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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 271/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion में clean sharp bands क्या संकेत दे सकते हैं?

What can clean sharp bands indicate in restriction digestion?

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A. good DNA quality और proper digestionGood DNA quality and proper digestion

Explanation

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Sharp expected bands clean DNA और correct digestion का संकेत दे सकते हैं। फिर भी map से compare करना जरूरी है। / Sharp expected bands can indicate clean DNA and correct digestion. Still comparison with map is necessary.

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Question 272/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction enzyme reaction के लिए DNA purity का कौन सा issue सबसे problematic हो सकता है?

Which DNA purity issue can be very problematic for restriction enzyme reaction?

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A. phenol ethanol या salt carryoverPhenol ethanol or salt carryover

Explanation

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Phenol ethanol और salts enzyme activity inhibit कर सकते हैं। Digestion से पहले cleanup useful हो सकता है। / Phenol ethanol and salts can inhibit enzyme activity. Cleanup can be useful before digestion.

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Question 273/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion के लिए DNA quantification क्यों किया जाता है?

Why is DNA quantification done for restriction digestion?

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A. enzyme और DNA amount सही रखने के लिएTo keep enzyme and DNA amount correct

Explanation

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DNA amount जानकर enzyme units और reaction setup सही किया जाता है। इससे digestion reliable होती है। / Knowing DNA amount helps set enzyme units and reaction correctly. This makes digestion reliable.

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Question 274/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction enzyme unit क्यों important है?

Why is restriction enzyme unit important?

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A. enzyme activity amount समझने के लिएTo understand enzyme activity amount

Explanation

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Enzyme units बताते हैं कि defined conditions में enzyme कितना DNA काट सकता है। Reaction setup में यह useful है। / Enzyme units show how much DNA the enzyme can cut under defined conditions. This is useful in reaction setup.

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Question 275/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion में too little enzyme का result क्या हो सकता है?

What can be the result of too little enzyme in restriction digestion?

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A. partial digestion

Explanation

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कम enzyme सभी sites नहीं काट पाएगा। इससे incomplete या partial digest हो सकता है। / Too little enzyme may not cut all sites. This can cause incomplete or partial digest.

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Question 276/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion में too much enzyme का risk क्या है?

What is the risk of too much enzyme in restriction digestion?

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A. star activity या glycerol effectStar activity or glycerol effect

Explanation

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Excess enzyme और glycerol specificity को प्रभावित कर सकते हैं। Recommended enzyme amount रखें। / Excess enzyme and glycerol can affect specificity. Use recommended enzyme amount.

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Question 277/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion में optimum temperature क्यों follow करते हैं?

Why is optimum temperature followed in restriction digestion?

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A. enzyme activity best रखने के लिएTo keep enzyme activity best

Explanation

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Enzymes specific temperature range में best काम करते हैं। Wrong temperature digestion fail कर सकता है। / Enzymes work best in a specific temperature range. Wrong temperature can make digestion fail.

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Question 278/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion में buffer का pH क्यों important है?

Why is buffer pH important in restriction digestion?

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A. enzyme structure और activity support करने के लिएTo support enzyme structure and activity

Explanation

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Enzyme activity pH से प्रभावित होती है। Correct buffer pH reaction success में मदद करता है। / Enzyme activity is affected by pH. Correct buffer pH helps reaction success.

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Question 279/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion में nuclease-free water क्यों जरूरी है?

Why is nuclease-free water necessary in restriction digestion?

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A. unwanted DNA degradation रोकने के लिएTo prevent unwanted DNA degradation

Explanation

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Nuclease contamination DNA को unwanted fragments में तोड़ सकती है। Nuclease-free water sample बचाता है। / Nuclease contamination can break DNA into unwanted fragments. Nuclease-free water protects the sample.

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Question 280/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion में fresh sterile tips क्यों use करते हैं?

Why are fresh sterile tips used in restriction digestion?

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A. contamination और sample mix-up कम करने के लिएTo reduce contamination and sample mix-up

Explanation

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Fresh tips nucleases और cross-contamination से बचाते हैं। यह clean reaction के लिए जरूरी है। / Fresh tips avoid nucleases and cross-contamination. This is needed for a clean reaction.

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