100 results found for "plasmid cloning vector" in all classes.
Which vector example is linked with bacterial plasmid cloning?
A. pBR322
pBR322 plasmid cloning vector का classic example है। इसे bacterial host में use किया जा सकता है। / pBR322 is a classic example of a plasmid cloning vector. It can be used in bacterial hosts.
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What is a simple difference between expression vector and cloning vector?
A. Expression vector gene product बनवाने में मदद करता हैExpression vector helps produce gene product
Cloning vector DNA की copies बनाने के लिए होता है। Expression vector gene expression और product formation में मदद करता है। / A cloning vector is for making DNA copies. An expression vector helps gene expression and product formation.
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Which vector can be more suitable than a plasmid for large insert cloning?
A. BAC
BAC large DNA insert cloning के लिए उपयोगी हो सकता है। Simple plasmids अक्सर छोटे inserts के लिए ज्यादा convenient होते हैं। / BAC can be useful for large DNA insert cloning. Simple plasmids are often more convenient for smaller inserts.
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Which task becomes difficult in gene cloning without a cloning vector?
A. Desired DNA को host में carry करनाCarrying desired DNA into host
Vector desired DNA को host में carry करता है। इसके बिना desired DNA copies बनवाना कठिन होगा। / A vector carries desired DNA into a host. Without it making copies of desired DNA becomes difficult.
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What is one simple difference between a cloning vector and an expression vector?
A. क्लोनिंग vector copy बनाता है और expression vector product बनवाता हैCloning vector makes copies and expression vector helps make product
Cloning vector का लक्ष्य DNA copies बढ़ाना है। Expression vector gene product बनवाने के लिए design हो सकता है। / The aim of a cloning vector is to increase DNA copies. An expression vector can be designed to produce gene product.
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Which statement is correct about plasmid cloning vectors?
A. वे छोटे circular DNA carriers हो सकते हैंThey can be small circular DNA carriers
Plasmid vectors circular DNA carriers की तरह काम करते हैं। वे host में replicate हो सकते हैं। / Plasmid vectors work like circular DNA carriers. They can replicate in a host.
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What would be difficult in gene cloning without a cloning vector?
A. Desired DNA को host में ले जाकर copy बनानाCarrying desired DNA into host and making copies
Vector desired DNA को host में पहुँचाकर replication में मदद करता है। इसलिए gene cloning में vector जरूरी है। / A vector carries desired DNA into a host and helps replication. Therefore a vector is necessary in gene cloning.
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Which host is commonly used in plasmid cloning?
A. एशेरिशिया कोलाईEscherichia coli
Escherichia coli plasmid cloning का common host है। यह तेजी से बढ़ता है और lab में संभालना आसान है। / Escherichia coli is a common host for plasmid cloning. It grows fast and is easy to handle in labs.
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Which molecule is commonly studied as a cloning vector?
A. प्लास्मिडPlasmid
प्लास्मिड छोटे वृत्ताकार डीएनए अणु होते हैं और cloning vectors के रूप में बहुत उपयोगी हैं। परीक्षा में plasmid को common vector याद रखें। / Plasmids are small circular DNA molecules and are very useful as cloning vectors. Remember plasmid as a common vector.
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Which statement about plasmid vector is wrong?
D. यह हमेशा chlorophyll pigment होता हैIt is always chlorophyll pigment
Plasmid vector DNA carrier होता है pigment नहीं। इसमें ori और selectable marker जैसे parts हो सकते हैं। / A plasmid vector is a DNA carrier not a pigment. It can have parts like ori and selectable marker.
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What becomes difficult if a cloning vector has no cloning site?
A. इंसर्ट डीएनए को जोड़नाInserting DNA
Cloning site insert DNA जोड़ने का designed स्थान देती है। इसके बिना insertion नियंत्रित नहीं रहेगा। / A cloning site provides the designed location for adding insert DNA. Without it insertion will not be controlled.
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What is the best easy-level summary of a cloning vector?
A. Ori marker और cloning site वाला DNA carrierDNA carrier with ori marker and cloning site
Easy level पर cloning vector को DNA carrier मानें जिसमें ori marker और cloning site होते हैं। इन्हीं functions से questions बनते हैं। / At easy level treat cloning vector as a DNA carrier with ori marker and cloning site. Questions are built from these functions.
