Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 261-270 of 592 questions.
What forms when circular DNA has one cut site?
A. linear DNA molecule
Circular DNA एक जगह कटकर linear form बन जाता है। यह plasmid linearization है। / Circular DNA cut at one site becomes a linear form. This is plasmid linearization.
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What is generally obtained when a circular plasmid has two cut sites?
A. दो DNA fragmentsTwo DNA fragments
Circular DNA पर two cuts दो fragments बनाते हैं। यह insert release में useful हो सकता है। / Two cuts on circular DNA make two fragments. This can be useful in insert release.
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Why is fragment count important in restriction digest?
A. cut pattern verify करने के लिएTo verify cut pattern
Fragment count expected map से match होना चाहिए। इससे digestion और construct verification होती है। / Fragment count should match the expected map. This verifies digestion and construct.
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Agarose gel electrophoresis separates DNA fragments on what basis?
A. size
DNA fragments gel में size के अनुसार migrate करते हैं। छोटे fragments सामान्यतः तेज चलते हैं। / DNA fragments migrate according to size in gel. Smaller fragments generally move faster.
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Toward which electrode does DNA move in a gel?
A. positive electrode
DNA phosphate backbone के कारण negatively charged होता है। इसलिए वह positive electrode की ओर जाता है। / DNA is negatively charged due to phosphate backbone. Therefore it moves toward positive electrode.
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What is the simplest purpose of a DNA ladder?
A. fragment size estimate करनाEstimating fragment size
DNA ladder known size bands देता है। इससे sample fragments का approximate size निकाला जाता है। / DNA ladder gives known size bands. It helps estimate approximate size of sample fragments.
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If expected band is absent in restriction digest gel what can be a first possibility?
A. incomplete digestion या wrong cloneIncomplete digestion or wrong clone
Expected band absent होना digestion failure wrong clone या low DNA दिखा सकता है। Controls check करें। / Absence of expected band can show digestion failure wrong clone or low DNA. Check controls.
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What can be a common reason for very faint bands?
A. low DNA amount
कम DNA loading faint bands दे सकती है। Sample concentration और loading amount देखें। / Low DNA loading can give faint bands. Check sample concentration and loading amount.
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What can be one cause of extra bands?
A. partial digestion या star activityPartial digestion or star activity
Extra bands incomplete cutting या non-specific cutting का संकेत दे सकते हैं। Reaction conditions check करें। / Extra bands can indicate incomplete cutting or non-specific cutting. Check reaction conditions.
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What is one likely reason for smear in restriction digest?
A. degraded DNA
Degraded DNA many fragment sizes देता है जो smear दिखा सकते हैं। DNA quality check करें। / Degraded DNA gives many fragment sizes that can appear as smear. Check DNA quality.
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