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A. self-ligation कम करने के लिए/To reduce self-ligation
Step 1
Concept
Removing phosphate can reduce self-joining of vector ends. This increases chance of insert-containing clones.
Step 2
Why this answer is correct
The correct answer is A. self-ligation कम करने के लिए / To reduce self-ligation. Removing phosphate can reduce self-joining of vector ends. This increases chance of insert-containing clones.
Step 3
Exam Tip
Phosphate हटाने से vector ends का self-joining कम हो सकता है। इससे insert-containing clones की संभावना बढ़ती है।
A. vector ends का insert बिना आपस में जुड़ना/Vector ends joining each other without insert
Step 1
Concept
Self-ligation can give empty vector colonies. Dephosphorylation or directional cloning reduces it.
Step 2
Why this answer is correct
The correct answer is A. vector ends का insert बिना आपस में जुड़ना / Vector ends joining each other without insert. Self-ligation can give empty vector colonies. Dephosphorylation or directional cloning reduces it.
Step 3
Exam Tip
Self-ligation empty vector colonies दे सकती है। Dephosphorylation या directional cloning इसे घटाती है।
A. दो अलग incompatible vector ends बनाकर/By creating two different incompatible vector ends
Step 1
Concept
Different ends can prevent the vector from closing in the same orientation. Insert can join in correct orientation.
Step 2
Why this answer is correct
The correct answer is A. दो अलग incompatible vector ends बनाकर / By creating two different incompatible vector ends. Different ends can prevent the vector from closing in the same orientation. Insert can join in correct orientation.
Step 3
Exam Tip
Different ends vector को same orientation में close होने से रोक सकते हैं। Insert correct orientation में लग सकता है।
A. single enzyme sticky ends compatible हों/Single enzyme sticky ends are compatible
Step 1
Concept
Same compatible ends can let vector ligate to itself. Dephosphorylation or directional cloning can reduce this.
Step 2
Why this answer is correct
The correct answer is A. single enzyme sticky ends compatible हों / Single enzyme sticky ends are compatible. Same compatible ends can let vector ligate to itself. Dephosphorylation or directional cloning can reduce this.
Step 3
Exam Tip
Same compatible ends vector को खुद से ligate कर सकते हैं। Dephosphorylation या directional cloning इसे घटा सकती है।
A. vector self-ligation घटाना/Reducing vector self-ligation
Step 1
Concept
Removing phosphate reduces the chance of vector ends joining themselves. This can increase recombinant clone proportion.
Step 2
Why this answer is correct
The correct answer is A. vector self-ligation घटाना / Reducing vector self-ligation. Removing phosphate reduces the chance of vector ends joining themselves. This can increase recombinant clone proportion.
Step 3
Exam Tip
Phosphate हटाने से vector ends अपने आप जुड़ने की संभावना कम होती है। इससे recombinant clone proportion बढ़ सकता है।
Alkaline phosphatase removes phosphate from vector ends and can reduce self-ligation. This helps cloning efficiency.
Step 2
Why this answer is correct
The correct answer is B. alkaline phosphatase treatment. Alkaline phosphatase removes phosphate from vector ends and can reduce self-ligation. This helps cloning efficiency.
Step 3
Exam Tip
Alkaline phosphatase vector ends से phosphate हटाकर self-ligation घटा सकता है। यह cloning efficiency में मदद करता है।
A. Self-ligation कम करने के लिए/To reduce self-ligation
Step 1
Concept
Dephosphorylation can reduce vector self-ligation. This can increase the chance of recombinant colonies.
Step 2
Why this answer is correct
The correct answer is A. Self-ligation कम करने के लिए / To reduce self-ligation. Dephosphorylation can reduce vector self-ligation. This can increase the chance of recombinant colonies.
Step 3
Exam Tip
Dephosphorylation vector self-ligation को घटा सकती है। इससे recombinant colonies की संभावना बढ़ सकती है।
An empty vector can be selected due to the marker but lacks insert. It is identified by screening.
Step 2
Why this answer is correct
The correct answer is A. Empty vector self-ligation. An empty vector can be selected due to the marker but lacks insert. It is identified by screening.
Step 3
Exam Tip
Empty vector marker के कारण selected हो सकता है लेकिन insert नहीं रखता। इसे screening से पहचाना जाता है।
A. Vector insert के बिना बंद होकर marker रख सकता है/Vector can close without insert and still carry marker
Step 1
Concept
A self-ligated vector can keep the antibiotic marker but lacks desired insert. Therefore screening is needed.