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Which statement about a cloning vector is correct?
A. यह DNA carrier की तरह काम करता हैIt acts as a DNA carrier
Cloning vector desired DNA को host में carry करता है। यह gene cloning का basic tool है। / A cloning vector carries desired DNA into a host. It is a basic tool of gene cloning.
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In which case can a simple cloning vector be sufficient?
A. जब केवल DNA copies चाहिएWhen only DNA copies are needed
Simple cloning vector DNA की copies बनाने के लिए useful है। Gene product चाहिए तो expression vector बेहतर हो सकता है। / A simple cloning vector is useful for making DNA copies. If gene product is needed an expression vector may be better.
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Why can supercoiled plasmid look different from linear plasmid on gel?
A. supercoiled shape migration बदलती हैSupercoiled shape changes migration
Plasmid conformation gel mobility को प्रभावित करती है। इसलिए uncut plasmid के bands अलग दिख सकते हैं। / Plasmid conformation affects gel mobility. Therefore uncut plasmid bands can look different.
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Which cloning vector is useful with a common bacterial host?
A. Plasmid vector
Plasmid vectors अक्सर E coli जैसे bacterial host में उपयोग किए जाते हैं। ये simple cloning में common हैं। / Plasmid vectors are often used in bacterial hosts like E coli. They are common in simple cloning.
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Which is a correct example of a cloning vector?
A. पीबीआर तीन सौ बाईसpBR322
pBR322 एक प्रसिद्ध plasmid cloning vector है। इसमें origin और selectable markers जैसे भाग होते हैं। / pBR322 is a famous plasmid cloning vector. It has parts such as origin and selectable markers.
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Which option gives the correct basic structure of a plasmid vector?
A. छोटा circular double-stranded DNASmall circular double-stranded DNA
Plasmid vectors सामान्यतः छोटे circular double-stranded DNA होते हैं। ये host में replicate कर सकते हैं। / Plasmid vectors are usually small circular double-stranded DNA. They can replicate in a host.
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What is the use of a multiple cloning site in a cloning vector?
A. कई restriction sites देनाProviding many restriction sites
Multiple cloning site में कई restriction enzyme sites होते हैं। इससे insert जोड़ने के विकल्प बढ़ जाते हैं। / A multiple cloning site contains many restriction enzyme sites. It increases options for inserting DNA.
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Which option can improve cloning efficiency of a vector?
A. Clear selectable marker और useful cloning siteClear selectable marker and useful cloning site
Clear marker selection में और useful cloning site insertion में मदद करती है। ये vector efficiency बढ़ा सकते हैं। / A clear marker helps selection and a useful cloning site helps insertion. These can increase vector efficiency.
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Which is the safest short note on cloning vector?
A. यह foreign DNA को host में carry और replicate कराने वाला DNA vehicle हैIt is a DNA vehicle that carries and replicates foreign DNA in a host
Cloning vector foreign DNA को host में पहुँचाकर उसकी copies बनवाता है। इसमें ori marker और cloning site जैसे parts होते हैं। / A cloning vector carries foreign DNA into a host and helps make copies. It has parts like ori marker and cloning site.
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Which option is a wrong application of cloning vector?
D. Starch digestion enzyme के रूप में उपयोगUse as starch-digesting enzyme
Cloning vectors DNA carriers हैं। वे starch digestion enzymes नहीं होते। / Cloning vectors are DNA carriers. They are not starch-digesting enzymes.
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Which vector part is often checked first on a map for cloning planning?
A. MCS restriction sites
MCS बताता है कि कौन से restriction sites insert cloning के लिए उपलब्ध हैं। यह cloning strategy तय करने में मदद करता है। / MCS shows which restriction sites are available for insert cloning. It helps decide the cloning strategy.
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Which option is the exam-level identity of a cloning vector?
A. Ori marker और cloning site वाला DNA vehicleDNA vehicle with ori marker and cloning site
Exam में cloning vector को DNA vehicle मानें जिसमें replication selection और insertion features हों। यही safest answer है। / In exams treat a cloning vector as a DNA vehicle with replication selection and insertion features. This is the safest answer.
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Which statement is correct for a cloning vector?
A. It must be able to carry foreign DNA
Cloning vector का basic function foreign DNA carry करना है। यही gene cloning का first requirement है। / The basic function of a cloning vector is carrying foreign DNA. This is the first requirement of gene cloning.
