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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 71/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 46

DNA methylation के कारण restriction digest fail क्यों हो सकता है?

Why can restriction digest fail due to DNA methylation?

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A. methylation enzyme recognition रोक सकती हैMethylation can block enzyme recognition

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कुछ enzymes methylated recognition sites नहीं काटते। Methylation sensitivity enzyme choice में देखनी चाहिए। / Some enzymes do not cut methylated recognition sites. Methylation sensitivity should be checked in enzyme choice.

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Question 72/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 46

Methylation-sensitive enzyme क्या करता है?

What does a methylation-sensitive enzyme do?

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A. methylated site पर cutting बदल सकती हैIts cutting can change at methylated site

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Methylation-sensitive enzymes methylated DNA को कम या नहीं काट सकते। यह digestion pattern बदल सकता है। / Methylation-sensitive enzymes may cut methylated DNA poorly or not at all. This can change digestion pattern.

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Question 73/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 46

Restriction digestion में substrate DNA का अर्थ क्या है?

What does substrate DNA mean in restriction digestion?

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A. वह DNA जिसे enzyme काटेगाThe DNA that enzyme will cut

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Substrate DNA reaction में cutting target होता है। Enzyme recognition site इसी DNA पर खोजता है। / Substrate DNA is the cutting target in the reaction. The enzyme looks for recognition site on this DNA.

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Question 74/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 46

Linear DNA और circular DNA digestion patterns अलग क्यों हो सकते हैं?

Why can linear and circular DNA digestion patterns differ?

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A. उनकी shape और cut site arrangement अलग होती हैTheir shape and cut site arrangement differ

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Circular DNA एक cut से linear हो सकता है जबकि linear DNA में fragment count अलग हो सकता है। Shape pattern को प्रभावित करती है। / Circular DNA can become linear with one cut while linear DNA can show different fragment counts. Shape affects pattern.

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Question 75/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 46

Supercoiled plasmid gel पर linear plasmid से अलग क्यों दिख सकता है?

Why can supercoiled plasmid look different from linear plasmid on gel?

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A. supercoiled shape migration बदलती हैSupercoiled shape changes migration

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Plasmid conformation gel mobility को प्रभावित करती है। इसलिए uncut plasmid के bands अलग दिख सकते हैं। / Plasmid conformation affects gel mobility. Therefore uncut plasmid bands can look different.

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Question 76/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 46

Restriction digest के बाद vector linearization का अर्थ क्या है?

What does vector linearization after restriction digest mean?

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A. circular vector को काटकर linear बनानाCutting circular vector to make it linear

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Linearized vector insert ligation के लिए तैयार हो सकता है। सही site पर cut होना जरूरी है। / A linearized vector can be ready for insert ligation. Cutting at the correct site is necessary.

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Question 77/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 46

Vector self-ligation रोकने में कौन सा treatment मदद कर सकता है?

Which treatment can help prevent vector self-ligation?

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B. alkaline phosphatase treatment

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Alkaline phosphatase vector ends से phosphate हटाकर self-ligation घटा सकता है। यह cloning efficiency में मदद करता है। / Alkaline phosphatase removes phosphate from vector ends and can reduce self-ligation. This helps cloning efficiency.

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Question 78/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 46

Restriction digestion के बाद insert purification क्यों की जाती है?

Why is insert purification done after restriction digestion?

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A. insert को बाकी DNA और enzyme से अलग करने के लिएTo separate insert from other DNA and enzyme

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Purification desired insert को साफ करती है। इससे ligation और cloning result बेहतर हो सकते हैं। / Purification cleans the desired insert. This can improve ligation and cloning results.

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Question 79/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 46

Gel extraction में UV light exposure बहुत ज्यादा होने से क्या नुकसान हो सकता है?

What harm can too much UV light exposure cause during gel extraction?

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A. DNA damage हो सकता हैDNA can be damaged

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UV exposure DNA को damage कर सकता है। Desired band को जल्दी और सावधानी से excise करें। / UV exposure can damage DNA. Excise the desired band quickly and carefully.

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Question 80/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 46

Restriction enzyme reaction के बाद cleanup कब helpful है?

When is cleanup after restriction enzyme reaction helpful?

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A. ligation या PCR से पहले inhibitors हटाने के लिएTo remove inhibitors before ligation or PCR

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Cleanup enzyme buffer salts और छोटे fragments हटाने में मदद कर सकता है। इससे next step efficient हो सकता है। / Cleanup can remove enzyme buffer salts and small fragments. This can make the next step efficient.

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