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Biotechnology Cutting of DNA MCQ Questions for Class 11 General

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01 Practice Topic Isolation of DNA 294 MCQs

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Question 521/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion product में salts बचने से ligation पर क्या असर हो सकता है?

What effect can remaining salts in restriction digestion product have on ligation?

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A. ligation efficiency घट सकती हैLigation efficiency can decrease

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Excess salts ligase और DNA end interaction को affect कर सकते हैं। Purification और wash step जरूरी हैं। / Excess salts can affect ligase and DNA end interaction. Purification and wash step are necessary.

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Question 522/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion product में residual ethanol ligation को कैसे प्रभावित कर सकता है?

How can residual ethanol affect ligation in restriction digestion product?

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A. ligase activity inhibit कर सकता हैIt can inhibit ligase activity

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Residual ethanol downstream enzyme reactions को रोक सकता है। DNA pellet या column membrane को dry करें। / Residual ethanol can inhibit downstream enzyme reactions. Dry the DNA pellet or column membrane.

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Question 523/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion के बाद DNA ends को damage होने से कैसे बचाया जा सकता है?

How can DNA ends be protected from damage after restriction digestion?

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A. gentle handling और nuclease-free condition सेBy gentle handling and nuclease-free condition

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DNA ends ligation के लिए important हैं। Rough handling contamination और UV damage से बचें। / DNA ends are important for ligation. Avoid rough handling contamination and UV damage.

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Question 524/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction cut insert को long-term store करना हो तो क्या ध्यान रखें?

What should be considered if restriction-cut insert must be stored long-term?

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A. low temperature और nuclease-free bufferLow temperature and nuclease-free buffer

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Low temperature DNA stability बढ़ाता है। Label और aliquot भी useful हैं। / Low temperature increases DNA stability. Label and aliquot are also useful.

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Question 525/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion product को बार-बार freeze-thaw करने से क्या risk है?

What is the risk of repeated freeze-thaw of restriction digestion product?

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A. DNA quality या ends प्रभावित हो सकते हैंDNA quality or ends can be affected

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Repeated freeze-thaw DNA integrity को कम कर सकता है। Aliquots बनाकर store करें। / Repeated freeze-thaw can reduce DNA integrity. Store by making aliquots.

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Question 526/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion product labeling में क्या जरूर लिखना चाहिए?

What should be written in restriction digestion product labeling?

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A. enzyme name fragment identity और dateEnzyme name fragment identity and date

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Proper label future ligation और troubleshooting में मदद करता है। बिना label sample mix-up हो सकता है। / Proper label helps future ligation and troubleshooting. Without label sample mix-up can occur.

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Question 527/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion product का storage label क्यों जरूरी है?

Why is storage label of restriction digestion product necessary?

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A. sample identity और enzyme history track करने के लिएTo track sample identity and enzyme history

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Label से पता चलता है fragment किस enzyme से बना है। यह later cloning decision में useful है। / The label shows which enzyme produced the fragment. This is useful in later cloning decisions.

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Question 528/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion में chain-of-sample record कब useful है?

When is chain-of-sample record useful in restriction digestion?

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A. multiple samples और clones handle करते समयWhile handling multiple samples and clones

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कई samples में mix-up risk बढ़ता है। Tracking record correct identity बनाए रखता है। / Mix-up risk increases with many samples. Tracking record maintains correct identity.

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Question 529/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion experiment में negative result को छिपाना क्यों गलत है?

Why is hiding a negative result in restriction digestion experiment wrong?

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A. यह scientific honesty के खिलाफ हैIt is against scientific honesty

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Negative result troubleshooting के लिए useful होता है। सही reporting responsible science का भाग है। / A negative result is useful for troubleshooting. Correct reporting is part of responsible science.

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Question 530/592 Easy Biotechnology Processes of Recombinant DNA Technology Cutting of DNA Class 11 Level 55

Restriction digestion में failed reaction को repeat करते समय क्या बदलना चाहिए?

What should be changed while repeating a failed restriction digestion?

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A. suspected variable जैसे buffer enzyme amount या DNA puritySuspected variable such as buffer enzyme amount or DNA purity

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Troubleshooting में एक या सीमित variables बदलना बेहतर है। इससे cause समझ आता है। / It is better to change one or limited variables in troubleshooting. This helps identify the cause.

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