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In a cDNA library what can a cloning vector carry?
A. mRNA से बने complementary DNA fragmentsComplementary DNA fragments made from mRNA
cDNA library में expressed genes से बने complementary DNA fragments clone किए जाते हैं। Vector इन fragments को carry करता है। / In a cDNA library complementary DNA fragments made from expressed genes are cloned. The vector carries these fragments.
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How can directional cloning reduce empty vector problem?
A. दो अलग ends से self-joining कठिन हो सकती हैTwo different ends can make self-joining difficult
दो अलग restriction ends vector के self-ligation को घटा सकते हैं। इससे desired insert cloning बेहतर हो सकती है। / Two different restriction ends can reduce vector self-ligation. This can improve desired insert cloning.
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Why is a self-ligated vector a problem in cloning?
A. Vector insert के बिना बंद हो जाता हैVector closes without insert
Self-ligation में vector अपने ही सिरों से जुड़ जाता है। इससे desired recombinant clone नहीं बनता। / In self-ligation the vector joins its own ends. This does not form the desired recombinant clone.
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Which option is the safest definition of a cloning vector?
A. विदेशी डीएनए को host में ले जाने वाला डीएनए वाहकA DNA carrier that takes foreign DNA into a host
Cloning vector foreign DNA को host में carry करता है। यह DNA cloning का basic vehicle है। / A cloning vector carries foreign DNA into a host. It is the basic vehicle of DNA cloning.
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Which statement about a cloning vector is incorrect?
D. यह हमेशा starch digestion enzyme होता हैIt is always a starch-digesting enzyme
Cloning vector DNA carrier होता है enzyme नहीं। इसका काम foreign DNA को host में ले जाना है। / A cloning vector is a DNA carrier not a digestive enzyme. Its role is to carry foreign DNA into a host.
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Which option correctly relates cloning vector and insert size?
A. Different vectors different insert sizes carry कर सकते हैंDifferent vectors can carry different insert sizes
Plasmid phage BAC और YAC जैसे vectors की insert capacity अलग हो सकती है। Vector choice insert size पर निर्भर हो सकती है। / Vectors such as plasmid phage BAC and YAC can have different insert capacities. Vector choice can depend on insert size.
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Which option is most correct for a cloning vector?
A. It is a DNA vehicle for cloning a desired DNA segment
Cloning vector desired DNA segment को host में carry करता है। यह DNA cloning का vehicle है। / A cloning vector carries a desired DNA segment into a host. It is the vehicle of DNA cloning.
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What does directional cloning vector design help with?
A. Insert orientation control
Directional cloning अलग ends के कारण insert को desired direction में जोड़ने में मदद करती है। यह expression experiments में उपयोगी हो सकता है। / Directional cloning uses different ends to help insert DNA in the desired direction. It can be useful in expression experiments.
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Which feature can make a vector unsafe or less useful for cloning?
A. Selectable marker की कमीLack of selectable marker
Selectable marker न हो तो transformed cells चुनना कठिन होगा। इसलिए marker की कमी vector usefulness घटाती है। / Without a selectable marker it is difficult to select transformed cells. Therefore lack of marker reduces vector usefulness.
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Why can insert orientation matter in a cloning vector?
A. Expression या downstream use प्रभावित हो सकता हैExpression or downstream use can be affected
Insert की दिशा gene expression या analysis को प्रभावित कर सकती है। Directional cloning orientation control में मदद करती है। / Insert direction can affect gene expression or analysis. Directional cloning helps control orientation.
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What is the safest definition of a cloning vector?
A. वह डीएनए वाहक जो foreign DNA को host में ले जाकर replicate कराता हैA DNA carrier that takes foreign DNA into host and helps it replicate
Cloning vector foreign DNA को host में carry करता है और copy number बढ़ाने में मदद करता है। यही topic का मूल सार है। / A cloning vector carries foreign DNA into a host and helps increase copy number. This is the core summary of the topic.
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Which option is a correct group of features of a cloning vector?
A. ओरी selectable marker और cloning siteOri selectable marker and cloning site
एक अच्छे cloning vector में ori marker और cloning site जैसे भाग होने चाहिए। ये replication selection और insertion में मदद करते हैं। / A good cloning vector should have parts like ori marker and cloning site. They help in replication selection and insertion.
