Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 211-220 of 290 questions.
Which molecule provides energy for T4 DNA ligase?
B. ATP
T4 DNA ligase ATP dependent है। यह ATP की ऊर्जा से DNA ends जोड़ता है। / T4 DNA ligase is ATP dependent. It joins DNA ends using energy from ATP.
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Which cofactor should be remembered for E coli DNA ligase?
C. NAD plus
E coli DNA ligase सामान्यतः NAD plus dependent माना जाता है। यह T4 DNA ligase से अलग direct point है। / E coli DNA ligase is generally considered NAD plus dependent. This is a direct point different from T4 DNA ligase.
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A recombinant plasmid made using ligase consists of what?
A. vector DNA और insert DNA का joined moleculeJoined molecule of vector DNA and insert DNA
Recombinant plasmid में vector DNA और insert DNA जुड़े होते हैं। Ligase इस joining को स्थायी बनाता है। / A recombinant plasmid has vector DNA and insert DNA joined together. Ligase makes this joining permanent.
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Which step may come just after ligation in cloning?
B. transformation into host cell
Ligated recombinant DNA को host cell में introduce किया जा सकता है। यह transformation step हो सकता है। / Ligated recombinant DNA can be introduced into a host cell. This can be the transformation step.
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Why is self-ligation unwanted in cloning?
A. यह vector को insert के बिना बंद कर सकता हैIt can close vector without insert
Self-ligation में vector अपने ही सिरों से जुड़ जाता है। इससे desired insert वाला recombinant plasmid नहीं बनता। / In self-ligation the vector joins its own ends. This does not make the desired recombinant plasmid with insert.
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Directional cloning can improve which result related to ligase?
A. insert को desired orientation में जुड़वानाJoining insert in desired orientation
Directional cloning अलग-अलग ends का उपयोग कर insert orientation को control करती है। Ligase फिर matching ends को जोड़ता है। / Directional cloning uses different ends to control insert orientation. Ligase then joins matching ends.
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Why can vector-to-insert ratio matter in a ligase reaction?
A. सही ratio desired recombinant molecules बढ़ा सकता हैA proper ratio can increase desired recombinant molecules
Vector और insert का उचित अनुपात desired ligation की संभावना बढ़ा सकता है। आसान स्तर पर इसे reaction planning से जोड़ें। / A proper ratio of vector and insert can increase the chance of desired ligation. At easy level link it with reaction planning.
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What problem can occur if insert amount is too low in a ligation reaction?
A. desired recombinant plasmid कम बन सकते हैंFewer desired recombinant plasmids may form
Insert कम होने पर vector के साथ desired joining की संभावना घट सकती है। cloning में insert amount important factor है। / If insert is low the chance of desired joining with vector may decrease. Insert amount is an important factor in cloning.
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Which option is correct for ligase storage practice?
A. cold condition में रखनाKeep in cold condition
Ligase heat sensitive protein enzyme हो सकता है। Cold storage उसकी activity बचाने में मदद करता है। / Ligase can be a heat-sensitive protein enzyme. Cold storage helps preserve its activity.
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Which option correctly gives the substrate of ligase?
A. nicked या cut-ended DNANicked or cut-ended DNA
DNA ligase nicked या cut-ended DNA पर काम करता है। यह DNA backbone में bond बनाता है। / DNA ligase acts on nicked or cut-ended DNA. It forms a bond in the DNA backbone.
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