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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 161/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Star activity कब अधिक हो सकती है?

When can star activity increase?

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A. wrong buffer excess enzyme या long incubation मेंIn wrong buffer excess enzyme or long incubation

Explanation

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Star activity में enzyme non-specific sites पर cut कर सकता है। Recommended conditions इसे कम करती हैं। / In star activity the enzyme can cut at non-specific sites. Recommended conditions reduce it.

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Question 162/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Partial digestion और star activity में मुख्य अंतर क्या है?

What is the main difference between partial digestion and star activity?

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A. partial में सभी sites नहीं कटते और star में गलत sites भी कट सकते हैंIn partial digestion not all sites cut and in star activity wrong sites may cut

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Partial digestion incomplete cutting है। Star activity reduced specificity के कारण extra cutting दे सकती है। / Partial digestion is incomplete cutting. Star activity can give extra cutting due to reduced specificity.

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Question 163/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

अगर DNA methylated है तो कुछ restriction enzymes क्यों fail हो सकते हैं?

Why can some restriction enzymes fail if DNA is methylated?

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A. methylation recognition या cutting रोक सकती हैMethylation can block recognition or cutting

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कुछ enzymes methylated recognition sites नहीं काटते। इसलिए methylation sensitivity देखना जरूरी है। / Some enzymes do not cut methylated recognition sites. Therefore methylation sensitivity must be checked.

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Question 164/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Dam या Dcm methylation किससे जुड़ी हो सकती है?

Dam or Dcm methylation can be related to what?

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A. bacterial DNA methylation

Explanation

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कुछ bacterial strains DNA को methylate करते हैं। यह restriction digestion patterns को प्रभावित कर सकता है। / Some bacterial strains methylate DNA. This can affect restriction digestion patterns.

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Question 165/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

अगर restriction enzyme methylation-sensitive है तो cloning plan में क्या ध्यान रखें?

If a restriction enzyme is methylation-sensitive what should be considered in cloning plan?

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A. DNA source और methylation statusDNA source and methylation status

Explanation

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Methylated DNA enzyme cutting रोक सकता है। इसलिए suitable host strain या alternative enzyme चुना जा सकता है। / Methylated DNA can block enzyme cutting. Therefore a suitable host strain or alternative enzyme can be chosen.

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Question 166/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Restriction digestion के बाद heat inactivation कब useful है?

When is heat inactivation useful after restriction digestion?

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A. जब enzyme को next step से पहले रोकना होWhen enzyme must be stopped before next step

Explanation

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Heat inactivation कुछ enzymes की activity रोक सकती है। हमेशा enzyme-specific instructions देखें। / Heat inactivation can stop activity of some enzymes. Always check enzyme-specific instructions.

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Question 167/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

सभी restriction enzymes heat-inactivated क्यों नहीं होते?

Why are all restriction enzymes not heat-inactivated?

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A. हर enzyme की heat stability अलग होती हैEach enzyme has different heat stability

Explanation

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कुछ enzymes heat के बाद भी active रह सकते हैं। इसलिए purification की जरूरत हो सकती है। / Some enzymes can remain active after heat. Therefore purification may be needed.

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Question 168/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Restriction digestion cleanup का लाभ क्या है?

What is the benefit of restriction digestion cleanup?

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A. enzyme buffer salt और small fragments हटानाRemoving enzyme buffer salt and small fragments

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Cleanup ligation या PCR जैसे next steps को बेहतर बना सकता है। Clean DNA reactions में अधिक useful होता है। / Cleanup can improve next steps like ligation or PCR. Clean DNA is more useful in reactions.

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Question 169/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Gel extraction कब जरूरी हो सकती है?

When can gel extraction be necessary?

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A. desired DNA fragment को बाकी fragments से अलग करने के लिएTo separate desired DNA fragment from other fragments

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Gel extraction specific band को recover करती है। यह insert purification में useful है। / Gel extraction recovers a specific band. It is useful in insert purification.

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Question 170/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 48

Gel extraction के दौरान DNA loss क्यों हो सकता है?

Why can DNA loss occur during gel extraction?

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A. binding washing या elution में incomplete recovery सेDue to incomplete recovery during binding washing or elution

Explanation

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Gel extraction में DNA recovery पूर्ण नहीं होती। इसलिए band cutting और elution सावधानी से करें। / DNA recovery in gel extraction is not complete. Therefore cut bands and elute carefully.

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