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Which cloning vector is known for carrying large DNA inserts?
A. बीएसीBAC
BAC large DNA fragments को clone करने के लिए उपयोगी होता है। Easy level में इसे large insert vector याद रखें। / BAC is useful for cloning large DNA fragments. At easy level remember it as a large insert vector.
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Which option is not an essential part of a cloning vector?
D. क्लोरोफिल pigmentChlorophyll pigment
Cloning vector में ori selectable marker और cloning site जरूरी होते हैं। Chlorophyll pigment cloning vector का आवश्यक भाग नहीं है। / A cloning vector needs ori selectable marker and cloning site. Chlorophyll pigment is not an essential part of a cloning vector.
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When is a promoter more important in a cloning vector?
A. जब insert gene को express कराना होWhen the insert gene needs to be expressed
Expression vectors में promoter gene expression के लिए जरूरी होता है। केवल cloning vector में मुख्य focus DNA copy बनाना होता है। / In expression vectors promoter is needed for gene expression. In simple cloning vectors the main focus is making DNA copies.
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Which option distinguishes an empty vector from a recombinant vector?
A. Recombinant vector में insert होता हैRecombinant vector has insert
Recombinant vector में desired insert DNA जुड़ा होता है। Empty vector में desired insert नहीं होता। / A recombinant vector has the desired insert DNA joined. An empty vector lacks the desired insert.
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Which option distinguishes recombinant vector from empty vector?
A. Recombinant vector में insert DNA होता हैRecombinant vector has insert DNA
Recombinant vector में foreign insert जुड़ा होता है। Empty vector में desired insert नहीं होता। / A recombinant vector has a foreign insert joined. An empty vector lacks the desired insert.
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Why is a multiple cloning site useful in a plasmid?
A. उसमें कई restriction sites होते हैंIt contains many restriction sites
Multiple cloning site में कई restriction sites होने से insert डालने के विकल्प मिलते हैं। इसे cloning flexibility से जोड़ें। / A multiple cloning site provides options for inserting DNA through many restriction sites. Link it with cloning flexibility.
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Which vector can be considered different from plasmid due to large insert capacity?
A. BAC
BAC large inserts को carry करने में useful है। Simple plasmids आमतौर पर smaller inserts के लिए सुविधाजनक होते हैं। / BAC is useful for carrying large inserts. Simple plasmids are usually convenient for smaller inserts.
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Which option correctly links cloning vector and gene library?
A. कई DNA fragments को vectors में store करनाStoring many DNA fragments in vectors
Gene library में अनेक DNA fragments अलग-अलग vectors में cloned रखे जा सकते हैं। यह genome study में उपयोगी है। / In a gene library many DNA fragments can be cloned in different vectors. This is useful in genome study.
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Which option is the correct group of core features of a cloning vector?
A. Ori selectable marker और cloning siteOri selectable marker and cloning site
Ori replication के लिए marker selection के लिए और cloning site insertion के लिए होती है। ये vector के basic parts हैं। / Ori is for replication marker is for selection and cloning site is for insertion. These are basic parts of a vector.
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What is the safest exam answer for a cloning vector?
A. ओरी selectable marker और cloning site वाला डीएनए वाहकDNA carrier with ori selectable marker and cloning site
Exam में cloning vector को DNA carrier मानें जिसमें replication selection और insertion features हों। यही concise answer है। / In exams treat a cloning vector as a DNA carrier with replication selection and insertion features. This is the concise answer.
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Which option is a wrong example of a cloning vector?
D. Pepsin
Pepsin protein digestion enzyme है cloning vector नहीं। pBR322 pUC और Ti plasmid vector examples हैं। / Pepsin is a protein-digesting enzyme not a cloning vector. pBR322 pUC and Ti plasmid are vector examples.
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Why are two different ends made in a vector during directional cloning?
A. Insert को सही दिशा में जोड़ने के लिएTo join insert in correct orientation
दो अलग restriction enzymes अलग ends बना सकते हैं। इससे insert की direction control हो सकती है। / Two different restriction enzymes can create different ends. This can control insert direction.
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Which option is related to correct position of insert in a cloning vector?
A. Cloning site
Insert DNA cloning site या MCS में जोड़ा जाता है। यह vector का designed insertion region होता है। / Insert DNA is added at the cloning site or MCS. This is the designed insertion region of the vector.
