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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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Cutting of DNA Practice Questions

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Question 101/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 47

Restriction site insert के अंदर भी हो तो क्या समस्या हो सकती है?

What problem can occur if a restriction site is also inside the insert?

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Correct Answer

D. insert unwanted fragments में कट सकता हैInsert can cut into unwanted fragments

Explanation

Simple Explanation

अगर chosen enzyme insert के अंदर cut करे तो desired gene टूट सकता है। Enzyme choice से पहले sequence check करें। / If the chosen enzyme cuts inside the insert the desired gene can break. Check sequence before enzyme choice.

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Question 102/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 47

Enzyme choice से पहले insert sequence क्यों check की जाती है?

Why is insert sequence checked before enzyme choice?

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Correct Answer

A. internal restriction sites से बचने के लिएTo avoid internal restriction sites

Explanation

Simple Explanation

Insert में chosen enzyme site हो तो insert टूट सकता है। इसलिए sequence analysis cloning planning में जरूरी है। / If the chosen enzyme site is inside the insert the insert can break. Therefore sequence analysis is needed in cloning planning.

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Question 103/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 47

Restriction digestion से orientation check कैसे हो सकती है?

How can restriction digestion help check orientation?

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Correct Answer

B. different band sizes pattern सेBy different band size pattern

Explanation

Simple Explanation

Insert orientation के अनुसार restriction sites की distance बदल सकती है। Digest pattern से orientation का संकेत मिलता है। / Depending on insert orientation distances between restriction sites can change. Digest pattern can indicate orientation.

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Question 104/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 47

Diagnostic digest में single enzyme use कब useful हो सकता है?

When can using a single enzyme be useful in diagnostic digest?

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Correct Answer

C. plasmid linearization या simple size check के लिएFor plasmid linearization or simple size check

Explanation

Simple Explanation

Single cut plasmid को linear form में बदल सकता है। इससे approximate plasmid size check हो सकता है। / A single cut can convert plasmid into linear form. This can help check approximate plasmid size.

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Question 105/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 47

Diagnostic digest में two-enzyme cut का लाभ क्या है?

What is the benefit of a two-enzyme cut in diagnostic digest?

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Correct Answer

D. insert size या orientation बेहतर check करनाBetter checking insert size or orientation

Explanation

Simple Explanation

Two enzymes expected fragment pattern देते हैं। इससे clone verification अधिक informative हो सकता है। / Two enzymes give an expected fragment pattern. This can make clone verification more informative.

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Question 106/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 47

Restriction digest में plasmid uncut band क्यों दिखाई दे सकता है?

Why can an uncut plasmid band appear in restriction digest?

Explanation opens after your attempt

Correct Answer

A. incomplete digestion के कारणDue to incomplete digestion

Explanation

Simple Explanation

Incomplete digestion में कुछ plasmid uncut रह सकता है। Gel पर uncut और cut forms साथ दिख सकते हैं। / In incomplete digestion some plasmid can remain uncut. Uncut and cut forms can appear together on gel.

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Question 107/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 47

Restriction digest में smear दिखना किसका संकेत हो सकता है?

What can smear in a restriction digest indicate?

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Correct Answer

B. degraded DNA या nonspecific cuttingDegraded DNA or nonspecific cutting

Explanation

Simple Explanation

Smear DNA degradation या star activity जैसी समस्या दिखा सकता है। Reaction conditions check करें। / A smear can show DNA degradation or a problem like star activity. Check reaction conditions.

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Question 108/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 47

Gel पर expected insert band नहीं दिखे तो क्या संभव है?

What is possible if expected insert band is not seen on gel?

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Correct Answer

A. insert absent या digestion failed हो सकता हैInsert may be absent or digestion failed

Explanation

Simple Explanation

Expected band न होना wrong clone partial digestion या low DNA amount का संकेत हो सकता है। Controls से confirm करें। / Absence of expected band can indicate wrong clone partial digestion or low DNA amount. Confirm with controls.

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Question 109/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 47

Restriction digestion के बाद band बहुत faint हो तो क्या कारण हो सकता है?

What can cause a very faint band after restriction digestion?

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Correct Answer

B. कम DNA amountLow DNA amount

Explanation

Simple Explanation

Low DNA loading से faint bands दिखते हैं। DNA concentration और loading amount check करें। / Low DNA loading gives faint bands. Check DNA concentration and loading amount.

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Question 110/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 47

Restriction digest के बाद बहुत bright smear क्या बताता है?

What can a very bright smear after restriction digest show?

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Correct Answer

C. overloaded DNA या degradationOverloaded DNA or degradation

Explanation

Simple Explanation

बहुत ज्यादा DNA loading या degraded DNA smear दे सकता है। Gel loading और DNA quality check करें। / Too much DNA loading or degraded DNA can give a smear. Check gel loading and DNA quality.

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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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Cutting of DNA Practice Questions

Restriction site insert के अंदर भी हो तो क्या समस्या हो सकती है?

Simple Explanation

Enzyme choice से पहले insert sequence क्यों check की जाती है?

Simple Explanation

Restriction digestion से orientation check कैसे हो सकती है?

Simple Explanation

Diagnostic digest में single enzyme use कब useful हो सकता है?

Simple Explanation

Diagnostic digest में two-enzyme cut का लाभ क्या है?

Simple Explanation

Restriction digest में plasmid uncut band क्यों दिखाई दे सकता है?

Simple Explanation

Restriction digest में smear दिखना किसका संकेत हो सकता है?

Simple Explanation

Gel पर expected insert band नहीं दिखे तो क्या संभव है?

Simple Explanation

Restriction digestion के बाद band बहुत faint हो तो क्या कारण हो सकता है?

Simple Explanation

Restriction digest के बाद बहुत bright smear क्या बताता है?

Simple Explanation

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Biotechnology Subject

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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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Cutting of DNA Practice Questions

Restriction site insert के अंदर भी हो तो क्या समस्या हो सकती है?

Simple Explanation

Enzyme choice से पहले insert sequence क्यों check की जाती है?

Simple Explanation

Restriction digestion से orientation check कैसे हो सकती है?

Simple Explanation

Diagnostic digest में single enzyme use कब useful हो सकता है?

Simple Explanation

Diagnostic digest में two-enzyme cut का लाभ क्या है?

Simple Explanation

Restriction digest में plasmid uncut band क्यों दिखाई दे सकता है?

Simple Explanation

Restriction digest में smear दिखना किसका संकेत हो सकता है?

Simple Explanation

Gel पर expected insert band नहीं दिखे तो क्या संभव है?

Simple Explanation

Restriction digestion के बाद band बहुत faint हो तो क्या कारण हो सकता है?

Simple Explanation

Restriction digest के बाद बहुत bright smear क्या बताता है?

Simple Explanation

Cutting of DNA Learning Links

Processes Of Recombinant Dna Technology Chapter

Easy Cutting of DNA Test

Biotechnology Subject

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