Step 2
Why this answer is correct
The correct answer is A. Vector insert के बिना बंद होकर marker रख सकता है / Vector can close without insert and still carry marker. A self-ligated vector can keep the antibiotic marker but lacks desired insert. Therefore screening is needed.
Step 3
Exam Tip
Self-ligated vector antibiotic marker रख सकता है पर desired insert नहीं होता। इसलिए screening जरूरी है।
A. दो अलग ends से self-joining कठिन हो सकती है/Two different ends can make self-joining difficult
Step 1
Concept
Two different restriction ends can reduce vector self-ligation. This can improve desired insert cloning.
Step 2
Why this answer is correct
The correct answer is A. दो अलग ends से self-joining कठिन हो सकती है / Two different ends can make self-joining difficult. Two different restriction ends can reduce vector self-ligation. This can improve desired insert cloning.
Step 3
Exam Tip
दो अलग restriction ends vector के self-ligation को घटा सकते हैं। इससे desired insert cloning बेहतर हो सकती है।
Removing phosphate from vector ends can reduce self-ligation. This helps reduce background colonies.
Step 2
Why this answer is correct
The correct answer is A. Vector dephosphorylation. Removing phosphate from vector ends can reduce self-ligation. This helps reduce background colonies.
Step 3
Exam Tip
Vector ends से phosphate हटाने पर self-ligation घट सकती है। यह background colonies कम करने में मदद करता है।
A. Vector insert के बिना बंद हो जाता है/Vector closes without insert
Step 1
Concept
In self-ligation the vector joins its own ends. This does not form the desired recombinant clone.
Step 2
Why this answer is correct
The correct answer is A. Vector insert के बिना बंद हो जाता है / Vector closes without insert. In self-ligation the vector joins its own ends. This does not form the desired recombinant clone.
Step 3
Exam Tip
Self-ligation में vector अपने ही सिरों से जुड़ जाता है। इससे desired recombinant clone नहीं बनता।
Alkaline phosphatase can remove phosphate from vector ends. This can reduce vector self-ligation.
Step 2
Why this answer is correct
The correct answer is A. alkaline phosphatase treatment. Alkaline phosphatase can remove phosphate from vector ends. This can reduce vector self-ligation.
Step 3
Exam Tip
Alkaline phosphatase vector ends से phosphate हटा सकता है। इससे vector self-ligation कम हो सकती है।
A. self-ligation घट सकती है/Self-ligation can decrease
Step 1
Concept
Removing 5 prime phosphate from vector can reduce joining of its own ends. This helps control self-ligation.
Step 2
Why this answer is correct
The correct answer is A. self-ligation घट सकती है / Self-ligation can decrease. Removing 5 prime phosphate from vector can reduce joining of its own ends. This helps control self-ligation.
Step 3
Exam Tip
Vector से 5 prime phosphate हटाने पर उसके अपने ends की joining घट सकती है। यह self-ligation control में मदद करता है।
A. 5 prime phosphate हटने से vector अपने ends आसानी से नहीं जोड़ पाएगा/Removal of 5 prime phosphate makes vector ends less able to join themselves
Step 1
Concept
Removing 5 prime phosphate can reduce vector self-ligation. This is a strategy to reduce unwanted background in cloning.
Step 2
Why this answer is correct
The correct answer is A. 5 prime phosphate हटने से vector अपने ends आसानी से नहीं जोड़ पाएगा / Removal of 5 prime phosphate makes vector ends less able to join themselves. Removing 5 prime phosphate can reduce vector self-ligation. This is a strategy to reduce unwanted background in cloning.
Step 3
Exam Tip
5 prime phosphate हटने पर vector self-ligation कम हो सकती है। यह cloning में unwanted background घटाने की strategy है।
A. यह vector को insert के बिना बंद कर सकता है/It can close vector without insert
Step 1
Concept
In self-ligation the vector joins its own ends. This does not make the desired recombinant plasmid with insert.
Step 2
Why this answer is correct
The correct answer is A. यह vector को insert के बिना बंद कर सकता है / It can close vector without insert. In self-ligation the vector joins its own ends. This does not make the desired recombinant plasmid with insert.
Step 3
Exam Tip
Self-ligation में vector अपने ही सिरों से जुड़ जाता है। इससे desired insert वाला recombinant plasmid नहीं बनता।
A. कटे vector से पाँच प्राइम फॉस्फेट हटाकर/By removing 5 prime phosphate from cut vector
Step 1
Concept
Removing 5 prime phosphate from vector ends can reduce self-ligation. In exams link phosphate removal with ligation control.