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Why is vector copy number important in cloning?
A. यह DNA yield को प्रभावित कर सकता हैIt can affect DNA yield
Higher copy number से अधिक plasmid DNA मिल सकता है। इसलिए copy number cloning output से जुड़ा है। / Higher copy number can provide more plasmid DNA. Therefore copy number is linked with cloning output.
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What problem can occur if a cloning vector has too many random restriction sites?
A. अनचाहे कट और cloning difficultyUnwanted cuts and cloning difficulty
Controlled cloning के लिए useful और defined restriction sites चाहिए। Random multiple cuts vector को नुकसान पहुँचा सकते हैं। / Controlled cloning needs useful and defined restriction sites. Random multiple cuts can damage the vector.
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Which issue can affect insert stability in a cloning vector?
A. Host में insert का unstable होनाInsert being unstable in host
कुछ inserts host में unstable हो सकते हैं। Vector-host system का चुनाव stability पर असर डाल सकता है। / Some inserts can be unstable in a host. Choice of vector-host system can affect stability.
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Which cloning result most clearly identifies a recombinant vector?
A. Vector में desired insert confirm होDesired insert is confirmed in vector
Recombinant vector में desired insert मौजूद होना चाहिए। Confirmation restriction analysis या sequencing से की जा सकती है। / A recombinant vector must contain the desired insert. Confirmation can be done by restriction analysis or sequencing.
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In which condition will cloning vector maintenance be better?
A. Functional ori और selection pressure होFunctional ori and selection pressure are present
Functional ori vector replication कराता है और selection pressure marker वाला vector बनाए रखने में मदद करता है। / Functional ori allows vector replication and selection pressure helps maintain marker-containing vector.
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Which option is a correct goal of cloning vector design?
A. Replication selection और insertion को आसान बनानाMaking replication selection and insertion easy
Vector design में ori marker और cloning site जैसे elements रखे जाते हैं। ये cloning को efficient बनाते हैं। / Vector design includes elements like ori marker and cloning site. These make cloning efficient.
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What can be the main next lab step after insert cloning into a vector?
A. Transformation into host cell
Recombinant vector बनने के बाद उसे host cell में डाला जा सकता है। इसे transformation कहा जाता है। / After a recombinant vector is made it can be introduced into a host cell. This is called transformation.
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Which feature makes a cloning vector useful for selection?
A. सेलेक्टेबल मार्करSelectable marker
Selectable marker transformed cells को चुनने में मदद करता है। यह vector design का प्रमुख भाग है। / A selectable marker helps select transformed cells. It is a key part of vector design.
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How do copies of desired DNA increase through a cloning vector?
A. Vector host में replicate होता हैVector replicates in the host
Insert vector के साथ host में replicate होता है। इस तरह desired DNA की copies बढ़ती हैं। / The insert replicates with the vector in the host. In this way copies of desired DNA increase.
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Why is empty vector problem unwanted in cloning?
A. Host cells vector तो लेती हैं पर desired insert नहीं होताHost cells take vector but desired insert is absent
Empty vector colonies selection में बच सकती हैं लेकिन desired gene नहीं रखतीं। इसलिए screening जरूरी है। / Empty vector colonies may survive selection but lack the desired gene. Therefore screening is needed.
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Why can a promoter be added in a cloning vector?
A. Gene expression कराने के लिएTo enable gene expression
Promoter gene transcription शुरू कराने में मदद करता है। यह expression vector में अधिक महत्वपूर्ण होता है। / A promoter helps start gene transcription. It is more important in an expression vector.
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After adding the desired gene into a cloning vector in which cell is it grown?
A. Host cell
Recombinant vector को host cell में introduce किया जाता है। Host cell vector और insert की copies बना सकती है। / A recombinant vector is introduced into a host cell. The host cell can make copies of the vector and insert.
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What is the advantage of small size in a cloning vector?
A. Manipulation और transformation आसान हो सकते हैंManipulation and transformation can be easier
छोटे vectors को isolate और introduce करना आसान होता है। इसलिए small size cloning में practical advantage है। / Small vectors are easier to isolate and introduce. Therefore small size is a practical advantage in cloning.
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Which enzyme performs joining when a foreign gene is added to a cloning vector?
