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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 221/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction cutting के बाद ligation से पहले DNA ends की cleanliness क्यों जरूरी है?

Why is cleanliness of DNA ends important before ligation after restriction cutting?

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A. contaminants ligase activity रोक सकते हैंContaminants can inhibit ligase activity

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Salt ethanol phenol या enzymes ligation में बाधा डाल सकते हैं। Clean DNA ends बेहतर joining देते हैं। / Salt ethanol phenol or enzymes can interfere with ligation. Clean DNA ends give better joining.

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Question 222/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction digest में clean DNA sample क्यों जरूरी है?

Why is a clean DNA sample necessary in restriction digest?

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A. restriction enzyme impurities से inhibit हो सकता हैRestriction enzyme can be inhibited by impurities

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Phenol ethanol और salts enzyme activity कम कर सकते हैं। इसलिए digestion से पहले DNA purity check करें। / Phenol ethanol and salts can reduce enzyme activity. Therefore check DNA purity before digestion.

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Question 223/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

DNA isolation से आए EDTA का restriction digestion पर क्या असर हो सकता है?

What effect can EDTA from DNA isolation have on restriction digestion?

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A. magnesium bind कर enzyme activity घटा सकता हैIt can bind magnesium and reduce enzyme activity

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EDTA divalent ions bind करता है। कई restriction enzymes को magnesium चाहिए होता है। / EDTA binds divalent ions. Many restriction enzymes need magnesium.

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Question 224/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

TE buffer में रखे DNA को digestion में use करते समय क्या ध्यान दें?

What should be considered when using DNA stored in TE buffer for digestion?

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A. EDTA concentration enzyme reaction को affect कर सकती हैEDTA concentration can affect enzyme reaction

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TE में EDTA होता है जो magnesium chelate कर सकता है। Reaction setup में buffer compatibility देखें। / TE contains EDTA that can chelate magnesium. Check buffer compatibility in reaction setup.

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Question 225/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction digestion में salt contamination कहाँ से आ सकती है?

Where can salt contamination come from in restriction digestion?

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A. DNA purification wash carryover सेFrom DNA purification wash carryover

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Purification से leftover salts enzyme activity बदल सकते हैं। DNA cleanup और proper drying helpful हैं। / Leftover salts from purification can alter enzyme activity. DNA cleanup and proper drying are helpful.

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Question 226/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction digestion में reaction volume बहुत छोटा रखने से क्या practical समस्या हो सकती है?

What practical problem can occur if reaction volume is kept too small in restriction digestion?

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A. pipetting error का effect अधिक हो सकता हैPipetting error effect can be larger

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बहुत छोटे volumes में small pipetting errors भी concentration बदल सकते हैं। Accurate pipetting जरूरी है। / In very small volumes small pipetting errors can change concentrations. Accurate pipetting is necessary.

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Question 227/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction digestion master mix का एक benefit क्या है?

What is one benefit of a restriction digestion master mix?

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A. common components समान रखने में मददHelps keep common components the same

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Master mix pipetting variation कम करता है। Multiple reactions में reproducibility बेहतर होती है। / A master mix reduces pipetting variation. Reproducibility improves in multiple reactions.

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Question 228/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction digest setup में bubbles क्यों avoid करते हैं?

Why are bubbles avoided in restriction digest setup?

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A. accurate mixing और pipetting maintain करने के लिएTo maintain accurate mixing and pipetting

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Bubbles small reaction volumes में inaccurate volume और mixing दे सकते हैं। Gentle pipetting बेहतर है। / Bubbles can cause inaccurate volume and mixing in small reactions. Gentle pipetting is better.

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Question 229/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction digestion में tube spin-down क्यों किया जाता है?

Why is a tube spin-down done in restriction digestion?

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A. liquid को bottom पर collect करने के लिएTo collect liquid at the bottom

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Spin-down droplets को tube wall से नीचे लाता है। इससे reaction components properly mix होते हैं। / Spin-down brings droplets from tube wall to bottom. This helps reaction components mix properly.

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Question 230/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction digestion में evaporation रोकना क्यों जरूरी है?

Why is preventing evaporation important in restriction digestion?

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A. reaction concentration stable रखने के लिएTo keep reaction concentration stable

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Evaporation से salt और enzyme concentrations बदल सकती हैं। Proper tube closure और incubation conditions important हैं। / Evaporation can change salt and enzyme concentrations. Proper tube closure and incubation conditions are important.

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