Restriction digestion के बाद enzyme activity बची रहे तो next cloning step में क्या risk है?
If enzyme activity remains after restriction digestion what is the risk in next cloning step?
Explanation opens after your attempt
A. unwanted further cutting हो सकती हैUnwanted further cutting can occur
Concept
Active enzyme can cut DNA ends again in ligation mix. Cleanup or inactivation can be useful.
Why this answer is correct
The correct answer is A. unwanted further cutting हो सकती है / Unwanted further cutting can occur. Active enzyme can cut DNA ends again in ligation mix. Cleanup or inactivation can be useful.
Exam Tip
Active enzyme ligation mix में DNA ends को फिर काट सकता है। Cleanup या inactivation useful हो सकती है।
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