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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

Cutting of DNA Practice Questions

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Question 241/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Level 49 में cloning success का main practical factor क्या है?

What is the main practical factor for cloning success in Level 49?

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A. correct enzyme choice and clean compatible ends

Explanation

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Correct cutting insert और vector को सही तरह जोड़ने योग्य बनाता है। Cleanup और ratio ligation को support करते हैं। / Correct cutting makes insert and vector suitable for joining. Cleanup and ratio support ligation.

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Question 242/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Cutting of DNA level 49 का final summary क्या है?

What is the final summary of Cutting of DNA level 49?

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A. restriction cutting में compatible ends बनाकर ligation और verification के लिए DNA तैयार होता हैRestriction cutting prepares DNA for ligation and verification by forming compatible ends

Explanation

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DNA cutting का goal controlled fragments और ends बनाना है। Accurate setup gel analysis और records result को reliable बनाते हैं। / The goal of DNA cutting is to make controlled fragments and ends. Accurate setup gel analysis and records make results reliable.

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Question 243/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction digestion में सबसे पहले कौन सा question पूछना चाहिए?

What question should be asked first in restriction digestion?

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A. मुझे DNA कहाँ cut करना हैWhere do I need to cut DNA

Explanation

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Cutting goal साफ होने पर enzyme choice सही होती है। Cloning planning का पहला step target site समझना है। / When the cutting goal is clear enzyme choice becomes correct. The first step in cloning planning is understanding target site.

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Question 244/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Cutting of DNA में enzyme selection का सबसे बड़ा आधार क्या है?

What is the biggest basis of enzyme selection in Cutting of DNA?

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A. recognition site और desired endsRecognition site and desired ends

Explanation

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Enzyme वही चुना जाता है जिसका recognition site useful हो और ends cloning के लिए suitable हों। / The enzyme is chosen when its recognition site is useful and ends are suitable for cloning.

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Question 245/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction enzyme DNA में random cutting क्यों नहीं करता?

Why does a restriction enzyme not cut DNA randomly?

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A. क्योंकि वह specific recognition sequence पहचानता हैBecause it recognizes a specific recognition sequence

Explanation

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Restriction enzymes specific sequences पर bind करते हैं। इसलिए cut pattern predictable होता है। / Restriction enzymes bind to specific sequences. Therefore the cut pattern is predictable.

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Question 246/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Predictable cutting recombinant DNA technology में क्यों जरूरी है?

Why is predictable cutting necessary in recombinant DNA technology?

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A. planned fragments और compatible ends पाने के लिएTo obtain planned fragments and compatible ends

Explanation

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Recombinant DNA में insert और vector को planned way में तैयार करना होता है। Predictable cutting cloning success बढ़ाती है। / In recombinant DNA insert and vector must be prepared in a planned way. Predictable cutting increases cloning success.

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Question 247/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction enzyme reaction का target molecule क्या है?

What is the target molecule of a restriction enzyme reaction?

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A. DNA

Explanation

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Restriction enzymes DNA substrate पर काम करते हैं। वे DNA backbone के phosphodiester bonds काटते हैं। / Restriction enzymes act on DNA substrate. They cut phosphodiester bonds in the DNA backbone.

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Question 248/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

DNA backbone cutting किस bond से संबंधित है?

DNA backbone cutting is related to which bond?

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A. phosphodiester bond

Explanation

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DNA backbone phosphodiester bonds से बना होता है। Restriction enzymes इन्हीं bonds को काटते हैं। / DNA backbone is made of phosphodiester bonds. Restriction enzymes cut these bonds.

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Question 249/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Restriction enzyme को endonuclease क्यों कहा जाता है?

Why is a restriction enzyme called an endonuclease?

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A. क्योंकि यह nucleic acid chain के अंदर cut करता हैBecause it cuts inside a nucleic acid chain

Explanation

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Endonuclease nucleic acid के internal phosphodiester bonds काटता है। Restriction enzymes इसी group में हैं। / An endonuclease cuts internal phosphodiester bonds in nucleic acids. Restriction enzymes are in this group.

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Question 250/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 50

Exonuclease और restriction endonuclease में मुख्य अंतर क्या है?

What is the main difference between exonuclease and restriction endonuclease?

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A. Exonuclease ends से हटाता है और restriction endonuclease अंदर specific site काटता हैExonuclease removes from ends and restriction endonuclease cuts internal specific site

Explanation

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Restriction endonuclease specific internal sites काटता है। Exonuclease nucleotides ends से remove करता है। / Restriction endonuclease cuts specific internal sites. Exonuclease removes nucleotides from ends.

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