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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 211/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

अगर double digest में एक enzyme ही काम करे तो gel पर क्या हो सकता है?

If only one enzyme works in double digest what can happen on gel?

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A. single digest जैसा pattern आ सकता हैA single-digest-like pattern can appear

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One enzyme failure expected double digest pattern नहीं देगा। Controls help identify which enzyme failed। / One enzyme failure will not give expected double digest pattern. Controls help identify which enzyme failed.

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Question 212/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Double digest troubleshooting में single enzyme controls क्यों useful हैं?

Why are single enzyme controls useful in double digest troubleshooting?

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A. कौन सा enzyme काम कर रहा है पहचानने के लिएTo identify which enzyme is working

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Single controls each enzyme activity को अलग से दिखाते हैं। इससे failure source पता चलता है। / Single controls show activity of each enzyme separately. This reveals the failure source.

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Question 213/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction digestion में enzyme volume बहुत ज्यादा क्यों avoid करते हैं?

Why is too much enzyme volume avoided in restriction digestion?

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A. storage glycerol ज्यादा होकर star activity बढ़ सकती हैStorage glycerol can become high and increase star activity

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Enzyme storage buffer में glycerol होता है। Reaction में अधिक glycerol specificity घटा सकता है। / Enzyme storage buffer contains glycerol. Too much glycerol in reaction can reduce specificity.

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Question 214/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction digestion के बाद enzyme activity बची रहे तो next cloning step में क्या risk है?

If enzyme activity remains after restriction digestion what is the risk in next cloning step?

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A. unwanted further cutting हो सकती हैUnwanted further cutting can occur

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Active enzyme ligation mix में DNA ends को फिर काट सकता है। Cleanup या inactivation useful हो सकती है। / Active enzyme can cut DNA ends again in ligation mix. Cleanup or inactivation can be useful.

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Question 215/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction digestion cleanup के बाद DNA concentration फिर क्यों मापी जाती है?

Why is DNA concentration measured again after restriction digestion cleanup?

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A. cleanup में DNA loss हो सकता हैDNA loss can occur during cleanup

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Cleanup recovery पूर्ण नहीं होती। Ligation setup के लिए actual DNA concentration जानना जरूरी है। / Cleanup recovery is not complete. Actual DNA concentration is needed for ligation setup.

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Question 216/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Insert to vector molar ratio क्यों important है?

Why is insert to vector molar ratio important?

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A. ligation product formation को प्रभावित करता हैIt affects ligation product formation

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Insert और vector का सही ratio recombinant clones की संभावना बढ़ा सकता है। Incorrect ratio low cloning efficiency दे सकता है। / Correct insert and vector ratio can increase chances of recombinant clones. Incorrect ratio can give low cloning efficiency.

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Question 217/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Vector dephosphorylation का उद्देश्य क्या है?

What is the purpose of vector dephosphorylation?

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A. vector self-ligation घटानाReducing vector self-ligation

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Phosphate हटाने से vector ends अपने आप जुड़ने की संभावना कम होती है। इससे recombinant clone proportion बढ़ सकता है। / Removing phosphate reduces the chance of vector ends joining themselves. This can increase recombinant clone proportion.

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Question 218/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Alkaline phosphatase treatment के बाद cleanup क्यों जरूरी हो सकता है?

Why can cleanup be needed after alkaline phosphatase treatment?

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A. phosphatase हटाने के लिए जो ligation को affect कर सकती हैTo remove phosphatase that can affect ligation

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Residual phosphatase ligation setup में phosphate removal जारी रख सकती है। इसलिए cleanup useful है। / Residual phosphatase can keep removing phosphate in ligation setup. Therefore cleanup is useful.

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Question 219/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction enzyme से cut vector का self-ligation कब अधिक हो सकता है?

When can self-ligation of a restriction-cut vector be more likely?

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A. single enzyme sticky ends compatible होंSingle enzyme sticky ends are compatible

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Same compatible ends vector को खुद से ligate कर सकते हैं। Dephosphorylation या directional cloning इसे घटा सकती है। / Same compatible ends can let vector ligate to itself. Dephosphorylation or directional cloning can reduce this.

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Question 220/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Directional cloning self-ligation को कैसे कम कर सकती है?

How can directional cloning reduce self-ligation?

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A. दो अलग incompatible vector ends बनाकरBy creating two different incompatible vector ends

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Different ends vector को same orientation में close होने से रोक सकते हैं। Insert correct orientation में लग सकता है। / Different ends can prevent the vector from closing in the same orientation. Insert can join in correct orientation.

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