A. दो enzymes एक ही tube में साथ use करना/Using two enzymes together in one tube
Step 1
Concept
One-pot double digestion is good only when both enzymes work in compatible buffer and temperature.
Step 2
Why this answer is correct
The correct answer is A. दो enzymes एक ही tube में साथ use करना / Using two enzymes together in one tube. One-pot double digestion is good only when both enzymes work in compatible buffer and temperature.
Step 3
Exam Tip
One-pot double digestion तभी अच्छा है जब दोनों enzymes compatible buffer और temperature में काम करें।
A. जब दो enzymes एक buffer में अच्छे से काम न करें/When two enzymes do not work well in one buffer
Step 1
Concept
In sequential digestion enzymes are used in separate steps. It helps when buffers are incompatible.
Step 2
Why this answer is correct
The correct answer is A. जब दो enzymes एक buffer में अच्छे से काम न करें / When two enzymes do not work well in one buffer. In sequential digestion enzymes are used in separate steps. It helps when buffers are incompatible.
Step 3
Exam Tip
Sequential digestion में enzymes अलग-अलग steps में use किए जाते हैं। यह buffer incompatibility में मदद करता है।
D. दोनों enzymes की activity बनाए रखने के लिए/To maintain activity of both enzymes
Step 1
Concept
Two enzymes can show different efficiencies in the same buffer. Compatible buffer is necessary for digestion success.
Step 2
Why this answer is correct
The correct answer is D. दोनों enzymes की activity बनाए रखने के लिए / To maintain activity of both enzymes. Two enzymes can show different efficiencies in the same buffer. Compatible buffer is necessary for digestion success.
Step 3
Exam Tip
दो enzymes एक ही buffer में अलग-अलग efficiency दिखा सकते हैं। Compatible buffer digestion success के लिए जरूरी है।
B. एक DNA sample को दो restriction enzymes से काटना/Cutting one DNA sample with two restriction enzymes
Step 1
Concept
In double digestion two different restriction enzymes act on one DNA sample. It can be useful in directional cloning.
Step 2
Why this answer is correct
The correct answer is B. एक DNA sample को दो restriction enzymes से काटना / Cutting one DNA sample with two restriction enzymes. In double digestion two different restriction enzymes act on one DNA sample. It can be useful in directional cloning.
Step 3
Exam Tip
Double digestion में दो अलग restriction enzymes एक DNA sample पर काम करते हैं। यह directional cloning में उपयोगी हो सकता है।
A. दिशात्मक क्लोनिंग आसान हो सकती है/Directional cloning can become easier
Step 1
Concept
Two different ends make correct orientation of an insert easier. In exams link directional cloning with two enzymes.
Step 2
Why this answer is correct
The correct answer is A. दिशात्मक क्लोनिंग आसान हो सकती है / Directional cloning can become easier. Two different ends make correct orientation of an insert easier. In exams link directional cloning with two enzymes.
Step 3
Exam Tip
दो अलग सिरों से खंड को सही दिशा में लगाना आसान होता है। परीक्षा में directional cloning को दो एंजाइमों से जोड़ें।
A. एक ही डीएनए को दो प्रतिबंध एंजाइमों से काटना/Cutting the same DNA with two restriction enzymes
Step 1
Concept
In double digestion two enzymes act on the same sample. This allows use of two different cut sites.
Step 2
Why this answer is correct
The correct answer is A. एक ही डीएनए को दो प्रतिबंध एंजाइमों से काटना / Cutting the same DNA with two restriction enzymes. In double digestion two enzymes act on the same sample. This allows use of two different cut sites.
Step 3
Exam Tip
डबल डाइजेशन में दो एंजाइम एक ही नमूने पर काम करते हैं। इससे दो अलग कट स्थल उपयोग किए जा सकते हैं।
