Restriction digestion में ends compatible हों लेकिन ligation fail हो तो क्या कारण हो सकता है?
If ends are compatible but ligation fails in restriction digestion work what can be a cause?
Explanation opens after your attempt
A. contaminants या wrong DNA ratioContaminants or wrong DNA ratio
Concept
Besides compatible ends clean DNA and correct ratio are necessary. Salt ethanol or enzyme carryover can block ligation.
Why this answer is correct
The correct answer is A. contaminants या wrong DNA ratio / Contaminants or wrong DNA ratio. Besides compatible ends clean DNA and correct ratio are necessary. Salt ethanol or enzyme carryover can block ligation.
Exam Tip
Compatible ends के अलावा clean DNA और सही ratio जरूरी है। Salt ethanol या enzyme carryover ligation रोक सकते हैं।
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