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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 151/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Restriction digestion में positive control किसलिए उपयोगी है?

Why is a positive control useful in restriction digestion?

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A. enzyme activity verify करने के लिएTo verify enzyme activity

Explanation

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Known DNA पर expected cut enzyme की working दिखाता है। Positive control failure reaction problem बताता है। / Expected cutting on known DNA shows enzyme working. Positive control failure indicates reaction problem.

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Question 152/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

No-enzyme control का सही उपयोग क्या है?

What is the correct use of a no-enzyme control?

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B. uncut DNA pattern दिखाने के लिएTo show uncut DNA pattern

Explanation

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No-enzyme control से original DNA form दिखती है। इससे cut sample के pattern की तुलना होती है। / A no-enzyme control shows the original DNA form. This is compared with the cut sample pattern.

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Question 153/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

अगर no-enzyme control में भी smear दिखे तो क्या संकेत हो सकता है?

If smear appears even in no-enzyme control what can it indicate?

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B. DNA पहले से degraded हो सकता हैDNA may already be degraded

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No-enzyme lane में smear DNA quality problem दिखा सकता है। Enzyme को दोष देने से पहले DNA integrity check करें। / A smear in no-enzyme lane can show DNA quality problem. Check DNA integrity before blaming the enzyme.

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Question 154/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Restriction enzyme reaction में correct buffer क्यों जरूरी है?

Why is correct buffer necessary in restriction enzyme reaction?

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A. enzyme के pH salt और cofactors को सही रखने के लिएTo provide correct pH salt and cofactors for enzyme

Explanation

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Restriction enzymes defined buffer conditions में best काम करते हैं। गलत buffer incomplete digestion दे सकता है। / Restriction enzymes work best in defined buffer conditions. Wrong buffer can give incomplete digestion.

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Question 155/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

कुछ restriction enzymes को magnesium ion क्यों चाहिए होता है?

Why do some restriction enzymes need magnesium ion?

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A. enzyme activity के cofactor के रूप मेंAs a cofactor for enzyme activity

Explanation

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Magnesium ion कई restriction enzymes की catalytic activity में मदद करता है। Buffer में required ions दिए जाते हैं। / Magnesium ion helps catalytic activity of many restriction enzymes. Required ions are provided in buffer.

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Question 156/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Restriction digestion में enzyme को reaction setup के दौरान ठंडा क्यों रखा जाता है?

Why is the enzyme kept cold during restriction digestion setup?

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A. enzyme stability बनाए रखने के लिएTo maintain enzyme stability

Explanation

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Restriction enzymes heat-sensitive proteins हो सकते हैं। Cold handling activity loss कम करता है। / Restriction enzymes can be heat-sensitive proteins. Cold handling reduces activity loss.

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Question 157/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Reaction mix में enzyme last में add करने का practical कारण क्या है?

What is the practical reason for adding enzyme last in reaction mix?

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A. enzyme exposure और timing control करनाTo control enzyme exposure and timing

Explanation

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बाकी components पहले सही concentration में मिल जाते हैं। Enzyme last में डालने से reaction timing better control होती है। / Other components are mixed at correct concentration first. Adding enzyme last gives better reaction timing control.

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Question 158/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Restriction digestion में बहुत अधिक DNA डालने से क्या हो सकता है?

What can happen if too much DNA is added in restriction digestion?

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A. enzyme insufficient होकर incomplete digestion दे सकता हैEnzyme may become insufficient and give incomplete digestion

Explanation

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DNA amount enzyme capacity से match होना चाहिए। बहुत अधिक DNA complete digestion को कठिन कर सकता है। / DNA amount should match enzyme capacity. Too much DNA can make complete digestion difficult.

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Question 159/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Reaction में बहुत कम DNA होने पर gel result कैसा हो सकता है?

What can gel result look like if DNA is too low in a reaction?

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A. band बहुत faint हो सकती हैBand can be very faint

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Low DNA loading faint bands दे सकता है। DNA concentration check करके suitable amount load करें। / Low DNA loading can give faint bands. Check DNA concentration and load a suitable amount.

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Question 160/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Restriction digestion में incubation time बढ़ाने से हमेशा फायदा क्यों नहीं होता?

Why does increasing incubation time not always help in restriction digestion?

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A. बहुत लंबी incubation star activity या DNA damage risk बढ़ा सकती हैVery long incubation can increase star activity or DNA damage risk

Explanation

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Recommended time digestion के लिए enough होता है। Too long incubation specificity घटा सकती है। / Recommended time is enough for digestion. Too long incubation can reduce specificity.

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