Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
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Showing 201-210 of 290 questions.
What is the correct difference between ligase and helicase?
B. ligase joins DNA और helicase unwinds DNALigase joins DNA and helicase unwinds DNA
Helicase double helix खोलता है। Ligase DNA fragments को जोड़ता है। / Helicase unwinds the double helix. Ligase joins DNA fragments.
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Ligase mainly acts on which molecule?
C. डीएनएDNA
DNA ligase DNA के nicked या cut ends पर काम करता है। यह food-digesting enzyme नहीं है। / DNA ligase acts on nicked or cut ends of DNA. It is not a food-digesting enzyme.
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Which is the correct chemical pair in a ligase reaction?
A. 5 prime phosphate और 3 prime hydroxyl5 prime phosphate and 3 prime hydroxyl
DNA ligase 5 prime phosphate और 3 prime hydroxyl के बीच phosphodiester bond बनाता है। इसे chemical basis के रूप में याद रखें। / DNA ligase forms a phosphodiester bond between 5 prime phosphate and 3 prime hydroxyl. Remember this as the chemical basis.
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What may happen if 5 prime phosphate is absent in ligation?
A. ligation efficiency घट सकती हैLigation efficiency may decrease
5 prime phosphate phosphodiester bond formation के लिए जरूरी है। इसकी कमी ligation को प्रभावित कर सकती है। / 5 prime phosphate is needed for phosphodiester bond formation. Its absence can affect ligation.
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Which option would not be considered necessary for ligation?
D. photosynthesis pigment
Ligation के लिए active ligase proper DNA ends और suitable buffer जरूरी हैं। Photosynthesis pigment का ligation से संबंध नहीं है। / Ligation needs active ligase proper DNA ends and suitable buffer. Photosynthesis pigment is not related to ligation.
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Why is T4 DNA ligase useful in blunt-end cloning too?
A. यह blunt DNA ends को भी जोड़ सकता हैIt can also join blunt DNA ends
T4 DNA ligase sticky और blunt दोनों ends जोड़ सकता है। Blunt-end ligation में conditions अधिक सावधानी से रखी जाती हैं। / T4 DNA ligase can join both sticky and blunt ends. In blunt-end ligation conditions are kept more carefully.
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Temporary joining of sticky ends occurs by what?
B. base pairing
Sticky ends के complementary bases पहले base pairing कर सकते हैं। Ligase इस pairing को permanent bond में बदलता है। / Complementary bases of sticky ends can first base pair. Ligase converts this pairing into a permanent bond.
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How does ligase make sticky-end joining permanent?
C. phosphodiester bond बनाकरBy forming phosphodiester bond
Sticky ends पहले base pairing करते हैं। Ligase phosphodiester bond बनाकर stable recombinant DNA बनाता है। / Sticky ends first base pair. Ligase forms a phosphodiester bond to make stable recombinant DNA.
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In which condition can ligase reaction be less successful?
D. incompatible DNA ends होंIncompatible DNA ends are present
Incompatible DNA ends ठीक से pair नहीं कर पाएंगे। इससे ligation efficiency घट सकती है। / Incompatible DNA ends will not pair properly. This can reduce ligation efficiency.
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What problem may occur if there is no energy source in a ligation mixture?
A. bond formation प्रभावित हो सकती हैBond formation may be affected
Ligase को phosphodiester bond बनाने के लिए ऊर्जा चाहिए। ऊर्जा स्रोत न हो तो ligation कमजोर हो सकती है। / Ligase needs energy to form a phosphodiester bond. Without an energy source ligation can be weak.
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