Concept-wise Practice

blunt end cloning MCQ Questions for Class 11

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Practice Questions

2 questions tagged with blunt end cloning.

Restriction digestion के बाद blunt-end cloning में कौन सा factor अधिक critical हो सकता है?

Which factor can be more critical in blunt-end cloning after restriction digestion?

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Correct Answer

A. DNA concentration और ligation conditionDNA concentration and ligation condition

Step 1

Concept

Blunt ends lack complementary overhang. Therefore concentration and ligation condition become more important.

Step 2

Why this answer is correct

The correct answer is A. DNA concentration और ligation condition / DNA concentration and ligation condition. Blunt ends lack complementary overhang. Therefore concentration and ligation condition become more important.

Step 3

Exam Tip

Blunt ends complementary overhang नहीं रखते। इसलिए concentration और ligation condition अधिक महत्वपूर्ण हो जाते हैं।

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T4 DNA ligase किस वजह से blunt-end cloning में भी उपयोगी है?

Why is T4 DNA ligase useful in blunt-end cloning too?

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Correct Answer

A. यह blunt DNA ends को भी जोड़ सकता हैIt can also join blunt DNA ends

Step 1

Concept

T4 DNA ligase can join both sticky and blunt ends. In blunt-end ligation conditions are kept more carefully.

Step 2

Why this answer is correct

The correct answer is A. यह blunt DNA ends को भी जोड़ सकता है / It can also join blunt DNA ends. T4 DNA ligase can join both sticky and blunt ends. In blunt-end ligation conditions are kept more carefully.

Step 3

Exam Tip

T4 DNA ligase sticky और blunt दोनों ends जोड़ सकता है। Blunt-end ligation में conditions अधिक सावधानी से रखी जाती हैं।

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