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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 511/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion में enzyme carryover ligation में क्यों risk है?

Why is enzyme carryover risky in ligation after restriction digestion?

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A. ligated DNA को फिर cut कर सकता हैIt can cut ligated DNA again

Explanation

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Active restriction enzyme newly ligated DNA को काट सकता है। इसलिए inactivation या purification use करें। / Active restriction enzyme can cut newly ligated DNA. Therefore use inactivation or purification.

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Question 512/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion के बाद agarose gel से fragment निकालते समय low-melt agarose कब useful हो सकता है?

When can low-melt agarose be useful while extracting fragment after restriction digestion?

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A. sensitive DNA recovery मेंIn sensitive DNA recovery

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Low-melt agarose DNA recovery को आसान और gentle बना सकता है। यह cloning fragments के लिए useful हो सकता है। / Low-melt agarose can make DNA recovery easier and gentler. It can be useful for cloning fragments.

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Question 513/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction fragment gel extraction में gel slice को छोटा रखना क्यों अच्छा है?

Why is it good to keep gel slice small in restriction fragment gel extraction?

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A. binding capacity और impurity load बेहतर रहता हैBinding capacity and impurity load remain better

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छोटा gel slice purification को efficient बनाता है। जरूरत से ज्यादा agarose recovery घटा सकता है। / A small gel slice makes purification efficient. Excess agarose can reduce recovery.

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Question 514/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Gel extraction में band cut करते समय scalpel clean क्यों होना चाहिए?

Why should the scalpel be clean while cutting band in gel extraction?

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A. DNA contamination रोकने के लिएTo prevent DNA contamination

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Dirty scalpel unwanted DNA या nuclease ला सकता है। Clean tool fragment purity बचाता है। / A dirty scalpel can introduce unwanted DNA or nuclease. A clean tool protects fragment purity.

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Question 515/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction fragment recovery में UV exposure को कम क्यों रखा जाता है?

Why is UV exposure minimized during restriction fragment recovery?

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A. DNA damage कम करने के लिएTo reduce DNA damage

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UV DNA में damage कर सकता है और cloning efficiency घटा सकता है। Band जल्दी excise करें। / UV can damage DNA and reduce cloning efficiency. Excise the band quickly.

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Question 516/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Blue-light compatible stain का cloning fragment recovery में लाभ क्या है?

What is the benefit of blue-light compatible stain in cloning fragment recovery?

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A. UV damage risk घट सकता हैUV damage risk can decrease

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Blue light UV की तुलना में DNA damage कम कर सकता है। यह downstream cloning के लिए helpful है। / Blue light can reduce DNA damage compared with UV. It is helpful for downstream cloning.

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Question 517/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digest के बाद desired fragment gel से निकालने से पहले band identity कैसे confirm करें?

How should band identity be confirmed before extracting desired fragment from gel after restriction digest?

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A. ladder और expected map से compare करकेBy comparing with ladder and expected map

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Band size expected fragment से match होना चाहिए। गलत band purification cloning को fail कर सकती है। / Band size should match expected fragment. Purifying a wrong band can fail cloning.

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Question 518/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction fragment purification के बाद elution volume कम रखने से क्या फायदा हो सकता है?

What can be the benefit of low elution volume after restriction fragment purification?

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A. DNA concentration बढ़ सकती हैDNA concentration can increase

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कम volume में recovered DNA अधिक concentrated होता है। लेकिन बहुत कम volume recovery घटा सकता है। / Recovered DNA is more concentrated in low volume. But too low volume can reduce recovery.

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Question 519/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Purified restriction fragment को ligation से पहले quantify क्यों करते हैं?

Why is purified restriction fragment quantified before ligation?

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A. insert-vector ratio सही रखने के लिएTo keep insert-vector ratio correct

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Ligation setup में fragment amount और molar ratio important हैं। Quantification cloning efficiency सुधारती है। / Fragment amount and molar ratio are important in ligation setup. Quantification improves cloning efficiency.

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Question 520/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion product में small primer-like fragments क्यों problematic हो सकते हैं?

Why can small primer-like fragments be problematic in restriction digestion product?

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A. वे ligation में unwanted competition कर सकते हैंThey can cause unwanted competition in ligation

Explanation

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छोटे DNA pieces compatible ends लेकर ligation reaction disturb कर सकते हैं। Cleanup इन्हें हटाने में मदद करता है। / Small DNA pieces with compatible ends can disturb ligation. Cleanup helps remove them.

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