Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 81-90 of 592 questions.
What can be an example of a negative control in restriction digestion?
B. DNA without enzyme
DNA without enzyme uncut pattern दिखाता है। इससे cutting effect compare किया जाता है। / DNA without enzyme shows the uncut pattern. It is used to compare the cutting effect.
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Why can a positive control digest be set up?
A. enzyme काम कर रहा है या नहीं देखने के लिएTo check whether enzyme is working
Known DNA substrate पर expected cut enzyme activity verify करता है। यह troubleshooting में useful है। / Expected cutting on a known DNA substrate verifies enzyme activity. This is useful in troubleshooting.
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If even the positive control does not cut what should be suspected?
A. enzyme buffer या condition problemEnzyme buffer or condition problem
Positive control failure enzyme या reaction condition में problem दिखाता है। Buffer temperature और enzyme storage check करें। / Positive control failure indicates a problem with enzyme or reaction condition. Check buffer temperature and enzyme storage.
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If test DNA does not cut but positive control cuts what can be the reason?
A. test DNA में site absent या methylated हो सकती हैTest DNA may lack site or be methylated
Positive control enzyme activity दिखाता है। Test DNA में recognition site या methylation issue हो सकता है। / Positive control shows enzyme activity. Test DNA may have recognition site or methylation issue.
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What is useful to check when restriction enzyme lot or batch changes?
A. activity और expected digestionActivity and expected digestion
New batch की performance verify करना reliable results के लिए अच्छा practice है। Control digest उपयोगी है। / Verifying performance of a new batch is good practice for reliable results. Control digest is useful.
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What effect can incorrect pH have in a restriction enzyme reaction?
A. enzyme activity घट सकती हैEnzyme activity can decrease
Enzymes specific pH range में best work करते हैं। Buffer correct pH maintain करता है। / Enzymes work best in a specific pH range. Buffer maintains the correct pH.
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Why is salt concentration important in a restriction enzyme reaction?
A. enzyme specificity और activity को प्रभावित कर सकता हैIt can affect enzyme specificity and activity
Salt condition enzyme binding और cutting को प्रभावित करती है। Recommended buffer use करें। / Salt condition affects enzyme binding and cutting. Use the recommended buffer.
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Why is restriction enzyme kept on ice?
A. enzyme activity और stability बचाने के लिएTo preserve enzyme activity and stability
Restriction enzymes heat sensitive हो सकते हैं। Ice पर रखने से activity loss कम होता है। / Restriction enzymes can be heat sensitive. Keeping on ice reduces activity loss.
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Why is the reaction tube mixed gently?
A. DNA और enzyme evenly mix करने के लिए without shearingTo mix DNA and enzyme evenly without shearing
Gentle mixing reaction components मिलाता है और DNA damage से बचाता है। Harsh vortexing avoid करें। / Gentle mixing combines reaction components and avoids DNA damage. Avoid harsh vortexing.
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Why should vector ends be compatible before ligation after restriction digestion?
A. DNA ligase तभी efficient joining कर पाएगाDNA ligase can then join efficiently
Compatible ends base pairing और ligation को आसान बनाते हैं। Incompatible ends cloning failure दे सकते हैं। / Compatible ends make base pairing and ligation easier. Incompatible ends can cause cloning failure.
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