Self-ligation cloning में क्यों unwanted है?
Why is self-ligation unwanted in cloning?
Explanation opens after your attempt
A. यह vector को insert के बिना बंद कर सकता हैIt can close vector without insert
Concept
In self-ligation the vector joins its own ends. This does not make the desired recombinant plasmid with insert.
Why this answer is correct
The correct answer is A. यह vector को insert के बिना बंद कर सकता है / It can close vector without insert. In self-ligation the vector joins its own ends. This does not make the desired recombinant plasmid with insert.
Exam Tip
Self-ligation में vector अपने ही सिरों से जुड़ जाता है। इससे desired insert वाला recombinant plasmid नहीं बनता।
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