Step 2
Why this answer is correct
The correct answer is A. कटे vector से पाँच प्राइम फॉस्फेट हटाकर / By removing 5 prime phosphate from cut vector. Removing 5 prime phosphate from vector ends can reduce self-ligation. In exams link phosphate removal with ligation control.
Step 3
Exam Tip
Vector ends से पाँच प्राइम फॉस्फेट हटाने पर self-ligation घट सकती है। परीक्षा में phosphate removal को ligation control से जोड़ें।
D. दो अलग restriction enzymes से अलग सिरे बनाना/Making different ends with two restriction enzymes
Step 1
Concept
Two different ends can reduce the chance of vector self-joining. This is useful in directional cloning.
Step 2
Why this answer is correct
The correct answer is D. दो अलग restriction enzymes से अलग सिरे बनाना / Making different ends with two restriction enzymes. Two different ends can reduce the chance of vector self-joining. This is useful in directional cloning.
Step 3
Exam Tip
दो अलग सिरे vector के अपने आप जुड़ने की संभावना घटा सकते हैं। यह directional cloning में उपयोगी है।
C. कटे vector का अपने ही सिरों से जुड़ना/Cut vector joining with its own ends
Step 1
Concept
In self-ligation the vector joins its own ends without insert. This can be an unwanted result in cloning.
Step 2
Why this answer is correct
The correct answer is C. कटे vector का अपने ही सिरों से जुड़ना / Cut vector joining with its own ends. In self-ligation the vector joins its own ends without insert. This can be an unwanted result in cloning.
Step 3
Exam Tip
Self-ligation में vector insert के बिना अपने सिरों से जुड़ जाता है। cloning में यह अनचाहा परिणाम हो सकता है।
A. vector insert के बिना जुड़ सकता है/Vector can join without insert
Step 1
Concept
In self-ligation the vector can join its own ends. This does not form the desired recombinant plasmid.
Step 2
Why this answer is correct
The correct answer is A. vector insert के बिना जुड़ सकता है / Vector can join without insert. In self-ligation the vector can join its own ends. This does not form the desired recombinant plasmid.
Step 3
Exam Tip
Self-ligation में vector अपने ही ends जोड़ सकता है। इससे desired recombinant plasmid नहीं बनता।
A. कटे vector से phosphate हटाने के लिए/To remove phosphate from cut vector
Step 1
Concept
Removing phosphate from vector ends can reduce self-ligation. Correct phosphate ends on insert then help ligation.
Step 2
Why this answer is correct
The correct answer is A. कटे vector से phosphate हटाने के लिए / To remove phosphate from cut vector. Removing phosphate from vector ends can reduce self-ligation. Correct phosphate ends on insert then help ligation.
Step 3
Exam Tip
Vector ends से phosphate हटाने पर self-ligation घट सकती है। फिर insert के सही phosphate ends ligation में मदद करते हैं।
A. दो अलग restriction enzymes से अलग सिरे बनाना/Making different ends with two restriction enzymes
Step 1
Concept
Two different ends can reduce the chance of vector self-joining. This is useful in directional cloning.
Step 2
Why this answer is correct
The correct answer is A. दो अलग restriction enzymes से अलग सिरे बनाना / Making different ends with two restriction enzymes. Two different ends can reduce the chance of vector self-joining. This is useful in directional cloning.
Step 3
Exam Tip
दो अलग ends vector के अपने आप जुड़ने की संभावना घटा सकते हैं। यह directional cloning में उपयोगी है।
A. कटे vector का अपने ही सिरों से जुड़ जाना/Joining of cut vector ends with itself
Step 1
Concept
In self-ligation a vector can join its own ends without insert. This can be an unwanted result in cloning.
Step 2
Why this answer is correct
The correct answer is A. कटे vector का अपने ही सिरों से जुड़ जाना / Joining of cut vector ends with itself. In self-ligation a vector can join its own ends without insert. This can be an unwanted result in cloning.
Step 3
Exam Tip
Self-ligation में vector insert के बिना अपने ही ends से जुड़ सकता है। Cloning में यह unwanted result हो सकता है।
A. दो अलग enzymes से directional cloning/Directional cloning with two different enzymes
Step 1
Concept
Two different incompatible ends can reduce vector self-ligation. This is useful in cloning strategy.
Step 2
Why this answer is correct
The correct answer is A. दो अलग enzymes से directional cloning / Directional cloning with two different enzymes. Two different incompatible ends can reduce vector self-ligation. This is useful in cloning strategy.
Step 3
Exam Tip
दो अलग incompatible ends vector की self-ligation घटा सकते हैं। यह cloning strategy में उपयोगी है।
