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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 201/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction site destroy होना cloning verification में कब useful हो सकता है?

When can destruction of restriction site be useful in cloning verification?

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A. जब ligation के बाद old site cut न होWhen the old site does not cut after ligation

Explanation

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कुछ ligations original restriction site को खत्म कर देते हैं। Diagnostic digest से यह पता लगाया जा सकता है। / Some ligations destroy the original restriction site. Diagnostic digest can detect this.

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Question 202/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction digestion से insert orientation directly क्यों हर बार confirm नहीं होती?

Why does restriction digestion not always directly confirm insert orientation?

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A. कभी band pattern orientation में समान हो सकता हैSometimes band pattern can be same for orientations

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Orientation confirm करने के लिए informative restriction sites चाहिए। Otherwise sequencing या orientation PCR जरूरी हो सकता है। / Informative restriction sites are needed to confirm orientation. Otherwise sequencing or orientation PCR may be needed.

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Question 203/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Informative diagnostic digest का अर्थ क्या है?

What does informative diagnostic digest mean?

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A. ऐसा digest जो expected alternatives को अलग-अलग band patterns देA digest that gives different band patterns for expected alternatives

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Informative digest सही clone और wrong clone में फर्क दिखाता है। Enzyme selection इसी आधार पर होती है। / An informative digest distinguishes correct clone from wrong clone. Enzyme selection is based on this.

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Question 204/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction map से क्या predict किया जा सकता है?

What can be predicted from a restriction map?

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A. enzyme cut positions और fragment sizesEnzyme cut positions and fragment sizes

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Restriction map expected bands बताता है। यह digestion result की interpretation में मदद करता है। / A restriction map tells expected bands. It helps interpret digestion result.

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Question 205/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction map के बिना diagnostic digest क्यों कठिन हो सकता है?

Why can diagnostic digest be difficult without a restriction map?

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A. expected bands पता नहीं चलेंगेExpected bands will not be known

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Map expected fragment sizes देता है। बिना map observed bands का meaning unclear हो सकता है। / A map gives expected fragment sizes. Without a map observed band meaning can be unclear.

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Question 206/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Virtual digest software किसलिए useful है?

Why is virtual digest software useful?

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A. enzyme sites और bands का prediction करने के लिएTo predict enzyme sites and bands

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Virtual digest actual experiment से पहले expected pattern दिखा सकता है। यह planning में helpful है। / Virtual digest can show expected pattern before the actual experiment. It is helpful in planning.

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Question 207/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction enzyme supplier information क्यों check की जाती है?

Why is restriction enzyme supplier information checked?

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A. buffer temperature methylation sensitivity और heat inactivation जानने के लिएTo know buffer temperature methylation sensitivity and heat inactivation

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हर enzyme की working condition अलग हो सकती है। Official data सही reaction setup में मदद करता है। / Each enzyme can have different working conditions. Official data helps correct reaction setup.

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Question 208/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Restriction enzyme के साथ provided buffer use करना क्यों बेहतर है?

Why is it better to use the buffer provided with the restriction enzyme?

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A. वह enzyme की activity के लिए optimized होता हैIt is optimized for enzyme activity

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Provided buffer pH salt और cofactors को enzyme के अनुसार रखता है। इससे digestion reliability बढ़ती है। / Provided buffer sets pH salt and cofactors according to the enzyme. This increases digestion reliability.

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Question 209/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Double digest में enzymes अगर अलग temperatures पर काम करें तो क्या कर सकते हैं?

If enzymes in double digest work at different temperatures what can be done?

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A. sequential digestion plan करेंPlan sequential digestion

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Sequential digestion में enzymes को अलग steps में suitable conditions मिलती हैं। इससे digestion success बढ़ती है। / Sequential digestion gives enzymes suitable conditions in separate steps. This improves digestion success.

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Question 210/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 49

Double digest में one-pot reaction का अर्थ क्या है?

What does one-pot reaction mean in double digest?

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A. दो enzymes एक ही tube में साथ use करनाUsing two enzymes together in one tube

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One-pot double digestion तभी अच्छा है जब दोनों enzymes compatible buffer और temperature में काम करें। / One-pot double digestion is good only when both enzymes work in compatible buffer and temperature.

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