A. डीएनए लाइगेजDNA ligase
DNA ligase foreign gene और vector ends को जोड़ता है। Restriction enzymes पहले compatible ends बना सकते हैं। / DNA ligase joins the foreign gene and vector ends. Restriction enzymes can create compatible ends first.
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Which feature helps maintain a cloning vector in a host?
A. ओरी और selectable markerOri and selectable marker
ओरी प्रतिकृति कराता है और marker selection में मदद करता है। दोनों vector maintenance के लिए महत्वपूर्ण हैं। / Ori allows replication and marker helps in selection. Both are important for vector maintenance.
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What size of cloning vector is generally considered more convenient?
B. छोटा और आसानी से संभलने वालाSmall and easy to handle
छोटे वेक्टर को अलग करना और होस्ट में पहुँचाना आसान होता है। परीक्षा में small size को useful feature मानें। / A small vector is easier to isolate and introduce into a host. In exams treat small size as a useful feature.
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For a very large insert which vector can be considered instead of a simple plasmid?
A. BAC या YACBAC or YAC
BAC और YAC बड़े DNA inserts के लिए उपयोगी हो सकते हैं। Simple plasmid अक्सर छोटे inserts के लिए convenient है। / BAC and YAC can be useful for large DNA inserts. A simple plasmid is often convenient for smaller inserts.
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A plasmid vector has ampR gene. What is its use on an ampicillin plate?
A. ampicillin-resistant transformed cells चुननाSelecting ampicillin-resistant transformed cells
ampR gene ampicillin resistance देता है। इससे vector-containing cells ampicillin plate पर grow कर सकती हैं। / ampR gene gives ampicillin resistance. Thus vector-containing cells can grow on an ampicillin plate.
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A plasmid vector has ori but no marker. What will be the main difficulty?
A. Transformed cells को चुनना कठिन होगाSelecting transformed cells will be difficult
Ori replication में मदद करता है लेकिन marker selection के लिए चाहिए। Marker न होने पर transformed cells पहचानना कठिन है। / Ori helps in replication but marker is needed for selection. Without marker identifying transformed cells is difficult.
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Why is a plasmid considered a good cloning vector?
A. इसमें प्रतिकृति क्षमता और विदेशी डीएनए ले जाने की क्षमता हो सकती हैIt can replicate and carry foreign DNA
अच्छे प्लाज्मिड वाहक में प्रतिकृति उद्गम और चयन चिह्न जैसे गुण हो सकते हैं। इसलिए यह जीन क्लोनिंग में उपयोगी है। / A good plasmid vector may have an origin of replication and selectable marker. That makes it useful in gene cloning.
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What does host range mean for a cloning vector?
A. वेक्टर किन hosts में काम कर सकता हैIn which hosts the vector can work
Host range vector की compatibility बताता है। Broad host range vector कई hosts में काम कर सकता है। / Host range indicates vector compatibility. A broad host range vector can work in many hosts.
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Which vector feature can reduce vector loss during repeated subculture?
A. Selectable marker with selection
Selection marker और selection condition vector-containing cells को बनाए रखने में मदद करते हैं। इससे vector loss घट सकता है। / A selection marker and selection condition help maintain vector-containing cells. This can reduce vector loss.
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What can happen to cloning if plasmid prep is not clean in restriction digestion?
A. digestion और ligation दोनों प्रभावित हो सकते हैंBoth digestion and ligation can be affected
Contaminants restriction enzyme और ligase reactions को inhibit कर सकते हैं। Clean preparation important है। / Contaminants can inhibit restriction enzyme and ligase reactions. Clean preparation is important.
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On what basis can a plasmid and BAC be chosen?
A. Insert size और experiment needInsert size and experiment need
Small inserts के लिए plasmid convenient हो सकता है और large inserts के लिए BAC useful हो सकता है। Vector choice insert size पर निर्भर हो सकती है। / A plasmid can be convenient for small inserts and BAC can be useful for large inserts. Vector choice can depend on insert size.
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Ti plasmid is associated with which bacterium?
A. Agrobacterium tumefaciens
Ti plasmid Agrobacterium tumefaciens में पाया जाता है। Modified Ti plasmid plant transformation में उपयोगी है। / Ti plasmid is found in Agrobacterium tumefaciens. Modified Ti plasmid is useful in plant transformation.
