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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 191/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Level 48 का best revision chain क्या है?

What is the best revision chain for Level 48?

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A. enzyme choice map digestion gel comparison troubleshooting

Explanation

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Cutting of DNA में enzyme choice से gel interpretation तक पूरी chain समझनी चाहिए। Controls result को reliable बनाते हैं। / In Cutting of DNA the full chain from enzyme choice to gel interpretation should be understood. Controls make results reliable.

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Question 192/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Cutting of DNA level 48 का final summary क्या है?

What is the final summary of Cutting of DNA level 48?

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A. सही enzyme और condition से DNA predictable fragments में कटता है और gel से verify होता हैWith correct enzyme and condition DNA cuts into predictable fragments and is verified by gel

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Restriction digestion cloning planning और verification दोनों में important है। Accurate maps controls और records जरूरी हैं। / Restriction digestion is important in both cloning planning and verification. Accurate maps controls and records are needed.

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Question 193/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction digestion में compatible sticky ends क्यों useful होते हैं?

Why are compatible sticky ends useful in restriction digestion?

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A. वे complementary base pairing कर सकते हैंThey can do complementary base pairing

Explanation

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Compatible sticky ends temporary pairing करते हैं। Ligase बाद में backbone को स्थायी रूप से जोड़ता है। / Compatible sticky ends pair temporarily. Ligase later joins the backbone permanently.

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Question 194/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Cohesive ends किसके दूसरे नाम हैं?

Cohesive ends are another name for what?

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B. sticky ends

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Sticky ends complementary overhangs के कारण cohesive ends भी कहलाते हैं। वे ligation में मदद करते हैं। / Sticky ends are also called cohesive ends because of complementary overhangs. They help in ligation.

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Question 195/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Blunt ends ligation में relatively कठिन क्यों हो सकते हैं?

Why can blunt ends be relatively difficult in ligation?

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A. उनमें complementary overhang नहीं होताThey lack complementary overhang

Explanation

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Blunt ends में sticky base pairing support नहीं होता। इसलिए ligation efficiency कम हो सकती है। / Blunt ends lack sticky base pairing support. Therefore ligation efficiency can be lower.

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Question 196/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

किस cut से 5 prime overhang बन सकता है?

Which cut can produce a 5 prime overhang?

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A. staggered cut

Explanation

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Staggered cuts single-stranded overhangs बना सकते हैं। Overhang 5 prime या 3 prime हो सकता है। / Staggered cuts can produce single-stranded overhangs. The overhang can be 5 prime or 3 prime.

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Question 197/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

3 prime overhang का अर्थ क्या है?

What does a 3 prime overhang mean?

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A. single-stranded extension 3 prime end पर हैSingle-stranded extension is at the 3 prime end

Explanation

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कुछ restriction enzymes 3 prime overhang बनाते हैं। Overhang type ligation compatibility को प्रभावित करता है। / Some restriction enzymes produce 3 prime overhangs. Overhang type affects ligation compatibility.

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Question 198/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction enzyme से बने ends की polarity जानना क्यों जरूरी है?

Why is it important to know polarity of ends produced by restriction enzyme?

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A. compatible ligation plan बनाने के लिएTo make a compatible ligation plan

Explanation

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5 prime और 3 prime overhangs अलग compatibility रखते हैं। Cloning design में end polarity important है। / 5 prime and 3 prime overhangs have different compatibility. End polarity is important in cloning design.

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Question 199/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Same overhang देने वाले अलग enzymes cloning में कैसे useful हो सकते हैं?

How can different enzymes giving the same overhang be useful in cloning?

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A. compatible ligation allow कर सकते हैंThey can allow compatible ligation

Explanation

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Different enzymes sometimes same overhang बना सकते हैं। ऐसे ends ligate हो सकते हैं। / Different enzymes can sometimes produce the same overhang. Such ends can ligate.

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Question 200/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Compatible ends ligate होने के बाद recognition site हमेशा वापस क्यों नहीं बनती?

Why is the recognition site not always restored after compatible ends ligate?

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A. क्योंकि अलग enzymes के ends जुड़ने पर hybrid site बन सकती हैBecause ends from different enzymes can form a hybrid site

Explanation

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Compatible ends from different enzymes ligate होकर original sites बदल सकते हैं। इससे site दुबारा cut न भी हो सके। / Compatible ends from different enzymes can ligate and change original sites. The site may not cut again.

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