Concept-wise Practice

blunt end ligation MCQ Questions for Class 11

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Practice Questions

4 questions tagged with blunt end ligation.

Blunt-end ligation में main challenge क्या है?

What is the main challenge in blunt-end ligation?

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Correct Answer

A. complementary overhang absent होता हैComplementary overhang is absent

Step 1

Concept

Blunt ends lack overhangs so initial base pairing support is low. Therefore ligation can be less efficient.

Step 2

Why this answer is correct

The correct answer is A. complementary overhang absent होता है / Complementary overhang is absent. Blunt ends lack overhangs so initial base pairing support is low. Therefore ligation can be less efficient.

Step 3

Exam Tip

Blunt ends में overhang नहीं होता इसलिए initial base pairing support कम होता है। इसलिए ligation less efficient हो सकती है।

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Blunt-end ligation को बेहतर करने के लिए किसकी जरूरत हो सकती है?

What may be needed to improve blunt-end ligation?

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Correct Answer

A. अधिक उपयुक्त ligase conditionsMore suitable ligase conditions

Step 1

Concept

Blunt ends lack overhangs so joining can be difficult. Suitable conditions can help ligation.

Step 2

Why this answer is correct

The correct answer is A. अधिक उपयुक्त ligase conditions / More suitable ligase conditions. Blunt ends lack overhangs so joining can be difficult. Suitable conditions can help ligation.

Step 3

Exam Tip

Blunt ends में overhang नहीं होता इसलिए joining कठिन हो सकती है। Suitable conditions ligation में मदद कर सकती हैं।

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मंद सिरों की लाइगेशन में कौन सी कठिनाई हो सकती है?

What difficulty can occur in blunt-end ligation?

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Correct Answer

A. पूरक उभार नहीं होताThere is no complementary overhang

Step 1

Concept

Blunt ends do not have single-stranded overhangs. Therefore they do not hold easily by base pairing first.

Step 2

Why this answer is correct

The correct answer is A. पूरक उभार नहीं होता / There is no complementary overhang. Blunt ends do not have single-stranded overhangs. Therefore they do not hold easily by base pairing first.

Step 3

Exam Tip

मंद सिरों में एकल तंतु उभार नहीं होता। इसलिए वे पहले से आधार युग्मन द्वारा आसानी से नहीं टिकते।

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किस scenario में blunt-end ligation sticky-end ligation से कठिन हो सकती है?

In which scenario can blunt-end ligation be harder than sticky-end ligation?

Explanation opens after your attempt
Correct Answer

A. जब complementary overhang मौजूद न होंWhen complementary overhangs are absent

Step 1

Concept

Blunt ends lack overhangs so base pairing support is absent. This can make ligation comparatively harder.

Step 2

Why this answer is correct

The correct answer is A. जब complementary overhang मौजूद न हों / When complementary overhangs are absent. Blunt ends lack overhangs so base pairing support is absent. This can make ligation comparatively harder.

Step 3

Exam Tip

Blunt ends में overhang नहीं होते इसलिए base pairing सहायता नहीं मिलती। इससे ligation तुलनात्मक रूप से कठिन हो सकती है।

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