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What must be joined to form a recombinant plasmid in cloning?
A. प्लास्मिड वेक्टर और insert डीएनएPlasmid vector and insert DNA
Recombinant plasmid में vector और insert DNA का जुड़ना जरूरी है। यह जोड़ DNA ligase करता है। / A recombinant plasmid needs joining of vector and insert DNA. DNA ligase performs this joining.
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When is high copy number useful in a cloning vector?
A. जब अधिक plasmid DNA चाहिएWhen more plasmid DNA is needed
High copy number से प्रति cell अधिक plasmid copies मिल सकती हैं। इससे plasmid DNA yield बढ़ती है। / High copy number can give more plasmid copies per cell. This increases plasmid DNA yield.
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What type of vector is pBR322?
A. Plasmid cloning vector
pBR322 एक प्रसिद्ध plasmid cloning vector है। इसे class 11 biotechnology में common example माना जाता है। / pBR322 is a famous plasmid cloning vector. It is considered a common example in Class 11 biotechnology.
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Which option gives the correct identity of a plasmid?
B. छोटा स्वतंत्र वृत्ताकार डीएनएSmall independent circular DNA
प्लास्मिड छोटे स्वतंत्र वृत्ताकार डीएनए अणु होते हैं। वे क्लोनिंग वेक्टर के रूप में बहुत उपयोगी हैं। / Plasmids are small independent circular DNA molecules. They are very useful as cloning vectors.
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If foreign DNA is successfully joined into a plasmid, what can the plasmid be called?
A. पुनः संयोजित प्लाज्मिडRecombinant plasmid
विदेशी डीएनए जुड़ने के बाद प्लाज्मिड पुनः संयोजित प्लाज्मिड कहलाता है। यह मेजबान में डाला जा सकता है। / After foreign DNA is inserted, the plasmid is called a recombinant plasmid. It can be introduced into a host.
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Which option gives three core functions of cloning vector correctly?
A. Carry insert replicate in host allow selection
Cloning vector insert carry करता है host में replicate होता है और selection में मदद करता है। ये easy exam functions हैं। / A cloning vector carries insert replicates in host and helps selection. These are easy exam functions.
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Which option gives the correct role of antibiotic in cloning vector selection?
A. Marker वाले cells को चुनने में मददHelps select cells with marker
Antibiotic केवल उन cells को बढ़ने देता है जिनमें resistance marker होता है। इससे selection संभव होती है। / Antibiotic allows only cells with resistance marker to grow. This enables selection.
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Which option gives a correct application of a cloning vector?
A. Desired gene की copies बनानाMaking copies of a desired gene
Cloning vector desired gene को host में replicate करवा सकता है। यही gene cloning का main goal है। / A cloning vector can replicate a desired gene in a host. This is the main goal of gene cloning.
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What can sequencing most clearly tell in a cloning vector?
A. Insert का exact sequence और directionExact sequence and direction of insert
Sequencing insert की exact sequence और orientation बता सकती है। यह clone confirmation का strong तरीका है। / Sequencing can tell the exact sequence and orientation of the insert. It is a strong method for clone confirmation.
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What can be confirmed in a cloning vector by sequencing?
A. Insert sequence और orientationInsert sequence and orientation
Sequencing insert का सही sequence और direction confirm कर सकती है। यह final confirmation में उपयोगी है। / Sequencing can confirm the correct sequence and direction of the insert. It is useful for final confirmation.
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Why can insert orientation be important in a cloning vector?
A. Expression या आगे के प्रयोग प्रभावित हो सकते हैंExpression or downstream experiments can be affected
Insert की दिशा gene expression या analysis को प्रभावित कर सकती है। Directional cloning orientation control में मदद करती है। / Insert direction can affect gene expression or analysis. Directional cloning helps control orientation.
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What forms after foreign DNA is joined into a cloning vector?
A. रिकॉम्बिनेंट वेक्टरRecombinant vector
विदेशी डीएनए जुड़ने पर vector recombinant vector बन जाता है। इसे host में बढ़ाया जा सकता है। / When foreign DNA is joined the vector becomes a recombinant vector. It can be propagated in a host.
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Why is small size beneficial in a good cloning vector?
