Blunt-end ligation में main challenge क्या है?

What is the main challenge in blunt-end ligation?

Explanation opens after your attempt
Correct Answer

A. complementary overhang absent होता हैComplementary overhang is absent

Step 1

Concept

Blunt ends lack overhangs so initial base pairing support is low. Therefore ligation can be less efficient.

Step 2

Why this answer is correct

The correct answer is A. complementary overhang absent होता है / Complementary overhang is absent. Blunt ends lack overhangs so initial base pairing support is low. Therefore ligation can be less efficient.

Step 3

Exam Tip

Blunt ends में overhang नहीं होता इसलिए initial base pairing support कम होता है। इसलिए ligation less efficient हो सकती है।

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Biotechnology Answer, Explanation and Revision Hints

Blunt-end ligation में main challenge क्या है? / What is the main challenge in blunt-end ligation?

Correct Answer: A. complementary overhang absent होता है / Complementary overhang is absent. Explanation: Blunt ends में overhang नहीं होता इसलिए initial base pairing support कम होता है। इसलिए ligation less efficient हो सकती है। / Blunt ends lack overhangs so initial base pairing support is low. Therefore ligation can be less efficient.

Which concept should I revise for this Biotechnology MCQ?

Blunt ends lack overhangs so initial base pairing support is low. Therefore ligation can be less efficient.

What exam hint can help solve this Biotechnology question?

Blunt ends में overhang नहीं होता इसलिए initial base pairing support कम होता है। इसलिए ligation less efficient हो सकती है।