Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 101-110 of 290 questions.
A student says ligase cuts DNA. What is the correct correction?
A. ligase DNA को जोड़ता हैLigase joins DNA
Ligase DNA cutting enzyme नहीं है। यह DNA fragments को जोड़ने वाला enzyme है। / Ligase is not a DNA-cutting enzyme. It is an enzyme that joins DNA fragments.
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Which enzyme is needed to join a cut plasmid and gene fragment?
A. DNA ligase
Cut plasmid और gene fragment को join करने के लिए DNA ligase उपयोग होता है। यह recombinant plasmid बनाने में मदद करता है। / DNA ligase is used to join a cut plasmid and gene fragment. It helps make a recombinant plasmid.
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Which reaction is catalyzed by ligase enzyme?
A. DNA ends के बीच joining reactionJoining reaction between DNA ends
DNA ligase DNA ends के बीच joining catalyze करता है। यह reaction phosphodiester bond formation से जुड़ी है। / DNA ligase catalyzes joining between DNA ends. This reaction is linked with phosphodiester bond formation.
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Which is the most correct substrate for DNA ligase?
A. nicked DNA
DNA ligase nicked DNA पर काम करके nick seal कर सकता है। यह DNA backbone को continuous बनाता है। / DNA ligase can act on nicked DNA and seal the nick. It makes the DNA backbone continuous.
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A cloning tube has vector and insert but no ligase. What will be missing?
A. stable phosphodiester joining
Ligase न होने पर permanent phosphodiester bond नहीं बनेगा। इसलिए stable recombinant DNA नहीं बनेगा। / Without ligase the permanent phosphodiester bond will not form. Therefore stable recombinant DNA will not form.
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Which condition is favorable for sticky-end ligation?
A. complementary overhangs present होंComplementary overhangs are present
Complementary overhangs sticky ends को पहले pair करने देते हैं। Active ligase फिर permanent bond बनाता है। / Complementary overhangs allow sticky ends to pair first. Active ligase then makes a permanent bond.
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What is the main challenge in blunt-end ligation?
A. complementary overhang absent होता हैComplementary overhang is absent
Blunt ends में overhang नहीं होता इसलिए initial base pairing support कम होता है। इसलिए ligation less efficient हो सकती है। / Blunt ends lack overhangs so initial base pairing support is low. Therefore ligation can be less efficient.
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Which enzyme is commonly used to join both sticky and blunt DNA ends?
A. T4 DNA ligase
T4 DNA ligase sticky और blunt दोनों प्रकार के DNA ends जोड़ सकता है। इसलिए cloning lab में यह popular है। / T4 DNA ligase can join both sticky and blunt DNA ends. Therefore it is popular in cloning labs.
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T4 DNA ligase uses energy from which molecule?
A. ATP
T4 DNA ligase ATP का use करता है। Energy phosphodiester bond बनाने में काम आती है। / T4 DNA ligase uses ATP. Energy is used to form a phosphodiester bond.
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E coli DNA ligase is commonly associated with which cofactor?
A. NAD plus
E coli DNA ligase सामान्यतः NAD plus dependent माना जाता है। T4 ligase ATP dependent होता है। / E coli DNA ligase is generally considered NAD plus dependent. T4 ligase is ATP dependent.
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