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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 281/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion में sample mix-up रोकने का basic तरीका क्या है?

What is the basic way to prevent sample mix-up in restriction digestion?

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A. clear labelling और lane mapClear labelling and lane map

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Clear labels reactions को identify करते हैं और lane map gel interpretation बचाता है। यह record keeping का part है। / Clear labels identify reactions and lane map protects gel interpretation. This is part of record keeping.

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Question 282/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion में documentation में कौन सी details होनी चाहिए?

Which details should be in documentation for restriction digestion?

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A. enzyme buffer time temperature expected bands observed bands

Explanation

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Complete documentation repeat experiment और troubleshooting में मदद करती है। Expected और observed bands compare करें। / Complete documentation helps repeat experiment and troubleshooting. Compare expected and observed bands.

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Question 283/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion में sequencing कब useful होती है?

When is sequencing useful in restriction digestion work?

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A. final clone sequence confirm करने के लिएTo confirm final clone sequence

Explanation

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Restriction digest size pattern देता है पर exact base sequence नहीं बताता। Sequencing final confirmation देती है। / Restriction digest gives size pattern but not exact base sequence. Sequencing gives final confirmation.

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Question 284/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digest और ligation में direct connection क्या है?

What is the direct connection between restriction digest and ligation?

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A. digest compatible ends बनाता है जिन्हें ligase जोड़ता हैDigest makes compatible ends that ligase joins

Explanation

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Cutting के बाद DNA ends तयार होते हैं। Ligase उन्हीं ends को जोड़कर recombinant DNA बनाता है। / After cutting DNA ends are prepared. Ligase joins those ends to make recombinant DNA.

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Question 285/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion के बाद transformation से पहले कौन सा step आता है?

Which step comes after restriction digestion before transformation?

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A. ligation या recombinant DNA formationLigation or recombinant DNA formation

Explanation

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DNA fragments को ligase से जोड़कर recombinant DNA बनाया जाता है। फिर इसे host में introduce किया जाता है। / DNA fragments are joined by ligase to make recombinant DNA. Then it is introduced into a host.

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Question 286/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Cutting of DNA में vector और insert दोनों क्यों काटे जाते हैं?

Why are both vector and insert cut in Cutting of DNA?

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A. compatible ends पाने के लिएTo obtain compatible ends

Explanation

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Vector और insert compatible ends रखते हैं तो ligation आसान होती है। यह recombinant DNA formation का आधार है। / When vector and insert have compatible ends ligation is easier. This is the basis of recombinant DNA formation.

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Question 287/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion में vector backbone को preserve करना क्यों जरूरी है?

Why is preserving vector backbone important in restriction digestion?

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A. origin और marker जैसे जरूरी elements बचाने के लिएTo keep essential elements like origin and marker

Explanation

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Vector backbone में replication origin और selectable marker होते हैं। गलत cutting vector function खराब कर सकती है। / Vector backbone contains replication origin and selectable marker. Wrong cutting can damage vector function.

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Question 288/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion में insert integrity क्यों जरूरी है?

Why is insert integrity necessary in restriction digestion?

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A. desired gene function बचाने के लिएTo preserve desired gene function

Explanation

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अगर insert unwanted cut हो जाए तो desired gene function खो सकता है। Internal sites check करें। / If the insert is cut unwantedly desired gene function can be lost. Check internal sites.

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Question 289/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion की success का सबसे simple visual proof क्या है?

What is the simplest visual proof of restriction digestion success?

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A. gel पर expected DNA band patternExpected DNA band pattern on gel

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Expected band pattern digestion success का visual evidence देता है। Compare with ladder और controls। / Expected band pattern gives visual evidence of digestion success. Compare with ladder and controls.

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Question 290/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Cutting of DNA में सबसे common student mistake क्या हो सकती है?

What can be a common student mistake in Cutting of DNA?

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A. enzyme और recognition site को match न करनाNot matching enzyme and recognition site

Explanation

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Restriction enzyme तभी cut करेगा जब उसका recognition site मौजूद हो। Enzyme-site matching हमेशा check करें। / A restriction enzyme cuts only if its recognition site is present. Always check enzyme-site matching.

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