Blunt-end ligation में main challenge क्या है?
What is the main challenge in blunt-end ligation?
Explanation opens after your attempt
A. complementary overhang absent होता हैComplementary overhang is absent
Concept
Blunt ends lack overhangs so initial base pairing support is low. Therefore ligation can be less efficient.
Why this answer is correct
The correct answer is A. complementary overhang absent होता है / Complementary overhang is absent. Blunt ends lack overhangs so initial base pairing support is low. Therefore ligation can be less efficient.
Exam Tip
Blunt ends में overhang नहीं होता इसलिए initial base pairing support कम होता है। इसलिए ligation less efficient हो सकती है।
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