Enzymes are delicate proteins. Gentle mixing preserves enzyme activity.
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Why is it good to close a restriction enzyme tube quickly?
A. temperature exposure और contamination कम करने के लिएTo reduce temperature exposure and contamination
Enzyme लंबे समय तक open या warm रहे तो activity घट सकती है। Quick handling good practice है। / If enzyme remains open or warm for long its activity can decrease. Quick handling is good practice.
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