A. क्योंकि छोटा वेक्टर संभालना और स्थानांतरित करना आसान होता हैBecause a small vector is easier to handle and transfer
छोटे वेक्टर को isolate करना और होस्ट में डालना आसान होता है। इसलिए small size practical advantage है। / A small vector is easier to isolate and introduce into a host. Therefore small size is a practical advantage.
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What is the most direct purpose of inserting foreign DNA into a cloning vector?
A. विदेशी डीएनए की प्रतियाँ बनवानाMaking copies of foreign DNA
क्लोनिंग वेक्टर विदेशी डीएनए को होस्ट में ले जाकर उसकी प्रतियाँ बनवाता है। परीक्षा में इसे डीएनए कॉपी बनाने वाला वाहक याद रखें। / A cloning vector carries foreign DNA into a host and helps make copies. Remember it as a DNA-copy carrier for exams.
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Which option is a wrong use of selectable marker in cloning vector?
A. DNA fragment को directly cut करनाDirectly cutting DNA fragment
Selectable marker DNA को cut नहीं करता। DNA cutting restriction enzyme का काम है। / A selectable marker does not cut DNA. DNA cutting is the job of restriction enzymes.
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What can sequencing tell about an insert in a cloning vector?
A. Insert sequence और orientationInsert sequence and orientation
Sequencing insert की correct sequence और orientation confirm कर सकती है। यह high-confidence confirmation method है। / Sequencing can confirm correct insert sequence and orientation. It is a high-confidence confirmation method.
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Which option states the core purpose of a cloning vector?
A. Foreign DNA को carry और multiply करनाTo carry and multiply foreign DNA
Cloning vector foreign DNA को host में carry करता है और उसकी copies बनवाता है। यही इसका core purpose है। / A cloning vector carries foreign DNA into a host and allows copies to be made. This is its core purpose.
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How can the presence of insert DNA in a cloning vector be confirmed?
A. Screening methods सेBy screening methods
Screening methods recombinants की पहचान में मदद करती हैं। Blue-white screening या restriction analysis examples हो सकते हैं। / Screening methods help identify recombinants. Blue-white screening or restriction analysis can be examples.
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Why are competent cells made before introducing a cloning vector into a host?
A. DNA uptake आसान करने के लिएTo make DNA uptake easier
Competent cells बाहरी DNA लेने के लिए तैयार होती हैं। Transformation में यह step उपयोगी है। / Competent cells are prepared to take up external DNA. This step is useful in transformation.
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On which medium can a selectable marker of cloning vector be useful?
A. Antibiotic-containing medium
Antibiotic resistance marker antibiotic-containing medium पर transformed cells select करने में मदद करता है। यह selection का direct example है। / An antibiotic resistance marker helps select transformed cells on antibiotic-containing medium. This is a direct example of selection.
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The idea of ori in a cloning vector is related to what?
A. प्रतिकृति का आरंभStart of replication
ओरी वह स्थान है जहाँ से vector replication शुरू हो सकती है। परीक्षा में ori को replication start point याद रखें। / Ori is the site from which vector replication can start. In exams remember ori as the replication start point.
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What helps identify transformation success in a cloning vector?
A. Selectable marker
Selectable marker बताता है कि कौन सी कोशिकाएँ vector लेकर बढ़ रही हैं। यह transformed cells पहचानने में मदद करता है। / A selectable marker shows which cells are growing with the vector. It helps identify transformed cells.
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What is the purpose of inserting foreign DNA into a cloning vector?
A. उसकी कई प्रतियाँ बनवानाTo make many copies of it
Vector foreign DNA को host में ले जाता है जहाँ उसकी प्रतियाँ बन सकती हैं। यही cloning का मुख्य उद्देश्य है। / The vector carries foreign DNA into a host where copies can be made. This is the main purpose of cloning.
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Ampicillin resistance gene in a cloning vector is an example of what?
A. Selectable marker
Ampicillin resistance gene selection में उपयोगी marker है। यह transformed cells की पहचान में मदद करता है। / Ampicillin resistance gene is a useful marker for selection. It helps identify transformed cells.
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What is formed after insert DNA is joined into a cloning vector?
A. रिकॉम्बिनेंट वेक्टरRecombinant vector
Insert DNA जुड़ने पर vector recombinant vector बन जाता है। यह cloning experiment का मुख्य product है। / When insert DNA is added the vector becomes a recombinant vector. This is the main product of a cloning experiment.
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