Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
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Showing 111-120 of 290 questions.
Which option gives the correct source of T4 DNA ligase?
A. T4 bacteriophage
T4 DNA ligase T4 bacteriophage से जुड़ा है। इसका प्रयोग recombinant DNA cloning में किया जाता है। / T4 DNA ligase is linked with T4 bacteriophage. It is used in recombinant DNA cloning.
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What does DNA ligase do in replication?
A. Okazaki fragments को join करता हैJoins Okazaki fragments
Replication में DNA ligase Okazaki fragments को join करता है। यह lagging strand को continuous बनाता है। / In replication DNA ligase joins Okazaki fragments. It makes the lagging strand continuous.
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Which enzyme makes fragments of the lagging strand continuous?
A. DNA ligase
Lagging strand पर बने Okazaki fragments को DNA ligase जोड़ता है। इससे DNA strand continuous बनता है। / DNA ligase joins Okazaki fragments formed on the lagging strand. This makes the DNA strand continuous.
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DNA ligase directly helps in repair of which type of DNA damage?
A. single-strand nick
DNA ligase single-strand nick को seal कर सकता है। यह DNA backbone continuity restore करता है। / DNA ligase can seal a single-strand nick. It restores DNA backbone continuity.
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Which statement correctly describes roles of ligase and polymerase?
A. polymerase strand बढ़ाता है और ligase nick seal करता हैPolymerase extends strand and ligase seals nick
Polymerase nucleotides जोड़कर strand बढ़ाता है। Ligase fragments के बीच backbone nick seal करता है। / Polymerase extends a strand by adding nucleotides. Ligase seals backbone nicks between fragments.
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Which is the correct matching between ligase and helicase?
A. ligase joins DNA और helicase unwinds DNALigase joins DNA and helicase unwinds DNA
Helicase DNA double helix को खोलता है। Ligase DNA fragments को जोड़ता है। / Helicase unwinds the DNA double helix. Ligase joins DNA fragments.
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Which option gives an incorrect role of ligase?
D. Cutting DNA at restriction site
Restriction site पर DNA cutting restriction enzyme करता है। Ligase DNA ends joining करता है। / DNA cutting at restriction sites is done by restriction enzymes. Ligase joins DNA ends.
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Which option gives the correct role of ligase in recombinant DNA?
A. foreign DNA और vector को covalently join करनाCovalently joining foreign DNA and vector
Ligase foreign DNA और vector backbone के बीच covalent phosphodiester bond बनाता है। इससे recombinant DNA stable होता है। / Ligase forms covalent phosphodiester bonds between foreign DNA and vector backbone. This makes recombinant DNA stable.
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What is the purpose of buffer in a ligation mixture?
A. enzyme के लिए suitable pH और salts देनाProviding suitable pH and salts for the enzyme
Buffer ligase activity के लिए सही environment देता है। Wrong buffer ligation efficiency घटा सकता है। / Buffer gives the right environment for ligase activity. Wrong buffer can reduce ligation efficiency.
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What can be the role of Mg ion in DNA ligase reaction?
A. enzyme activity में सहायक cofactorA helper cofactor for enzyme activity
कई ligation reactions में magnesium ion enzyme activity के लिए महत्वपूर्ण होता है। Practical buffer में ऐसे ions शामिल हो सकते हैं। / In many ligation reactions magnesium ion is important for enzyme activity. Practical buffers may include such ions.
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