Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 171-180 of 592 questions.
What is the risk of keeping DNA band on UV transilluminator for too long?
A. DNA damage
UV light DNA को damage कर सकती है। Band को जल्दी cut करना बेहतर है। / UV light can damage DNA. It is better to cut the band quickly.
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What can be an advantage of a blue light transilluminator?
A. UV damage कम हो सकता हैUV damage can be reduced
Blue light DNA damage को UV की तुलना में कम कर सकता है। यह cloning fragments के लिए helpful है। / Blue light can reduce DNA damage compared with UV. It is helpful for cloning fragments.
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Why is gel loading dye added in restriction digestion analysis?
A. sample loading आसान करने और migration track करने के लिएTo ease sample loading and track migration
Loading dye sample को well में बैठाने और run track करने में मदद करता है। यह DNA काटता नहीं है। / Loading dye helps sample sink into wells and track the run. It does not cut DNA.
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What is considered while choosing agarose gel percentage?
A. expected fragment size range
Different agarose percentages different size ranges को better separate करते हैं। Fragment size के अनुसार gel चुनें। / Different agarose percentages separate different size ranges better. Choose gel according to fragment size.
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Why can higher agarose percentage be useful for small DNA fragments?
A. better resolution के लिएFor better resolution
Higher agarose gel छोटे fragments को बेहतर अलग कर सकता है। यह size resolution बढ़ाता है। / Higher agarose gel can separate small fragments better. It improves size resolution.
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Why can lower agarose percentage help for large DNA fragments?
A. large fragments migration आसान करने के लिएTo allow easier migration of large fragments
Lower agarose gel में pores बड़े होते हैं। इससे large fragments better resolve हो सकते हैं। / Lower agarose gel has larger pores. This can help resolve large fragments better.
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Why is the ladder lane necessary on a restriction digest gel?
A. fragment size compare करने के लिएTo compare fragment sizes
DNA ladder known fragment sizes देता है। इसके बिना sample band size estimate कठिन होता है। / DNA ladder gives known fragment sizes. Without it estimating sample band size is difficult.
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Why can different forms of the same DNA appear in restriction enzyme result?
A. plasmid conformation जैसे supercoiled open circular और linear के कारणDue to plasmid conformations like supercoiled open circular and linear
Uncut plasmid अलग conformations में migrate कर सकता है। Digest के बाद linear band अलग दिखती है। / Uncut plasmid can migrate in different conformations. After digestion the linear band appears differently.
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Why can an open circular plasmid migrate differently on a gel?
A. उसकी conformation compact नहीं होतीIts conformation is not compact
Open circular plasmid supercoiled form से कम compact होता है। इसलिए gel mobility अलग होती है। / Open circular plasmid is less compact than supercoiled form. Therefore gel mobility differs.
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Why does supercoiled plasmid often travel a different distance than linear DNA?
A. compact shape migration बदलती हैCompact shape changes migration
Plasmid shape gel mobility को प्रभावित करती है। इसलिए uncut plasmid size estimate सावधानी से करें। / Plasmid shape affects gel mobility. Therefore estimate uncut plasmid size carefully.
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